LOCUS       sequence-340381-        1135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression in bacterial cells. FERM domain, M1-E346. 
            Contains H288A point mutation that reduces binding to CD44..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Du Y, Bradshaw WJ, Leisner TM, Annor-Gyamfi JK, Qian K, Bashore FM, 
            Sikdar A, Nwogbo FO, Ivanov AA, Frye SV, Gileadi O, Brennan PE, 
            Levey AI, Axtman AD, Pearce KH, Fu H, Katis VL
  TITLE     Discovery of FERM domain protein-protein interaction inhibitors for 
            MSN and CD44 as a potential therapeutic approach for Alzheimer's 
            disease.
  JOURNAL   J Biol Chem. 2023 Oct 20:105382. doi: 10.1016/j.jbc.2023.105382.
  PUBMED    37866628
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2023 Oct 20:105382. doi: 10.1016/j.jbc.2023.105382."
COMMENT     Sequence Label: MSNA-c028
FEATURES             Location/Qualifiers
     source          1..1135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgcccaaaac gatcagtgtg cgtgtgacca ccatggatgc agagctggag
      121 tttgccatcc agcccaacac caccgggaag cagctatttg accaggtggt gaaaactatt
      181 ggcttgaggg aagtttggtt ctttggtctg cagtaccagg acactaaagg tttctccacc
      241 tggctgaaac tcaataagaa ggtgactgcc caggatgtgc ggaaggaaag ccccctgctc
      301 tttaagttcc gtgccaagtt ctaccctgag gatgtgtccg aggaattgat tcaggacatc
      361 actcagcgcc tgttctttct gcaagtgaaa gagggcattc tcaatgatga tatttactgc
      421 ccgcctgaga ccgctgtgct gctggcctcg tatgctgtcc agtctaagta tggcgacttc
      481 aataaggaag tgcataagtc tggctacctg gccggagaca agttgctccc gcagagagtc
      541 ctggaacagc acaaactcaa caaggaccag tgggaggagc ggatccaggt gtggcatgag
      601 gaacaccgtg gcatgctcag ggaggatgct gtcctggaat atctgaagat tgctcaagat
      661 ctggagatgt atggtgtgaa ctacttcagc atcaagaaca agaaaggctc agagctgtgg
      721 ctgggggtgg atgccctggg tctcaacatc tatgagcaga atgacagact aactcccaag
      781 ataggcttcc cctggagtga aatcaggaac atctctttca atgataagaa atttgtcatc
      841 aagcccattg acaaaaaagc cccggacttc gtcttctatg ctccccggct gcggattaac
      901 aagcggatct tggccttgtg catggggaac gctgaactat acatgcgccg tcgcaagcct
      961 gataccattg aggtgcagca gatgaaggca caggcccggg aggagaagca ccagaagcag
     1021 atggagcgtg ctatgctgga aaatgagaag aagaagcgtg aaatggcaga gaaggagaaa
     1081 gagaagattg aacgggagaa ggaggagtga cagtaaaggt ggatacggat ccgaa
//
