LOCUS       sequence-340385-        1101 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression in bacterial cells. FERM domain, M1-R338..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Gileadi, et al
  TITLE     TREAT-AD plasmids 2021
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: NF2A-c001
FEATURES             Location/Qualifiers
     source          1..1101
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggccggggc catcgcttcc cgcatgagct tcagctctct caagaggaag
      121 caacccaaga cgttcaccgt gaggatcgtc accatggacg ccgagatgga gttcaattgc
      181 gagatgaagt ggaaagggaa ggacctcttt gatttggtgt gccggactct ggggctccga
      241 gaaacctggt tctttggact gcagtacaca atcaaggaca cagtggcctg gctcaaaata
      301 gacaagaagg tactggatca tgatgtttca aaggaagaac cagtcacctt tcacttcttg
      361 gccaaatttt atcctgagaa tgctgaagag gagctggttc aggagatcac acaacattta
      421 ttcttcttac aggtaaagaa gcagatttta gatgaaaaga tctactgccc tcctgaggct
      481 tctgtgctcc tggcttctta cgccgtccag gccaagtatg gtgactacga ccccagtgtt
      541 cacaagcggg gatttttggc ccaagaggaa ttgcttccaa aaagggtaat aaatctgtat
      601 cagatgactc cggaaatgtg ggaggagaga attactgctt ggtacgcaga gcaccgaggc
      661 cgagccaggg atgaagctga aatggaatat ctgaagatag ctcaggacct ggagatgtac
      721 ggtgtgaact actttgcaat ccggaataaa aagggcacag agctgctgct tggagtggat
      781 gccctggggc ttcacattta tgaccctgag aacagactga cccccaagat ctccttcccg
      841 tggaatgaaa tccgaaacat ctcgtacagt gacaaggagt ttactattaa accactggat
      901 aagaaaattg atgtcttcaa gtttaactcc tcaaagcttc gtgttaataa gctgattctc
      961 cagctatgta tcgggaacca tgatctattt atgaggagaa ggaaagccga ttctttggaa
     1021 gttcagcaga tgaaagccca ggccagggag gagaaggcta gaaagcagat ggagcggcag
     1081 cgctgacagt aaaggtggat a
//
