LOCUS       sequence-340393-         610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression in bacterial cells. GTPase domain, G15-N184. Can 
            be used for crystallography..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Gileadi, et al
  TITLE     TREAT-AD plasmids 2021
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RAB5AA-c006
FEATURES             Location/Qualifiers
     source          1..610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgggaaataa aatatgccag ttcaaactag tacttctggg agagtccgct
      121 gttggcaaat caagcctagt gcttcgtttt gtgaaaggcc aatttcatga atttcaagag
      181 agtaccattg gggctgcttt tctaacccaa actgtatgtc ttgatgacac tacagtaaag
      241 tttgaaatat gggatacagc tggtcaagaa cgataccata gcctagcacc aatgtactac
      301 agaggagcac aagcagccat agttgtatat gatatcacaa atgaggagtc ctttgcaaga
      361 gcaaaaaatt gggttaaaga acttcagagg caagcaagtc ctaacattgt aatagcttta
      421 tcgggaaaca aggccgacct agcaaataaa agagcagtag atttccagga agcacagtcc
      481 tatgcagatg acaatagttt attattcatg gagacatccg ctaaaacatc aatgaatgta
      541 aatgaaatat tcatggcaat agctaaaaaa ttgccaaaga attgacagta aaggtggata
      601 cggatccgaa
//
