LOCUS       sequence-340391-         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression in bacterial cells. Entire CDS, M1-N215. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Gileadi, et al
  TITLE     TREAT-AD plasmids 2021
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RAB5AA-c003
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggctagtcg aggcgcaaca agacccaacg ggccaaatac tggaaataaa
      121 atatgccagt tcaaactagt acttctggga gagtccgctg ttggcaaatc aagcctagtg
      181 cttcgttttg tgaaaggcca atttcatgaa tttcaagaga gtaccattgg ggctgctttt
      241 ctaacccaaa ctgtatgtct tgatgacact acagtaaagt ttgaaatatg ggatacagct
      301 ggtcaagaac gataccatag cctagcacca atgtactaca gaggagcaca agcagccata
      361 gttgtatatg atatcacaaa tgaggagtcc tttgcaagag caaaaaattg ggttaaagaa
      421 cttcagaggc aagcaagtcc taacattgta atagctttat cgggaaacaa ggccgaccta
      481 gcaaataaaa gagcagtaga tttccaggaa gcacagtcct atgcagatga caatagttta
      541 ttattcatgg agacatccgc taaaacatca atgaatgtaa atgaaatatt catggcaata
      601 gctaaaaaat tgccaaagaa tgaaccacaa aatccaggag caaattctgc cagaggaaga
      661 ggagtagacc ttaccgaacc cacacaacca accaggaatc agtgttgtag taactgacag
      721 taaaggtgga tacggatccg aa
//
