LOCUS       sequence-340322-        9101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vitro transcription of Cas9n-RT-T2A-PuroR synthetic construct for
            PRIME editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9101)
  TITLE     Verstreken lab plasmids
REFERENCE   2  (bases 1 to 9101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIVTR(T7A)-Cas9n-RT-T2A-PuroR
FEATURES             Location/Qualifiers
     source          1..9101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             141..161
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(162..2675,2676..2678,2679..4262)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             162..4262
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6435..6455
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6465..6518
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6528..7127
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6528..6547)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7024..7044
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     rep_origin      complement(7346..7934)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7426..7445)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8105..8896,8897..8965))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8728..8747
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8966..9070)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taatacgact cactataagg acatttgctt ctgacacaac tgtgttcact agcaacctca
       61 aacagaattc gagctcggta cccggggatc ctctagagtc caccatgaaa cggacagccg
      121 acggaagcga gttcgagtca ccaaagaaga agcggaaagt cgacaagaag tacagcatcg
      181 gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc
      241 ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga
      301 tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg
      361 ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca
      421 acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg
      481 aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct
      541 accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca
      601 aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact
      661 tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc
      721 tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg
      781 ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc
      841 agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg agcctgggcc
      901 tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca
      961 aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg
     1021 acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag
     1081 tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc
     1141 accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca
     1201 aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca
     1261 gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg
     1321 aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg
     1381 gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag
     1441 atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca
     1501 tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa
     1561 agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg
     1621 cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc
     1681 tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga
     1741 aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca
     1801 tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact
     1861 acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca
     1921 acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg
     1981 acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg
     2041 acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga
     2101 tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca
     2161 acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct
     2221 tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca
     2281 tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg
     2341 ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg
     2401 tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc
     2461 agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca
     2521 tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga
     2581 acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac
     2641 tggacatcaa ccggctgtcc gactacgatg tggacgctat cgtgcctcag agctttctga
     2701 aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg
     2761 acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga
     2821 acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc
     2881 tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca
     2941 caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca
     3001 agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga
     3061 aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct
     3121 acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt
     3181 tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg
     3241 aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga
     3301 ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg
     3361 aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga
     3421 gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag
     3481 agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc
     3541 ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca
     3601 aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca
     3661 tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca
     3721 aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa
     3781 acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc
     3841 tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct
     3901 cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg
     3961 agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg
     4021 acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga
     4081 atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg
     4141 acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga
     4201 tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggtg
     4261 actctggagg atctagcgga ggatcctctg gcagcgagac accaggaaca agcgagtcag
     4321 caacaccaga gagcagtggc ggcagcagcg gcggcagcag caccctaaat atagaagatg
     4381 agtatcggct acatgagacc tcaaaagagc cagatgtttc tctagggtcc acatggctgt
     4441 ctgattttcc tcaggcctgg gcggaaaccg ggggcatggg actggcagtt cgccaagctc
     4501 ctctgatcat acctctgaaa gcaacctcta cccccgtgtc cataaaacaa taccccatgt
     4561 cacaagaagc cagactgggg atcaagcccc acatacagag actgttggac cagggaatac
     4621 tggtaccctg ccagtccccc tggaacacgc ccctgctacc cgttaagaaa ccagggacta
     4681 atgattatag gcctgtccag gatctgagag aagtcaacaa gcgggtggaa gacatccacc
     4741 ccaccgtgcc caacccttac aacctcttga gcgggctccc accgtcccac cagtggtaca
     4801 ctgtgcttga tttaaaggat gcctttttct gcctgagact ccaccccacc agtcagcctc
     4861 tcttcgcctt tgagtggaga gatccagaga tgggaatctc aggacaattg acctggacca
     4921 gactcccaca gggtttcaaa aacagtccca ccctgtttaa tgaggcactg cacagagacc
     4981 tagcagactt ccggatccag cacccagact tgatcctgct acagtacgtg gatgacttac
     5041 tgctggccgc cacttctgag ctagactgcc aacaaggtac tcgggccctg ttacaaaccc
     5101 tagggaacct cgggtatcgg gcctcggcca agaaagccca aatttgccag aaacaggtca
     5161 agtatctggg gtatcttcta aaagagggtc agagatggct gactgaggcc agaaaagaga
     5221 ctgtgatggg gcagcctact ccgaagaccc ctcgacaact aagggagttc ctagggaagg
     5281 caggcttctg tcgcctcttc atccctgggt ttgcagaaat ggcagccccc ctgtaccctc
     5341 tcaccaaacc ggggactctg tttaattggg gcccagacca acaaaaggcc tatcaagaaa
     5401 tcaagcaagc tcttctaact gccccagccc tggggttgcc agatttgact aagccctttg
     5461 aactctttgt cgacgagaag cagggctacg ccaaaggtgt cctaacgcaa aaactgggac
     5521 cttggcgtcg gccggtggcc tacctgtcca aaaagctaga cccagtagca gctgggtggc
     5581 ccccttgcct acggatggta gcagccattg ccgtactgac aaaggatgca ggcaagctaa
     5641 ccatgggaca gccactagtc attctggccc cccatgcagt agaggcacta gtcaaacaac
     5701 cccccgaccg ctggctttcc aacgcccgga tgactcacta tcaggccttg cttttggaca
     5761 cggaccgggt ccagttcgga ccggtggtag ccctgaaccc ggctacgctg ctcccactgc
     5821 ctgaggaagg gctgcaacac aactgccttg atatcctggc cgaagcccac ggaacccgac
     5881 ccgacctaac ggaccagccg ctcccagacg ccgaccacac ctggtacacg gatggaagca
     5941 gtctcttaca agagggacag cgtaaggcgg gagctgcggt gaccaccgag accgaggtaa
     6001 tctgggctaa agccctgcca gccgggacat ccgctcagcg ggctgaactg atagcactca
     6061 cccaggccct aaagatggca gaaggtaaga agctaaatgt ttatactgat agccgttatg
     6121 cttttgctac tgcccatatc catggagaaa tatacagaag gcgtgggtgg ctcacatcag
     6181 aaggcaaaga gatcaaaaat aaagacgaga tcttggccct actaaaagcc ctctttctgc
     6241 ccaaaagact tagcataatc cattgtccag gacatcaaaa gggacacagc gccgaggcta
     6301 gaggcaaccg gatggctgac caagcggccc gaaaggcagc catcacagag actccagaca
     6361 cctctaccct cctcatagaa aattcatcac cctctggcgg ctcaaaaaga accgccgacg
     6421 gcagcgaatt cgagcccaag aagaagagga aagtcggcag cggagagggc agaggaagtc
     6481 ttctaacatg cggtgacgtg gaggagaatc ccggccctag gctcgagatg accgagtaca
     6541 agcccacggt gcgcctcgcc actcgcgacg acgtccccag ggccgtacgc accctcgccg
     6601 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     6661 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     6721 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     6781 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     6841 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     6901 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     6961 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     7021 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     7081 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagac ctgcaggcat
     7141 gcaagcttgc tcgctttctt gctgtccaat ttctattaaa ggttcctttg ttccctaagt
     7201 ccaactacta aactggggga tattatgaag ggccttgagc atctggattc tgcctaataa
     7261 aaaacattta ttttcattgc aaaaccatgg gagcaaaagg ccagcaaaag gccaggaacc
     7321 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7381 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7441 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7501 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7561 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7621 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7681 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7741 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     7801 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7861 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7921 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7981 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8041 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8101 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8161 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8221 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8281 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8341 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8401 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8461 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8521 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8581 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8641 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8701 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8761 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8821 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8881 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8941 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9001 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9061 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
