LOCUS       sequence-340359-        1267 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression in bacterial cells. FERM domain,  M1-L390..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Gileadi, et al
  TITLE     TREAT-AD plasmids 2021
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: EPB41L3A-c001
FEATURES             Location/Qualifiers
     source          1..1267
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgacgaccga atctggatca gactcggaat ccaagccgga ccaggaggcc
      121 gagccccagg aggcggcggg ggcgcagggg cgcgcggggg cgcccgtgcc ggagccgccc
      181 aaggaggagc agcagcaggc cctggagcag ttcgccgccg ctgcagcgca cagcaccccg
      241 gtgcggaggg aggtcactga caaggaacag gagtttgctg ccagggctgc aaaacagctc
      301 gaatatcagc aattagaaga cgataaactt tctcagaaat catctagcag taaactctct
      361 cggtctccat taaagattgt caaaaagcct aaaagcatgc agtgcaaagt gatacttctc
      421 gatggatcag aatatacctg tgatgtagag aaacgctcca gaggacaagt gctgtttgat
      481 aaagtgtgtg aacacttgaa cttgctagag aaagactact ttgggcttac gtatcgagat
      541 gctgaaaacc agaagaattg gttggaccct gctaaggaaa taaaaaaaca ggttcgaagt
      601 ggtgcttggc acttttcatt taatgtgaaa ttttatccac cagaccctgc ccaactatct
      661 gaagatatca ccaggtacta cctctgcttg cagttgcgag atgacatcgt gtccggaagg
      721 ctgccctgct cctttgttac cctggccttg ctgggctcct acactgtcca gtcagagctc
      781 ggagactatg acccagatga atgtgggagc gattacatta gtgagttccg ctttgcacca
      841 aaccacacta aagaactgga agacaaagtg atcgagctgc acaagagcca cagaggaatg
      901 acgccagcag aagcagagat gcatttcttg gaaaatgcca aaaaattatc aatgtatggg
      961 gtagatttac atcatgctaa ggactcagaa ggggtagaaa ttatgttagg agtttgtgca
     1021 agtggtctgt tgatatatcg cgaccggctg cgaataaaca gatttgcctg gcccaaggtt
     1081 ctaaagattt catacaaacg gaacaacttt tacattaaga tccggccggg agagtttgaa
     1141 caatttgaaa gcaccattgg gtttaagctg ccaaaccatc gagctgccaa gcgtttatgg
     1201 aaagtatgtg ttgagcatca tacatttttc agactactgt gacagtaaag gtggatacgg
     1261 atccgaa
//
