LOCUS       sequence-340355-        1203 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression in bacterial cells. Lacking N-terminal 10 
            residues, G11-Y329..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Gileadi, et al
  TITLE     TREAT-AD plasmids 2021
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CNN3A-c007
FEATURES             Location/Qualifiers
     source          1..1203
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1138..1182
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             1183..1200
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgtgcagca gccatcatca tcatcatcac ggcagcggca gcggctctga ccaggaggca
       61 aaaccttcaa ctgaggactt gggggataag aaggaaggtg aatatattaa actcaaagtc
      121 attggacagg atagcagtga gattcacttc aaagtgaaaa tgacaacaca tctcaagaaa
      181 ctcaaagaat catactgtca aagacagggt gttccaatga attcactcag gtttctcttt
      241 gagggtcaga gaattgctga taatcatact ccaaaagaac tgggaatgga ggaagaagat
      301 gtgattgaag tttaccagga gcaaacggga ggtgggctct cggccgaagt caagaacaag
      361 attgcttcca agtatgatca tcaggcagaa gaagatcttc gcaattggat agaagaggtg
      421 acaggcatga gcattggccc caacttccag ctgggcttaa aggatggcat catcctctgc
      481 gaacttataa acaagctaca gccaggctca gtgaagaagg tcaacgagtc ctcactgaac
      541 tggcctcagt tggagaatat tggcaacttt attaaagcta ttcaggctta tggtatgaag
      601 ccacatgaca tattcgaagc aaatgatctt tttgagaatg gaaacatgac ccaggttcag
      661 actactctgg tggctctagc aggtctggct aaaacaaaag gattccatac aaccattgac
      721 attggagtta agtatgcaga aaaacaaaca agacgttttg atgaaggaaa attaaaagct
      781 ggccaaagtg taattggtct gcagatggga accaacaaat gtgccagcca ggcaggtatg
      841 acagcttacg ggactaggag gcatctttat gatcccaaaa tgcaaactga caaacctttt
      901 gaccagacca caattagtct gcagatgggc actaataaag gagccagcca ggcagggatg
      961 ttagcaccag gtaccagaag agacatctat gatcagaagc taacattaca gccggtggac
     1021 aactcgacaa tttccctaca gatgggtacc aacaaagttg cttcccagaa aggaatgagt
     1081 gtgtatgggc ttgggcggca agtatatgat cccaaatact gtgcttgaag cggtggcggt
     1141 ctgaacgaca tcttcgaggc tcagaaaatc gaatggcacg aacaccacca ccaccaccac
     1201 tga
//
