LOCUS       sequence-340491-       14397 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes OptoINVRT7 circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14397)
  AUTHORS   Zhao EM, Lalwani MA, Lovelett RJ, Garcia-Echauri SA, Hoffman SM, 
            Gonzalez CL, Toettcher JE, Kevrekidis IG, Avalos JL
  TITLE     Design and Characterization of Rapid Optogenetic Circuits for 
            Dynamic Control in Yeast Metabolic Engineering.
  JOURNAL   ACS Synth Biol. 2020 Dec 18;9(12):3254-3266. doi: 
            10.1021/acssynbio.0c00305. Epub 2020 Nov 24.
  PUBMED    33232598
REFERENCE   2  (bases 1 to 14397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00305"; journalName: "ACS Synth Biol"; date: 
            "2020-12-18- 18"; volume: "9"; issue: "12"; pages: "3254-3266"
COMMENT     Sequence Label: EZ-L439
FEATURES             Location/Qualifiers
     source          1..14397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(336..501)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     422..446
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(1075..3717)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             complement(4919..6223)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL80"
                     /product="transcriptional repressor that inhibits GAL4"
                     /label=GAL80
                     /note="GAL80 binds to and inhibits the transcriptional 
                     activation domain of GAL4."
                     /translation="MDYNKRSSVSTVPNAAPIRVGFVGLNAAKGWAIKTHYPAILQLSS
                     QFQITALYSPKIETSIATIQRLKLSNATAFPTLESFASSSTIDMIVIAIQVASHYDVVM
                     PLLEFSKNNPNLKYLFVEWALACSLDQAESIYKAAAERGVQTIISLQGRKSPYILRAKE
                     LISQGYIGDINSIEIAGNGGWYGYERPVKSPKYIYEIGNGVDLVTTTFGHTIDILQYMT
                     SSYFSRINAMVFNNIPEQELIDERGNRLGQRVPKTVPDHLLFQGTLLNGNVPVSCSFKG
                     GKPTKKFTKNLVIDIHGTKGDLKLEGDAGFAEISNLVLYYSGTRANDFPLANGQQAPLD
                     PGYDAGKEIMEVYHLRNYNAIVGNIHRLYQSISDFHFNTKKIPELPSQFVMQGFDFEGF
                     PTLMDALILHRLIESVYKSNMMGSTLNVSNISHYSL"
     promoter        complement(6280..6311)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     promoter        complement(7902..8089)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     promoter        complement(8415..8433)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8454..8470)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8454..8470)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8467..8489)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8478..8494
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8502..8508,8509..8526,8527..8532))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8547..8568
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8685..8702)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8856..9444)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8936..8955)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9615..10406,10407..10475))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10238..10257
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10476..10580)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10664..10682
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10720..10742)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10842..10861
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(11217..11672)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11354..11375)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11566..11585
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11798..11820
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11812..11829
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11813..11829
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11836..11855
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11836..11854
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        12518..12915
