LOCUS       sequence-340369-        1221 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression/secretion in mammalian cells. Entire CDS with 
            ectopic signal peptide, V21-D143. Fused to IgG Fc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Gileadi, et al
  TITLE     TREAT-AD plasmids 2021
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MDKA-c014
FEATURES             Location/Qualifiers
     source          1..1221
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             484..507
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             1189..1206
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gaattcaagc ttgccaccat ggggatcctt cccagccctg ggatgcctgc gctgctctcc
       61 ctcgtgagcc ttctctccgt gctgctgatg ggttgcgtag ctgaaaccgg tgtcgccaaa
      121 aagaaagata aggtgaagaa aggtggtcct ggatcagaat gtgctgaatg ggcatgggga
      181 ccatgtacac caagcagcaa ggattgcggc gtgggtttcc gcgagggcac ctgcggggcc
      241 cagacccagc gcatccggtg cagggtgccc tgcaactgga agaaggagtt tggagccgac
      301 tgcaagtaca agtttgagaa ctggggtgcg tgtgatgggg gcacaggcac caaagtccgc
      361 caaggcaccc tgaagaaggc gcgctacaat gctcagtgcc aggagaccat ccgcgtcacc
      421 aagccctgca cccccaagac caaagcaaag gccaaagcca agaaagggaa gggaaaggac
      481 aaactggaag ttctgtttca gggcccggac aaaactcaca catgcccacc gtgcccagca
      541 cctgaactcc tggggggacc gtcagtcttc ctcttccccc caaaacccaa ggacaccctc
      601 atgatctccc ggacccctga ggtcacatgc gtggtggtgg acgtgagcca cgaagaccct
      661 gaggtcaagt tcaactggta cgtggacggc gtggaggtgc ataatgccaa gacaaagccg
      721 cgggaggagc agtacaacag cacgtaccgt gtggtcagcg tcctcaccgt cctgcaccag
      781 gactggctga atggcaagga gtacaagtgc aaggtctcca acaaagccct cccagccccc
      841 atcgagaaaa ccatctccaa agccaaaggg cagccccgag aaccacaggt gtacaccctg
      901 cccccatccc gggaggagat gaccaagaac caggtcagcc tgacctgcct ggtcaaaggc
      961 ttctatccca gcgacatcgc cgtggagtgg gagagcaatg ggcagccgga gaacaactac
     1021 aagaccacgc ctcccgtgct ggactccgac ggctccttct tcctctacag caagctcacc
     1081 gtggacaaga gcaggtggca gcaggggaac gtcttctcat gctccgtgat gcacgaggct
     1141 ctgcacaacc actacacgca gaagagcctc tccctgtctc cgggtaaaca tcaccatcac
     1201 catcactaac tcgagactag t
//
