LOCUS       sequence-340434-        2878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vitro transcription of B18R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2878)
  TITLE     Verstreken lab plasmids
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIVTR(T7A)-B18R
FEATURES             Location/Qualifiers
     source          1..2878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1123..1711)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1203..1222)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1882..2673,2674..2742))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2505..2524
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2743..2847)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taatacgact cactataagg acatttgctt ctgacacaac tgtgttcact agcaacctca
       61 aacagaattc gagctcggta cccggggatc ctctagagtc gccaccatga caatgaaaat
      121 gatggtccac atttattttg tctctcttag tctgctcctc ttgctttttc atagctatgc
      181 gatcgacata gaaaacgaaa taacggagtt ttttaataaa atgcgagaca ccctgcctgc
      241 aaaagattcc aagtggctca acccagcgtg catgttcggt ggcacgatga atgatatcgc
      301 ggccttgggc gagccctttt ctgccaaatg tcctccaata gaggactccc ttctgtccca
      361 ccggtataaa gactatgttg ttaaatggga gaggttggag aaaaacagac gaaggcaggt
      421 aagtaacaaa agagtaaagc acggggatct ctggattgcc aactacacct caaaattctc
      481 aaatcggcgg tatctctgta ctgtgacgac caagaatggt gattgcgtgc agggtatcgt
      541 cagaagccac atcagaaaac ctccctcctg cattccgaaa acttatgagc ttggaactca
      601 cgataagtat gggatcgact tgtattgtgg catcctttat gccaaacatt acaacaatat
      661 aacatggtat aaggataaca aagagattaa tattgatgac atcaaatatt ctcaaaccgg
      721 gaagaaactg atcatccata acccagaact ggaggactca ggtcgctacg actgctatgt
      781 tcattacgat gacgtaagga ttaagaatga tatcgttgtc agtcgctgta agatactgac
      841 cgtgctgcct tctcaggacc ataggttcaa attgaagcga aattgtgggt atgcctccaa
      901 ttgagacctg caggcatgca agcttgctcg ctttcttgct gtccaatttc tattaaaggt
      961 tcctttgttc cctaagtcca actactaaac tgggggatat tatgaagggc cttgagcatc
     1021 tggattctgc ctaataaaaa acatttattt tcattgcaaa accatgggag caaaaggcca
     1081 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1141 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1201 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1261 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1321 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1381 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1441 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1501 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1561 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1621 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1681 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1741 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     1801 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1861 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     1921 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     1981 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     2041 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     2101 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     2161 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     2221 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     2281 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     2341 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     2401 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     2461 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     2521 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     2581 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     2641 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     2701 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     2761 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     2821 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
