LOCUS       sequence-340367-         531 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression/secretion in mammalian cells. Entire CDS with 
            ectopic signal peptide, V21-D143.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Gileadi, et al
  TITLE     TREAT-AD plasmids 2021
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MDKA-c009
FEATURES             Location/Qualifiers
     source          1..531
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             499..516
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gaattcaagc ttgccaccat ggggatcctt cccagccctg ggatgcctgc gctgctctcc
       61 ctcgtgagcc ttctctccgt gctgctgatg ggttgcgtag ctgaaaccgg tgccgccgtc
      121 gccaaaaaga aagataaggt gaagaaaggt ggtcctggat cagaatgtgc tgaatgggca
      181 tggggaccat gtacaccaag cagcaaggat tgcggcgtgg gtttccgcga gggcacctgc
      241 ggggcccaga cccagcgcat ccggtgcagg gtgccctgca actggaagaa ggagtttgga
      301 gccgactgca agtacaagtt tgagaactgg ggtgcgtgtg atgggggcac aggcaccaaa
      361 gtccgccaag gcaccctgaa gaaggcgcgc tacaatgctc agtgccagga gaccatccgc
      421 gtcaccaagc cctgcacccc caagaccaaa gcaaaggcca aagccaagaa agggaaggga
      481 aaggacaaag gtaccaagca ccaccatcac catcactaat gatcactcga g
//
