LOCUS       sequence-340330-        9473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  encodes NL63 spike protein, for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Dacon C, Tucker C, Peng L, Lee CD, Lin TH, Yuan M, Cong Y, Wang L, 
            Purser L, Williams JK, Pyo CW, Kosik I, Hu Z, Zhao M, Mohan D, 
            Cooper AJR, Peterson M, Skinner J, Dixit S, Kollins E, Huzella L, 
            Perry D, Byrum R, Lembirik S, Drawbaugh D, Eaton B, Zhang Y, Yang 
            ES, Chen M, Leung K, Weinberg RS, Pegu A, Geraghty DE, Davidson E, 
            Douagi I, Moir S, Yewdell JW, Schmaljohn C, Crompton PD, Holbrook 
            MR, Nemazee D, Mascola JR, Wilson IA, Tan J
  TITLE     Broadly neutralizing antibodies target the coronavirus fusion 
            peptide.
  JOURNAL   Science. 2022 Jul 12:eabq3773. doi: 10.1126/science.abq3773.
  PUBMED    35857439
REFERENCE   2  (bases 1 to 9473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2022 Jul 12:eabq3773. doi: 10.1126/science.abq3773."
COMMENT     Sequence Label: pcDNA3.3-NL63-D14
FEATURES             Location/Qualifiers
     source          1..9473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(4810..4829)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4854..4902
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5104..5532
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5191..5210)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5401..5422
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5541..5561)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5546..5875
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5726..5861
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5788..5807
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5942..6736
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5996..6015)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6606..6625
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6912..7033
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     7003..7022
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7082..7098)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7082..7098)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7095..7117)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7106..7122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7130..7136,7137..7154,7155..7160))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7175..7196
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7313..7330)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7484..8072)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7564..7583)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8243..9034,9035..9103))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8866..8885
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9104..9208)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9283..9302)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg gactgagctc cttaaggtta actctagaga attcggatcc gccaccatga
      721 agctgttcct gatcctgctg gtgctgcctc tggccagctg cttcttcacc tgtaacagca
      781 acgccaacct gtccatgctc cagctgggcg tgccagacaa tagcagcacc atcgtgacag
      841 gcctgctgcc tacacactgg ttctgcgcca atcagagcac cagcgtgtac agcgccaacg
      901 gcttcttcta catcgacgtg ggcaaccaca gaagcgcctt cgcactgcac accggctact
      961 acgatgccaa ccagtactac atctacgtga ccaacgagat cggcctgaac gccagcgtga
     1021 ccctgaagat ctgcaagttc agccggaata ccaccttcga ctttctgagc aacgcctcca
     1081 gcagcttcga ctgcatcgtg aacctgctgt tcaccgagca gctgggagcc cctctgggca
     1141 tcacaatttc tggcgaaaca gtgcggctgc acctgtacaa cgtgacccgg accttttatg
     1201 tgccagccgc ctacaagctg accaagctga gcgtgaagtg ctacttcaac tacagctgcg
     1261 tgttcagcgt ggtcaatgcc accgtgaccg tgaacgtgac cacacacaat ggccgggtcg
     1321 tgaactacac cgtgtgcgac gattgcaacg gctacaccga caacatcttc agcgtgcagc
     1381 aggacggcag aatccccaat ggcttcccct tcaacaactg gttcctgctg accaacggca
     1441 gcaccctggt tgatggcgtg tccagactgt accagcctct gagactgacc tgcctgtggc
     1501 ctgtgcctgg actgaagtct agcaccggct tcgtgtactt caacgccacc ggcagcgacg
     1561 tgaactgcaa tggctaccag cacaacagcg tggtggacgt gatgcggtac aacctgaact
     1621 tctccgccaa cagcctggac aatctgaagt ccggcgtgat cgtgttcaag accctccagt
     1681 acgacgtgct gttctactgc tccaacagca gcagcggcgt gctggacacc accattcctt
     1741 ttggccctag cagccagcct tactactgct tcatcaactc caccatcaac accacacacg
     1801 tgtccacctt cgtgggcatc ctgcctccaa ccgtgcggga aattgtggtg gctagaaccg
     1861 gccagtttta catcaacggg ttcaagtact tcgacctggg cttcatcgag gccgtgaact
     1921 ttaatgtgac caccgccagc gccaccgatt tctggacagt ggcctttgcc acctttgtgg
     1981 acgtgctggt caatgtgtcc gccaccaaca tccagaatct gctgtactgc gacagcccct
     2041 tcgagaagct ccagtgcgaa cacctccagt tcggcctgca agacggcttc tactccgcca
     2101 atttcctgga cgacaacgtg ctgcccgaga catatgtggc cctgcctatc tactatcagc
     2161 acaccgacat caatttcacc gccacagcca gcttcggcgg cagctgttac gtgtgcaaac
     2221 cccaccaagt gaacatcagc ctgaacggca acacctccgt gtgtgtgcgg accagccact
     2281 tcagcatccg gtacatctac aacagagtga agtctggcag ccctggcgac agctcctggc
     2341 acatctacct gaaaagcggc acatgcccct tcagcttcag caagctgaac aacttccaga
     2401 agtttaagac catctgcttc agcaccgtgg aagtgcccgg cagctgcaac tttccactgg
     2461 aagccacctg gcactacacc agctacacaa tcgtgggcgc cctgtatgtg acttggagcg
     2521 agggcaatag catcaccggc gtgccatatc ctgtgtccgg catccgcgag ttcagcaatc
     2581 tggtgctgaa caattgcacc aagtacaata tctacgacta cgtcggcacc ggcatcatca
     2641 gaagcagcaa ccagtctctg gctggcggca tcacctacgt gtccaactct ggcaatctgc
     2701 tgggctttaa gaatgtgtcc accggcaata tcttcatcgt caccccttgc aaccagcctg
     2761 accaggtggc agtgtaccag cagtctatca tcggcgccat gaccgccgtg aacgagagca
     2821 gatatggcct ccagaacctc ctccagctgc ctaacttcta ctatgtgtcc aatggcggca
     2881 acaactgcac cacagccgtg atgacctaca gcaacttcgg catctgcgcc gacggcagcc
     2941 tgattcctgt cagacctaga aacagcagcg acaacggcat cagcgccatc atcaccgcta
     3001 acctgagcat ccccagcaac tggaccacaa gcgtgcaggt cgagtacctc cagatcacca
     3061 gcacacccat cgtggtcgat tgcgccacct atgtgtgcaa cggcaacccc agatgcaaaa
     3121 acctgctgaa acagtacacc agcgcctgca aaaccatcga ggacgccctg agactgtctg
     3181 cccacctgga aacaaacgac gtgtcctcca tgctgacctt cgacagcaat gccttcagcc
     3241 tggccaatgt gacctccttc ggcgactaca atctgagcag cgtgctgcca cagcggaaca
     3301 tcagatccag cagaatcgcc ggaagaagcg ccctcgagga tctgctgttt agcaaggtcg
     3361 tgacaagcgg cctgggcaca gtggacgtgg actacaagag ctgtaccaag ggcctgtcta
