LOCUS       sequence-34035-e         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Taylor MJ, Perrais D, Merrifield CJ
  TITLE     A high precision survey of the molecular dynamics of Mammalian 
            clathrin-mediated endocytosis.
  JOURNAL   PLoS Biol. 2011 Mar . 9(3):e1000604.
  PUBMED    21445324
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Biol. 
            2011 Mar . 9(3):e1000604."
COMMENT     Sequence Label: Myo1E-pmAppleC1
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..751
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     457..476
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg gatagctagc accatggtga gcaagggcga
       61 ggagaataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
      121 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgaggcctt
      181 tcagaccgct aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
      241 gtcccctcag ttcatgtacg gctccaaggt ctacattaag cacccagccg acatccccga
      301 ctacttcaag ctgtccttcc ccgagggctt caggtgggag cgcgtgatga acttcgagga
      361 cggcggcatt attcacgtta accaggactc ctccctgcag gacggcgtgt tcatctacaa
      421 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
      481 gggctgggag gcctccgagg agcggatgta ccccgaggac ggcgccctga agagcgagat
      541 caagaagagg ctgaagctga aggacggcgg ccactacgcc gccgaggtca agaccaccta
      601 caaggccaag aagcccgtgc agctgcccgg cgcctacatc gtcgacatca agttggacat
      661 cgtgtcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
      721 ctccaccggc ggcatggacg agctgtacaa gtccggactc agatctcgag ctggacatgg
      781 tacaggatca actggtagtg gaagtagtat gggcagcaaa ggcgcctacc gataccattg
      841 gcagagccac aatgtcaagc acagtggtgt ggacgacatg gttctactgt ccaagatcac
      901 agagagctcc attgtagaga acctgaagaa gagatacatg gacgactata ttttttacat
      961 atata
//
