LOCUS       sequence-340455-        2886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vitro transcription of NGN2 (Ser phosphosites substituted by 
            Ala).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2886)
  TITLE     Verstreken lab plasmids
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIVTR(T7A)-NGN2 (SA Substitutions)
FEATURES             Location/Qualifiers
     source          1..2886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     375..394
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1636..1740
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1741..1809,1810..2601)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1959..1978)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(2772..2886,1..474)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
       61 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      121 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      181 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      241 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
      301 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      361 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      421 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      481 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctcc catggttttg
      541 caatgaaaat aaatgttttt tattaggcag aatccagatg ctcaaggccc ttcataatat
      601 cccccagttt agtagttgga cttagggaac aaaggaacct ttaatagaaa ttggacagca
      661 agaaagcgag caagcttagc tctagataca atccctggct atggggaggt gaggtgcata
      721 gcggtgcttg tcgggaggtg ggggctgcca atagtccatg tctgacccgg ccggagcggc
      781 gggagctaaa gtgcagctgt agggagcggt ggaattggag gacacggagg aggacggcgc
      841 gggagcgttg gtgcaactcc acgtggaggc gggagcggga gcgtctccgc tgctgctcag
      901 ggcggcgctg gctcctcccg gagccagcaa cactgcctca gaaaagagcg ctcccggcag
      961 tccccctccg ccgcctccgc agtgatctgc caggcgcagt gtctcggtga gtgcccagat
     1021 gtagttgtgg gcgaagcgca gggtctcgat cttggtgagc ttggcgtcct cggggaacgt
     1081 ggggagcacc tcgcgcagcg cgtccagtgc cgcgttgagg ttgtgcatgc ggtttcgctc
     1141 gcggttgttg gccttcagtc tacgggtctt cttgatgcgc tgcaccgtct cggccgtctt
     1201 ggcgcctcgg gagacggccc gcgcccggga agggcgccgt ttgcaatcgt gtaccagacc
     1261 cagcagccgt gcgggccggc agccctccgc acccgcagcc acgccgcctc tcgctccctg
     1321 tccggcttca gcccctctct gtctacgcgc tccgcctgaa gctccaggct cctcctcctc
     1381 ttcttcgtcg gcgctggatg acagcggggt cagggccgcc aaggcgggag cggccgatcc
     1441 gagcagcact aacacgtcct cttcctcctt caactccaag gtctcggatt tgacgaacat
     1501 cttagttggc tctggatccc cgggtaccga gctcgaattc tgtttgaggt tgctagtgaa
     1561 cacagttgtg tcagaagcaa atgtccttat agtgagtcgt attagacgtc aggtggcact
     1621 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     1681 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     1741 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     1801 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     1861 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     1921 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     1981 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     2041 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     2101 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     2161 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     2221 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     2281 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     2341 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     2401 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     2461 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     2521 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     2581 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     2641 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     2701 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     2761 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2821 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2881 gtaact
//
