LOCUS       sequence-340417-        2151 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression in insect cells. Entire CDS, M1-N635. Codon 
            optimised..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Gileadi, et al
  TITLE     TREAT-AD plasmids 2021
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SYKA-c014
FEATURES             Location/Qualifiers
     source          1..2151
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..210
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     CDS             217..237
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ggatccatgg gacatcacca tcatcaccac tcaagcgggg ttgacaataa attcaacaag
       61 gagcgacgtc gggcacgccg tgaaatacga cacctcccta atctcaatcg agaacaacgg
      121 cgtgcgttta taaggagtct gcgtgacgac cctagccaat cagctaactt gttggctgaa
      181 gccaagaaac ttaacgatgc tcaacccaaa ggcacggaga atttgtattt ccagtcgatg
      241 gcatcctctg gaatggcaga ttctgccaat cacctaccct ttttcttcgg caatataact
      301 agagaggaag cagaggatta ccttgtgcag ggtgggatgt cggacggact ctacctgctt
      361 cgccaatcga gaaactacct aggcgggttc gccctgtcag tggcccatgg tagaaaggcg
      421 caccactata cgatcgagag ggagctcaac ggaacctacg ctatcgcggg tggccggact
      481 catgcgagcc cggctgattt atgtcattac catagtcaag agagcgatgg attggtctgt
      541 ttattgaaga aacctttcaa ccgccctcag ggcgtgcaac cgaaaaccgg gccctttgag
      601 gaccttaagg aaaacctcat acgtgagtac gtaaagcaga cttggaactt gcaagggcag
      661 gcactagagc aagcgattat ttcccagaag cctcaactag aaaaactgat tgcaaccacg
      721 gcacatgaaa aaatgccttg gttccatggg aaaatctccc gagaagaatc agaacaaata
      781 gttttaattg ggtcgaaaac aaacggcaag tttctcattc gggctaggga taataatggg
      841 agttacgccc tgtgtcttct gcatgaggga aaggtcttac actaccgcat cgataaagat
      901 aaaaccggga agttatcaat cccagaaggc aaaaaattcg atactctatg gcagttagtc
      961 gagcactact catacaaggc tgatggactc ctgcgggtat tgacagttcc gtgccaaaag
     1021 atcggcacac agggaaacgt gaattttgga ggtcggcccc agttaccagg ttcccatcca
     1081 gcgacctgga gcgcaggagg gataatatcg agaatcaaat cctattcctt tccgaagcca
     1141 ggtcaccgaa aatcgtctcc cgctcaaggg aacaggcaag aatctacagt cagtttcaac
     1201 ccatacgagc ccgagctggc gccctgggcc gcggataaag gcccgcagcg tgaggcattg
     1261 cccatggaca ccgaggtgta cgaatctccc tatgctgatc ctgaggagat tagaccaaag
     1321 gaagtctacc ttgaccggaa gctactgaca ttggaggaca aagaactcgg tagtggaaat
     1381 tttggtacgg taaaaaaggg ctattaccag atgaagaagg tggttaaaac ggttgctgta
     1441 aaaatcctaa agaacgaagc caacgaccca gcccttaagg acgaactgct agctgaagcg
     1501 aatgtgatgc agcagctaga taatccttat atagtaagaa tgatcggaat atgtgaggcc
     1561 gaaagttgga tgctagtaat ggagatggcg gaattgggac ctctcaataa atatcttcaa
     1621 cagaacaggc atgtaaaaga caaaaatatc attgaattgg tgcaccaggt gtctatgggg
     1681 atgaaatacc ttgaagagtc aaacttcgta catcgcgatc tagcagcccg aaatgtcctc
     1741 ctggttacgc aacactatgc caagatatcg gatttcggtc ttagcaaggc attaagggca
     1801 gatgaaaatt attataaggc tcaaactcac gggaaatggc cggttaagtg gtatgcgcca
     1861 gagtgcatta attactataa gttttcgagc aagagcgacg tttggagttt tggtgtcctc
     1921 atgtgggaag ctttttccta tggccagaag ccttaccgcg gaatgaaagg ttctgaggta
     1981 actgcgatgt tagaaaaagg cgagcgtatg ggctgtccgg ctggttgccc gcgtgaaatg
     2041 tatgacttga tgaatctttg ctggacttat gatgtcgaga acaggccagg ttttgcagcc
     2101 gtggaactgc gcttaagaaa ctactattat gacgttgtta attgactcga g
//
