LOCUS       sequence-340421-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. 
            Can be biotinylated..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Bashore FM, Katis VL, Du Y, Sikdar A, Wang D, Bradshaw WJ, Rygiel 
            KA, Leisner TM, Chalk R, Mishra S, Williams AC, Gileadi O, Brennan 
            PE, Wiley JC, Gockley J, Cary GA, Carter GW, Young JE, Pearce KH, Fu
            H, Axtman AD
  TITLE     Characterization of covalent inhibitors that disrupt the interaction
            between the tandem SH2 domains of SYK and FCER1G phospho-ITAM.
  JOURNAL   bioRxiv. 2023 Jul 29:2023.07.28.551026. doi: 
            10.1101/2023.07.28.551026. Preprint.
  PUBMED    37547005
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 Jul 29:2023.07.28.551026. doi: 10.1101/2023.07.28.551026. 
            Preprint."
COMMENT     Sequence Label: SYKA-c022
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             877..921
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg ctgacagcgc caaccacctg cccttctttt tcggcaacat cacccgggag
      121 gaggcagaag attacctggt ccaggggggc atgagtgatg ggctttattt gctgcgccag
      181 agccgcaact acctgggtgg cttcgccctg tccgtggccc acgggaggaa ggcacaccac
      241 tacaccatcg agcgggagct gaatggcacc tacgccatcg ccggtggcag gacccatgcc
      301 agccccgccg acctctgcca ctaccactcc caggagtctg atggcctggt ctgcctcctc
      361 aagaagccct tcaaccggcc ccaaggggtg cagcccaaga ctgggccctt tgaggatttg
      421 aaggaaaacc tcatcaggga atatgtgaag cagacatgga acctgcaggg tcaggctctg
      481 gagcaggcca tcatcagtca gaagcctcag ctggagaagc tgatcgctac cacagcccat
      541 gaaaaaatgc cttggttcca tggaaaaatc tctcgggaag aatctgagca aattgtcctg
      601 ataggatcaa agacaaatgg aaagttcctg atccgagcca gagacaacaa cggctcctac
      661 gccctgtgcc tgctgcacga agggaaggtg ctgcactatc gcatcgacaa agacaagaca
      721 gggaagctct ccatccccga gggaaagaag ttcgacacgc tctggcagct agtcgagcat
      781 tattcttata aagcagatgg tttgttaaga gttcttactg tcccatgtca aaaaatcggc
      841 acacagggaa atgttaatag cagtaaaggt ggatacggcc tgaatgatat ctttgaagcg
      901 cagaagattg aatggcatga atga
//
