LOCUS       sequence-340415-        1236 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression in bacterial cells. Coiled-coil domains, 
            M1-L275..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Gileadi, et al
  TITLE     TREAT-AD plasmids 2021
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: STX4AA-c005
FEATURES             Location/Qualifiers
     source          1..1236
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1171..1215
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             1216..1233
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgtgcagca gccatcatca tcatcatcac ggcagcggca gcggctctga ccaggaggca
       61 aaaccttcaa ctgaggactt gggggataag aaggaaggtg aatatattaa actcaaagtc
      121 attggacagg atagcagtga gattcacttc aaagtgaaaa tgacaacaca tctcaagaaa
      181 ctcaaagaat catactgtca aagacagggt gttccaatga attcactcag gtttctcttt
      241 gagggtcaga gaattgctga taatcatact ccaaaagaac tgggaatgga ggaagaagat
      301 gtgattgaag tttaccagga gcaaacggga ggtatgcggg acaggaccca cgagctgaga
      361 cagggggatg acagctcgga cgaagaggac aaggagcggg tcgcgctggt ggtgcacccg
      421 ggcacggcac ggctggggag cccggacgag gagttcttcc acaaggtccg gacaattcgg
      481 cagactattg tcaaactggg gaataaagtc caggagttgg agaaacagca ggtcaccatc
      541 ctggccacgc cccttcccga ggagagcatg aagcaggagc tgcagaacct gcgcgatgag
      601 atcaaacagc tggggaggga gatccgcctg cagctgaagg ccatagagcc ccagaaggag
      661 gaagctgatg agaactataa ctccgtcaac acaagaatga gaaaaaccca gcatggggtc
      721 ctgtcccagc aattcgtgga gctcatcaac aagtgcaatt caatgcagtc cgaataccgg
      781 gagaagaacg tggagcggat tcggaggcag ctgaagatca ccaatgctgg gatggtgtct
      841 gatgaggagt tggagcagat gctggacagt gggcaaagcg aggtgtttgt gtccaatatc
      901 ctgaaggaca cgcaggtgac tcgacaggcc ttaaatgaga tctcggcccg gcacagtgag
      961 atccagcagc ttgaacgcag tattcgtgag ctgcacgaca tattcacttt tctggctacc
     1021 gaagtggaga tgcaggggga gatgatcaat cggattgaga agaacatcct gagctcagcg
     1081 gactacgtgg aacgtgggca ggagcacgtc aagacggccc tggagaacca gaagaaggcg
     1141 aggaagaaga aagtcttgtg aagcggtggc ggtctgaacg acatcttcga ggctcagaaa
     1201 atcgaatggc acgaacacca ccaccaccac cactga
//
