LOCUS       sequence-340363-        1063 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression in bacterial cells. FERM domain,  M1-Q322..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Gileadi, et al
  TITLE     TREAT-AD plasmids 2021
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: EZRA-c004
FEATURES             Location/Qualifiers
     source          1..1063
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgccgaaacc aatcaatgtc cgagttacca ccatggatgc agagctggag
      121 tttgcaatcc agccaaatac aactggaaaa cagctttttg atcaggtggt aaagactatc
      181 ggcctccggg aagtgtggta ctttggcctc cactatgtgg ataataaagg atttcctacc
      241 tggctgaagc tggataagaa ggtgtctgcc caggaggtca ggaaggagaa tcccctccag
      301 ttcaagttcc gggccaagtt ctaccctgaa gatgtggctg aggagctcat ccaggacatc
      361 acccagaaac ttttcttcct ccaagtgaag gaaggaatcc ttagcgatga gatctactgc
      421 ccccctgaga ctgccgtgct cttggggtcc tacgctgtgc aggccaagtt tggggactac
      481 aacaaagaag tgcacaagtc tgggtacctc agctctgagc ggctgatccc tcaaagagtg
      541 atggaccagc acaaacttac cagggaccag tgggaggacc ggatccaggt gtggcatgcg
      601 gaacaccgtg ggatgctcaa agataatgct atgttggaat acctgaagat tgctcaggac
      661 ctggaaatgt atggaatcaa ctatttcgag ataaaaaaca agaaaggaac agacctttgg
      721 cttggagttg atgcccttgg actgaatatt tatgagaaag atgataagtt aaccccaaag
      781 attggctttc cttggagtga aatcaggaac atctctttca atgacaaaaa gtttgtcatt
      841 aaacccatcg acaagaaggc acctgacttt gtgttttatg ccccacgtct gagaatcaac
      901 aagcggatcc tgcagctctg catgggcaac catgagttgt atatgcgccg caggaagcct
      961 gacaccatcg aggtgcagca gatgaaggcc caggcccggg aggagaagca tcagaagcag
     1021 ctggagcggc aacagtgaca gtaaaggtgg atacggatcc gaa
//