     3421 tcgccgatct ggcctgcgct cagtactata atggcatcat ggtgctcccc ggcgtggccg
     3481 atgctgaaag aatggccatg tacaccggaa gcctgatcgg cggaatggtg ctcggaggac
     3541 tgacttctgc cgccgctatt ccctttagcc tggctctcca ggccagactg aattacgtgg
     3601 ccctccagac cgatgtgctg caagagaacc agaagatcct ggccgccagc ttcaacaagg
     3661 ccatcaacaa catcgtggcc agcttctcca gcgtgaacga cgccattaca cagaccgccg
     3721 aggccatcca cacagtgaca atcgccctga acaagatcca ggacgtcgtg aaccagcagg
     3781 gcagcgccct gaatcacctg acaagccagc tgcggcacaa ctttcaggcc atcagcaatt
     3841 ccatccaggc catctacgac cggctggact ctatccaggc agatcagcag gtcgacagac
     3901 tgatcaccgg cagactggcc gctctgaacg cctttgtgtc ccaagtgctc aacaagtaca
     3961 ccgaagtgcg gggctctaga aggctggccc agcaaaagat caacgagtgc gtgaagtccc
     4021 agagcaacag atacggcttc tgcggcaacg gcacccacat cttctctatc gtgaacagcg
     4081 ccccagacgg cctcctgttt ctgcatacag tgctgctgcc caccgattac aagaacgtga
     4141 aggcttggag cggcatctgt gtggacggca tctacggcta tgtgctgaga cagcccaacc
     4201 tggtgctgta ctccgataac ggggtgttca gagtgaccag cagagtgatg ttccagccta
     4261 gactgcccgt gctgagcgac ttcgtgcaga tctacaactg taacgtgacc ttcgtgaaca
     4321 tctcccgcgt ggaactgcac actgtgatcc ccgattatgt ggatgtgaac aagaccctgc
     4381 aagagttcgc ccagaacctg cctaaatacg tgaagcccaa cttcgatctg acccctttca
     4441 acctgaccta cctgaatctg tccagcgaac tgaaacagct cgaggccaag accgccagcc
     4501 tgtttcagac cacagtggaa ctccagggcc tgatcgacca gatcaactct acctacgtgg
     4561 acctgaaact gctgaaccgc ttcgagaact acatcaagtg gccttggtgg gtctggctga
     4621 tcatctccgt ggtgttcgtg gtcctgctga gcctgctcgt gttctgctgt ctgtctaccg
     4681 gctgctgcgg ctgttgcaac tgtctgacca gctccatgag aggctgctgt gattgcggct
     4741 cctaactcga gccatggaag cttgatatct aactgactga accggtggta ccaaacgggg
     4801 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     4861 aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     4921 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     4981 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     5041 acgtcggggc ggcaggccct gccatagcag atctgcgcag ctggggctct agggggtatc
     5101 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5161 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5221 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5281 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     5341 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5401 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     5461 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5521 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     5581 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     5641 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5701 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5761 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     5821 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     5881 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
     5941 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     6001 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     6061 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     6121 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     6181 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     6241 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     6301 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     6361 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     6421 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     6481 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     6541 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     6601 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     6661 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     6721 tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca
     6781 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     6841 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     6901 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6961 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7021 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     7081 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7141 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7201 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7261 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7321 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7381 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7441 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7501 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7561 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7621 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7681 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7741 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7801 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7861 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7921 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7981 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8041 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8101 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8161 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8221 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8281 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8341 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8401 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8461 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8521 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8581 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8641 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8701 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8761 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8821 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8881 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8941 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9001 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9061 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9121 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9181 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     9241 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     9301 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     9361 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     9421 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gtt
//
