LOCUS       sequence-340448-        2938 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vitro transcription of TDsmURFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2938)
  TITLE     Verstreken lab plasmids
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIVTR(AG)-TDsmURFP
FEATURES             Location/Qualifiers
     source          1..2938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             385..402
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     rep_origin      complement(609..1197)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(689..708)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1368..2159,2160..2228))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1991..2010
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2229..2333)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgtgtaaaca tcgcaactct gctgactgag aacaaaaaga aaatcgttga taaggcgtct
       61 caggatctgt ggcgtcgtca tccagatctc attgcgccgg gcggcatcgc ttttagtcaa
      121 cgcgatcgtg cactgtgcct gcgtgactac ggctggtttc tgcacctgat cacgttctgt
      181 ctgctggctg gtgataaagg tcctattgaa tccatcggtc taattagcat acgcgaaatg
      241 tacaacagcc tgggtgtgcc ggttccggct atgatggaat ctatccgttg tctgaaagag
      301 gcctccctgt cactgctgga cgaggaagac gccaatgaga ctgcaccgta ctttgactat
      361 atcatcaagg ctatgtccga attccatcat caccatcacc attgaaagct tgctcgcttt
      421 cttgctgtcc aatttctatt aaaggttcct ttgttcccta agtccaacta ctaaactggg
      481 ggatattatg aagggccttg agcatctgga ttctgcctaa taaaaaacat ttattttcat
      541 tgcaaaacca tgggagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      601 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      661 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      721 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      781 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      841 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      901 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      961 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1021 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1081 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1141 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1201 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1261 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1321 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     1381 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1441 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     1501 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     1561 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     1621 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     1681 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     1741 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     1801 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     1861 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     1921 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     1981 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2041 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2101 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2161 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2221 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2281 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     2341 ccccgaaaag tgccacctga cgtctaatac gactcactat aaggacattt gcttctgaca
     2401 caactgtgtt cactagcaac ctcaaacaga attcgagctc ggtacccggg gatccatgaa
     2461 aacttctgaa caacgtgtaa acatcgcaac tctgctgact gagaacaaaa agaaaatcgt
     2521 tgataaggcg tctcaggatc tgtggcgtcg tcatccagat ctcattgcgc cgggcggcat
     2581 cgcttttagt caacgcgatc gtgcactgtg cctgcgtgac tacggctggt ttctgcacct
     2641 gatcacgttc tgtctgctgg ctggtgataa aggtcctatt gaatccatcg gtctaattag
     2701 catacgcgaa atgtacaaca gcctgggtgt gccggttccg gctatgatgg aatctatccg
     2761 ttgtctgaaa gaggcctccc tgtcactgct ggacgaggaa gacgccaatg agactgcacc
     2821 gtactttgac tatatcatca aggctatgtc cgggcatggc accggcagca ccggcagcgg
     2881 cagctccggc accgcctcct ccgaggacaa caacatggcc atgaaaactt ctgaacaa
//
