LOCUS       sequence-340387-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression in bacterial cells. Entire CDS, M1-K199. Can be 
            biotinylated..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Gileadi, et al
  TITLE     TREAT-AD plasmids 2021
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PRDX1A-c001
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             682..726
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgt cttcaggaaa tgctaaaatt gggcaccctg cccccaactt caaagccaca
      121 gctgttatgc cagatggtca gtttaaagat atcagcctgt ctgactacaa aggaaaatat
      181 gttgtgttct tcttttaccc tcttgacttc acctttgtgt gccccacgga gatcattgct
      241 ttcagtgata gggcagaaga atttaagaaa ctcaactgcc aagtgattgg tgcttctgtg
      301 gattctcact tctgtcatct agcatgggtc aatacaccta agaaacaagg aggactggga
      361 cccatgaaca ttcctttggt atcagacccg aagcgcacca ttgctcagga ttatggggtc
      421 ttaaaggctg atgaaggcat ctcgttcagg ggccttttta tcattgatga taagggtatt
      481 cttcggcaga tcactgtaaa tgacctccct gttggccgct ctgtggatga gactttgaga
      541 ctagttcagg ccttccagtt cactgacaaa catggggaag tgtgcccagc tggctggaaa
      601 cctggcagtg ataccatcaa gcctgatgtc caaaagagca aagaatattt ctccaagcag
      661 aagagcagta aaggtggata cggcctgaat gatatctttg aagcgcagaa gattgaatgg
      721 catgaatga
//
