LOCUS       sequence-340361-         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression in bacterial cells. FERM domain, P107-L390. Used 
            for crystallography (PDB:6IBE)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Gileadi, et al
  TITLE     TREAT-AD plasmids 2021
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: EPB41L3A-c021
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgcctaaaag catgcagtgc aaagtgatac ttctcgatgg atcagaatat
      121 acctgtgatg tagagaaacg ctccagagga caagtgctgt ttgataaagt gtgtgaacac
      181 ttgaacttgc tagagaaaga ctactttggg cttacgtatc gagatgctga aaaccagaag
      241 aattggttgg accctgctaa ggaaataaaa aaacaggttc gaagtggtgc ttggcacttt
      301 tcatttaatg tgaaatttta tccaccagac cctgcccaac tatctgaaga tatcaccagg
      361 tactacctct gcttgcagtt gcgagatgac atcgtgtccg gaaggctgcc ctgctccttt
      421 gttaccctgg ccttgctggg ctcctacact gtccagtcag agctcggaga ctatgaccca
      481 gatgaatgtg ggagcgatta cattagtgag ttccgctttg caccaaacca cactaaagaa
      541 ctggaagaca aagtgatcga gctgcacaag agccacagag gaatgacgcc agcagaagca
      601 gagatgcatt tcttggaaaa tgccaaaaaa ttatcaatgt atggggtaga tttacatcat
      661 gctaaggact cagaaggggt agaaattatg ttaggagttt gtgcaagtgg tctgttgata
      721 tatcgcgacc ggctgcgaat aaacagattt gcctggccca aggttctaaa gatttcatac
      781 aaacggaaca acttttacat taagatccgg ccgggagagt ttgaacaatt tgaaagcacc
      841 attgggttta agctgccaaa ccatcgagct gccaagcgtt tatggaaagt atgtgttgag
      901 catcatacat ttttcagact actgtgacag taaaggtgga tacggatccg aa
//
