LOCUS       sequence-340371-        1828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression in bacterial cells. Entire CDS, M1-M577.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Gileadi, et al
  TITLE     TREAT-AD plasmids 2021
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MSNA-c000
FEATURES             Location/Qualifiers
     source          1..1828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgcccaaaac gatcagtgtg cgtgtgacca ccatggatgc agagctggag
      121 tttgccatcc agcccaacac caccgggaag cagctatttg accaggtggt gaaaactatt
      181 ggcttgaggg aagtttggtt ctttggtctg cagtaccagg acactaaagg tttctccacc
      241 tggctgaaac tcaataagaa ggtgactgcc caggatgtgc ggaaggaaag ccccctgctc
      301 tttaagttcc gtgccaagtt ctaccctgag gatgtgtccg aggaattgat tcaggacatc
      361 actcagcgcc tgttctttct gcaagtgaaa gagggcattc tcaatgatga tatttactgc
      421 ccgcctgaga ccgctgtgct gctggcctcg tatgctgtcc agtctaagta tggcgacttc
      481 aataaggaag tgcataagtc tggctacctg gccggagaca agttgctccc gcagagagtc
      541 ctggaacagc acaaactcaa caaggaccag tgggaggagc ggatccaggt gtggcatgag
      601 gaacaccgtg gcatgctcag ggaggatgct gtcctggaat atctgaagat tgctcaagat
      661 ctggagatgt atggtgtgaa ctacttcagc atcaagaaca agaaaggctc agagctgtgg
      721 ctgggggtgg atgccctggg tctcaacatc tatgagcaga atgacagact aactcccaag
      781 ataggcttcc cctggagtga aatcaggaac atctctttca atgataagaa atttgtcatc
      841 aagcccattg acaaaaaagc cccggacttc gtcttctatg ctccccggct gcggattaac
      901 aagcggatct tggccttgtg catggggaac catgaactat acatgcgccg tcgcaagcct
      961 gataccattg aggtgcagca gatgaaggca caggcccggg aggagaagca ccagaagcag
     1021 atggagcgtg ctatgctgga aaatgagaag aagaagcgtg aaatggcaga gaaggagaaa
     1081 gagaagattg aacgggagaa ggaggagctg atggagaggc tgaagcagat cgaggaacag
     1141 actaagaagg ctcagcaaga actggaagaa cagacccgta gggctctgga acttgagcag
     1201 gaacggaagc gtgcccagag cgaggctgaa aagctggcca aggagcgtca agaagctgaa
     1261 gaggccaagg aggccttgct gcaggcctcc cgggaccaga aaaagactca ggaacagctg
     1321 gccttggaaa tggcagagct gacagctcga atctcccagc tggagatggc ccgacagaag
     1381 aaggagagtg aggctgtgga gtggcagcag aaggcccaga tggtacagga agacttggag
     1441 aagacccgtg ctgagctgaa gactgccatg agtacacctc atgtggcaga gcctgctgag
     1501 aatgagcagg atgagcagga tgagaatggg gcagaggcta gtgctgacct acgggctgat
     1561 gctatggcca aggaccgcag tgaggaggaa cgtaccactg aggcagagaa gaatgagcgt
     1621 gtgcagaagc acctgaaggc cctcacttcg gagctggcca atgccagaga tgagtccaag
     1681 aagactgcca atgacatgat ccatgctgag aacatgcgac tgggccgaga caaatacaag
     1741 accctgcgcc agatccggca gggcaacacc aagcagcgca ttgacgaatt tgagtctatg
     1801 tgacagtaaa ggtggatacg gatccgaa
//
