LOCUS       sequence-340349-        1146 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression/secretion in mammalian cells. CD44 splice variant
            10 (exons 2-5, 11, 15-16), A20-E649. Can be biotinylated..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Gileadi, et al
  TITLE     TREAT-AD plasmids 2021
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CD44U-c011
FEATURES             Location/Qualifiers
     source          1..1146
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1069..1113
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             1126..1143
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggggatcc ttcccagccc tgggatgcct gcgctgctct ccctcgtgag ccttctctcc
       61 gtgctgctga tgggttgcgt agctgaaacc ggtgcgcaga tcgatttgaa tataacctgc
      121 cgctttgcag gtgtattcca cgtggagaaa aatggtcgct acagcatctc tcggacggag
      181 gccgctgacc tctgcaaggc tttcaatagc accttgccca caatggccca gatggagaaa
      241 gctctgagca tcggatttga gacctgcagg tatgggttca tagaagggca tgtggtgatt
      301 ccccggatcc accccaactc catctgtgca gcaaacaaca caggggtgta catcctcaca
      361 tccaacacct cccagtatga cacatattgc ttcaatgctt cagctccacc tgaagaagat
      421 tgtacatcag tcacagacct gcccaatgcc tttgatggac caattaccat aactattgtt
      481 aaccgtgatg gcacccgcta tgtccagaaa ggagaataca gaacgaatcc tgaagacatc
      541 taccccagca accctactga tgatgacgtg agcagcggct cctccagtga aaggagcagc
      601 acttcaggag gttacatctt ttacaccttt tctactgtac accccatccc agacgaagac
      661 agtccctgga tcaccgacag cacagacaga atccctgcta ccaataggaa tgatgtcaca
      721 ggtggaagaa gagacccaaa tcattctgaa ggctcaacta ctttactgga aggttatacc
      781 tctcattacc cacacacgaa ggaaagcagg accttcatcc cagtgacctc agctaagact
      841 gggtcctttg gagttactgc agttactgtt ggagattcca actctaatgt caatcgttcc
      901 ttatcaggag accaagacac attccacccc agtggggggt cccataccac tcatggatct
      961 gaatcagatg gacactcaca tgggagtcaa gaaggtggag caaacacaac ctctggtcct
     1021 ataaggacac cccaaattcc agaaggtacc ggaggttccg gtggttccgg tctgaatgat
     1081 atctttgaag ctcagaagat tgaatggcat gaaggacgta ccaagcacca ccatcaccat
     1141 cactaa
//
