LOCUS       sequence-340353-        1398 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression in bacterial cells. Entire CDS,  M1-Y329..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Gileadi, et al
  TITLE     TREAT-AD plasmids 2021
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CNN3A-c002
FEATURES             Location/Qualifiers
     source          1..1398
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1333..1377
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             1378..1395
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgtgcagca gccatcatca tcatcatcac ggcagcggca gcggctctga ccaggaggca
       61 aaaccttcaa ctgaggactt gggggataag aaggaaggtg aatatattaa actcaaagtc
      121 attggacagg atagcagtga gattcacttc aaagtgaaaa tgacaacaca tctcaagaaa
      181 ctcaaagaat catactgtca aagacagggt gttccaatga attcactcag gtttctcttt
      241 gagggtcaga gaattgctga taatcatact ccaaaagaac tgggaatgga ggaagaagat
      301 gtgattgaag tttaccagga gcaaacggga ggtatgaccc acttcaacaa gggcccttcc
      361 tatgggctct cggccgaagt caagaacaag attgcttcca agtatgatca tcaggcagaa
      421 gaagatcttc gcaattggat agaagaggtg acaggcatga gcattggccc caacttccag
      481 ctgggcttaa aggatggcat catcctctgc gaacttataa acaagctaca gccaggctca
      541 gtgaagaagg tcaacgagtc ctcactgaac tggcctcagt tggagaatat tggcaacttt
      601 attaaagcta ttcaggctta tggtatgaag ccacatgaca tattcgaagc aaatgatctt
      661 tttgagaatg gaaacatgac ccaggttcag actactctgg tggctctagc aggtctggct
      721 aaaacaaaag gattccatac aaccattgac attggagtta agtatgcaga aaaacaaaca
      781 agacgttttg atgaaggaaa attaaaagct ggccaaagtg taattggtct gcagatggga
      841 accaacaaat gtgccagcca ggcaggtatg acagcttacg ggactaggag gcatctttat
      901 gatcccaaaa tgcaaactga caaacctttt gaccagacca caattagtct gcagatgggc
      961 actaataaag gagccagcca ggcagggatg ttagcaccag gtaccagaag agacatctat
     1021 gatcagaagc taacattaca gccggtggac aactcgacaa tttccctaca gatgggtacc
     1081 aacaaagttg cttcccagaa aggaatgagt gtgtatgggc ttgggcggca agtatatgat
     1141 cccaaatact gtgctgctcc tacagaacct gtcattcaca acggaagcca aggaacagga
     1201 acaaatggtt cggaaatcag tgatagtgat tatcaggcag aataccctga tgagtatcat
     1261 ggcgagtacc aggatgacta ccccagagat taccaatata gcgaccaagg cattgattat
     1321 tgaagcggtg gcggtctgaa cgacatcttc gaggctcaga aaatcgaatg gcacgaacac
     1381 caccaccacc accactga
//
