LOCUS       sequence-340446-        2347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vitro transcription of K3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2347)
  TITLE     Verstreken lab plasmids
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIVTR(T7A)-K3
FEATURES             Location/Qualifiers
     source          1..2347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(125..713)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(205..224)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(884..1675,1676..1744))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1507..1526
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1745..1849)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attatgaagg gccttgagca tctggattct gcctaataaa aaacatttat tttcattgca
       61 aaaccatggg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      121 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      181 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      241 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      301 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
      361 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
      421 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
      481 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
      541 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
      601 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
      661 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
      721 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
      781 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
      841 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
      901 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
      961 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     1021 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     1081 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     1141 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     1201 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     1261 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     1321 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     1381 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     1441 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     1501 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     1561 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     1621 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     1681 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     1741 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     1801 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     1861 gaaaagtgcc acctgacgtc taatacgact cactataagg acatttgctt ctgacacaac
     1921 tgtgttcact agcaacctca aacagaattc gagctcggta cccggggatc ctctagagtc
     1981 gccaccatgc ttgccttctg ctactctctt cctaatgcag gagatgtgat aaagggaagg
     2041 gtttacgaaa aagactacgc gctctatatc tacttgtttg actatccaca tagtgaagct
     2101 attttggcag agtcagtcaa gatgcatatg gatcgctatg tcgagtatcg cgataaattg
     2161 gtcgggaaga cagtcaaagt caaggttatc cgggtagact atactaaggg atatatagat
     2221 gtgaactaca agagaatgtg tagacaccag tgagacctgc aggcatgcaa gcttgctcgc
     2281 tttcttgctg tccaatttct attaaaggtt cctttgttcc ctaagtccaa ctactaaact
     2341 gggggat
//
