LOCUS       sequence-340419-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. 
            Can be used for crystallography..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Bashore FM, Katis VL, Du Y, Sikdar A, Wang D, Bradshaw WJ, Rygiel 
            KA, Leisner TM, Chalk R, Mishra S, Williams AC, Gileadi O, Brennan 
            PE, Wiley JC, Gockley J, Cary GA, Carter GW, Young JE, Pearce KH, Fu
            H, Axtman AD
  TITLE     Characterization of covalent inhibitors that disrupt the interaction
            between the tandem SH2 domains of SYK and FCER1G phospho-ITAM.
  JOURNAL   bioRxiv. 2023 Jul 29:2023.07.28.551026. doi: 
            10.1101/2023.07.28.551026. Preprint.
  PUBMED    37547005
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 Jul 29:2023.07.28.551026. doi: 10.1101/2023.07.28.551026. 
            Preprint."
COMMENT     Sequence Label: SYKA-c020
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggctgacag cgccaaccac ctgcccttct ttttcggcaa catcacccgg
      121 gaggaggcag aagattacct ggtccagggg ggcatgagtg atgggcttta tttgctgcgc
      181 cagagccgca actacctggg tggcttcgcc ctgtccgtgg cccacgggag gaaggcacac
      241 cactacacca tcgagcggga gctgaatggc acctacgcca tcgccggtgg caggacccat
      301 gccagccccg ccgacctctg ccactaccac tcccaggagt ctgatggcct ggtctgcctc
      361 ctcaagaagc ccttcaaccg gccccaaggg gtgcagccca agactgggcc ctttgaggat
      421 ttgaaggaaa acctcatcag ggaatatgtg aagcagacat ggaacctgca gggtcaggct
      481 ctggagcagg ccatcatcag tcagaagcct cagctggaga agctgatcgc taccacagcc
      541 catgaaaaaa tgccttggtt ccatggaaaa atctctcggg aagaatctga gcaaattgtc
      601 ctgataggat caaagacaaa tggaaagttc ctgatccgag ccagagacaa caacggctcc
      661 tacgccctgt gcctgctgca cgaagggaag gtgctgcact atcgcatcga caaagacaag
      721 acagggaagc tctccatccc cgagggaaag aagttcgaca cgctctggca gctagtcgag
      781 cattattctt ataaagcaga tggtttgtta agagttctta ctgtcccatg tcaaaaaatc
      841 ggcacacagg gaaatgttaa ttgacagtaa aggtggata
//
