LOCUS       sequence-340425-        2808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression in bacterial cells. Entire CDS, M1-L700 (CAPN2); 
            EF hand 1-5, H86-S268 (CAPNS1). CAPN2 can be biotinylated. Codon 
            optimised..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Gileadi, et al
  TITLE     TREAT-AD plasmids 2021
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: XX01CAPN2A-c001
FEATURES             Location/Qualifiers
     source          1..2808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..60
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2182..2226
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcg agaatcttta ttttcagggc
       61 catatggctg gaatagcggc aaagctagct aaagatcgtg aggctgcgga aggtctgggt
      121 tctcatgatc gtgcgatcaa atacctgaac caggactacg aagctttgag aaacgagtgc
      181 ctggaagctg gtacattgtt tcaggatccg tccttcccgg caatcccgag cgcgctgggc
      241 tttaaggagc tgggcccata cagcagcaaa accagaggta ttgaatggaa gcgcccaact
      301 gaaatttgcg ctgatccgca atttattatt ggcggtgcca cccgcaccga tatctgccaa
      361 ggtgcgcttg gggattgttg gctgttggca gccatcgcaa gtttgaccct gaacgaggag
      421 atcctggctc gtgtggtgcc gctgaaccag tccttccaag aaaattacgc ggggatattt
      481 cactttcagt tttggcagta tggcgaatgg gttgaggtcg ttgtggacga ccggctgccg
      541 acgaaggacg gtgaactgtt attcgtgcat tccgcggagg gctctgagtt ctggtcagcc
      601 cttctggaaa aagcgtacgc gaaaatcaat ggctgctatg aggcactgag cggtggtgcg
      661 accaccgagg gcttcgagga cttcacgggt ggcatagcgg agtggtatga actcaagaag
      721 ccaccgccta atctttttaa gatcatccaa aaagcgttac aaaagggctc cctgctcggg
      781 tgcagcattg acatcactag cgcggcggac agcgaagcga ttaccttcca aaagttggtt
      841 aaaggtcatg catacagcgt caccggtgct gaggaggtgg aaagcaatgg ttctctgcaa
      901 aagctgatcc gaattcgtaa tccgtggggt gaagtggaat ggactggtcg ttggaacgat
      961 aactgcccga gctggaacac cattgacccg gaagaacgcg agcgtttaac ccgtcgtcac
     1021 gaagacggcg agttctggat gtcgttcagc gacttcctgc gtcattatag ccgcctagag
     1081 atctgcaacc tgactccgga taccctcacg agcgacacct ataaaaaatg gaagttgacg
     1141 aagatggatg gcaattggcg tcgtggctct acggccggcg gctgccgtaa ctaccccaac
     1201 accttttgga tgaacccgca gtacctgatt aagttagaag aagaagacga ggacgaagaa
     1261 gacggcgagt ccggctgcac cttcctggtc ggtctgatcc aaaagcaccg tcgtcgccag
     1321 cgtaaaatgg gtgaagacat gcacaccatc ggcttcggta tttatgaggt gccggaagag
     1381 ttgagtggtc agaccaatat tcatctctct aagaacttct ttttgacgaa ccgcgcgaga
     1441 gagcgcagcg ataccttcat caatttgcgt gaggtcttga accgctttaa gctaccgccg
     1501 ggtgagtata ttctggttcc gtcgaccttc gaaccgaata aagacggtga tttttgtatc
     1561 cgtgtatttt ctgaaaaaaa ggcggattat caggcagttg atgacgagat cgaggctaat
     1621 ctggaggagt tcgatatcag cgaggacgat attgacgacg gtttccgccg cctgtttgca
     1681 cagctggcgg gtgaggatgc agagattagc gcgtttgagc tgcaaactat cctgcgccgt
     1741 gttctggcca aacgtcagga cattaaatcc gatggcttct ccatcgaaac ctgtaaaatc
     1801 atggttgaca tgctggatag cgatggcagc ggtaagctgg gcctcaaaga attctatatc
     1861 ctgtggacca agattcaaaa gtaccagaaa atctaccgtg aaattgatgt tgatcgctcc
     1921 ggtacgatga acagctatga aatgcgcaaa gcgctggaag aggccggctt caaaatgccg
     1981 tgtcagcttc accaggttat cgtggcgcgt tttgccgatg accagctgat tatcgacttc
     2041 gacaactttg ttcgttgcct ggtgcgtttg gagaccctgt ttaaaatttt caagcagttg
     2101 gacccggaga acaccggcac catcgagctg gacctgatta gctggctgtg tttttcggtg
     2161 ctgggctcta agggtggtta cggtttgaac gatattttcg aagctcaaaa aattgaatgg
     2221 cacgagtaat aataatttaa ggagatatac atgagtcact attcaaacat agaagctaat
     2281 gagagcgaag aggtgcgcca gtttagacgt ttattcgccc aattggcggg cgatgatatg
     2341 gaagtcagcg caaccgaact gatgaacatc ctgaataaag tagttacccg tcatccggat
     2401 ttgaaaaccg acggcttcgg tattgatacg tgccgttcta tggttgctgt gatggacagc
     2461 gatactacgg gtaaactggg tttcgaggag ttcaagtacc tgtggaataa catcaaacgt
     2521 tggcaggcga tttataagca gttcgacacc gatcgttccg gcaccatctg ttcctcggaa
     2581 ctgccgggtg cgtttgaagc ggcgggcttc cacttgaacg agcacctgta taacatgatt
     2641 atccgtcgct actctgacga aagcggtaat atggattttg acaactttat cagctgcctg
     2701 gttcgtctgg acgctatgtt ccgcgcattt aagtccctcg acaaggacgg caccggtcag
     2761 attcaagtga acatccaaga gtggctgcag ttgaccatgt acagctaa
//
