LOCUS       sequence-340379-        1170 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression in bacterial cells. FERM domain, M1-E346. Can be 
            biotinylated..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Gileadi, et al
  TITLE     TREAT-AD plasmids 2021
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MSNA-c023
FEATURES             Location/Qualifiers
     source          1..1170
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1123..1167
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgc ccaaaacgat cagtgtgcgt gtgaccacca tggatgcaga gctggagttt
      121 gccatccagc ccaacaccac cgggaagcag ctatttgacc aggtggtgaa aactattggc
      181 ttgagggaag tttggttctt tggtctgcag taccaggaca ctaaaggttt ctccacctgg
      241 ctgaaactca ataagaaggt gactgcccag gatgtgcgga aggaaagccc cctgctcttt
      301 aagttccgtg ccaagttcta ccctgaggat gtgtccgagg aattgattca ggacatcact
      361 cagcgcctgt tctttctgca agtgaaagag ggcattctca atgatgatat ttactgcccg
      421 cctgagaccg ctgtgctgct ggcctcgtat gctgtccagt ctaagtatgg cgacttcaat
      481 aaggaagtgc ataagtctgg ctacctggcc ggagacaagt tgctcccgca gagagtcctg
      541 gaacagcaca aactcaacaa ggaccagtgg gaggagcgga tccaggtgtg gcatgaggaa
      601 caccgtggca tgctcaggga ggatgctgtc ctggaatatc tgaagattgc tcaagatctg
      661 gagatgtatg gtgtgaacta cttcagcatc aagaacaaga aaggctcaga gctgtggctg
      721 ggggtggatg ccctgggtct caacatctat gagcagaatg acagactaac tcccaagata
      781 ggcttcccct ggagtgaaat caggaacatc tctttcaatg ataagaaatt tgtcatcaag
      841 cccattgaca aaaaagcccc ggacttcgtc ttctatgctc cccggctgcg gattaacaag
      901 cggatcttgg ccttgtgcat ggggaaccat gaactataca tgcgccgtcg caagcctgat
      961 accattgagg tgcagcagat gaaggcacag gcccgggagg agaagcacca gaagcagatg
     1021 gagcgtgcta tgctggaaaa tgagaagaag aagcgtgaaa tggcagagaa ggagaaagag
     1081 aagattgaac gggagaagga ggagagcagt aaaggtggat acggcctgaa tgatatcttt
     1141 gaagcgcaga agattgaatg gcatgaatga
//