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             12945..12965
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      13854..14101
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(13870..13888)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        14281..14312
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
ORIGIN
        1 tgtgcgcgta tgtttatgta tgtacctctc tctctatttc tatttttaaa ccaccctctc
       61 aataaaataa aaataataaa gtatttttaa ggaaaagacg tgtttaagca ctgactttat
      121 ctactttttg tacgttttca ttgatataat gtgttttgtc tctccctttt ctacgaaaat
      181 ttcaaaaatt gaccaaaaaa aggaatatat atacgaaaaa ctattatatt tatatatcat
      241 agtgttgata aaaaatgttt atccattgga ccgtgtatca tatttatcgc cggcgaataa
      301 attaaggcgc gccttaattt attcgccggc gataagagcg acctcatgct atacctgaga
      361 aagcaacctg acctacagga aagagttact caagaataag aattttcgtt ttaaaaccta
      421 agagtcactt taaaatttgt atacacttat tttttttata acttatttaa taataaaaat
      481 cataaatcat aagaaattcg ctgatcatta ttattggaag tacaagtttt cagaaccagc
      541 cttcttgtac aaagaagtaa tagattcttg tgcacaagac ataggaagtt ctcgaaccgc
      601 ttcatcgata ttcacagctg tttttttcac caggtcttct ccctctttca ctgcggtttc
      661 ttcaatgaca ttgcgaactg gttctacgtg cttgctcccg tctagttgaa ctccaataaa
      721 gtattgtact tctcccttct gatcacgcat aggttgcaag tggaaaatgt tccagaactt
      781 ctttccgctc ttggtgtagt tgatgagctg aactgtcact tcggtttggt tatcaatagc
      841 atttcgaatc ttcttcactg tggttagatc agtctctgga ccttgtagaa acctgcaatt
      901 tcttccaaga atttcttcac ggctatattc cgtgagctcc aagaaactat cagatgcaaa
      961 aataatggga ttatcgggaa gcctaggatc agtgataata aaattcttct cgatacgttc
     1021 gagtgtagta gctagatcaa taccctttct catctgtcct ccagcactct cgagctcttt
     1081 ttttgggttt ggtggggtat cttcatcatc gaatagatag ttatatacat catccattgt
     1141 agtggtatta aacatccctg tagtgattcc aaacgcgtta tacgcagttt ggtccgtcca
     1201 accaggtgac agtggttttg aattattacc atcatcaatt ttactagccg tgatttcatt
     1261 attcatgaag ttatcatgaa cgttagagga ggcaattggt tgtgaaagcg cttgagaatt
     1321 tgtttgagtt gttatgaggt tcggaccgtt gctactgtta gtgaaagtga aggacaatga
     1381 gctatcagca atattcccac tttgattaaa attggcgcca ccaaacaaag cagacggggt
     1441 cagtggcact aatgattgca gctgttgctg ttgccctaga aaaggcgtga ctgagcgatg
     1501 cgaaggtgtg cttcttggta ttgtcactgg agagttacga gagggtggac ggttagataa
     1561 cagcttgact agatcactga aacttgctcc tgatttcaat ggcacaggtg aaggccctac
     1621 tgagccagga gaaacatatt taacactgat attgttgaca ttttcctccg gaagagtagg
     1681 gtattgggcg atagttgcag aaccgacaat atttttaatg gcgctaccat tactattgtt
     1741 ataactgata tgcggtaatg ggattgcaca ctgtgataac agaaacggcg cacatacctc
     1801 ttccagtact tgaatgtatt tttcacaagt ctggatttta aaagtggcca gtttttttaa
     1861 tagcatcaga acagtgttaa tttgttgtaa taattgtgcg gtctcgttat tctcagcatt
     1921 cgattttgag tttgagagta gagtctttat gggtactagg actgcattga acaagtaata
     1981 agaacaattc caggcaaaat atggggtgac attatgattg tccatatagc tacttacaga
     2041 cataacagtt ctttgtgctg catcgcttaa catgatggag catcgtttaa cttcataact
     2101 ttgatgatca ttttgatcct gttctagttg tgactttttc tgggtaaaat tagtgaaaaa
     2161 atctcttaat acataaatga taagagacaa ctgtttccac ttcagttcga atcttgtaaa
     2221 ggatagccaa gggtgttcct tcaacaaatt ggttagagcg gtggtggaaa tatccatttg
     2281 taaaaacttt ggtgcctgtc tcgaaacctc ctcaatctca ttacaaatca tcaagcattt
     2341 ttttgcacat ataggacttt tttctgcagt tactgttttg tctagttcat agatttttgt
     2401 gaaaacttgt aagagccttg ctgtttcaat gatgccatga tatatggtgg gacctgttgt
     2461 ggtacgctgc acatcgtcga cagaagaagg gaaggagatt gtattctgag aaagctggat
     2521 ggatcgacca taaagcaggg acaattggat ctcccaagag tagacagacc accaaattcg
     2581 gcgtctttgt tccagaatgc tgctatcact gaaggacgag gggaggtccc tattcaagcc
     2641 caatgatatg gccattctta tggaaaagct gtgaaaatta tagctagtat ttgttttctg
     2701 cctccactgt gtatatcgcg acagaagatg tagggctgtc accaaaatta tggaacctga
     2761 ctcgaagacc ttgctcgtca aatgagattt agcattttga tagtaaaaaa catctatatc
     2821 agtagattcc ccctctatac accaggctcc aatggctaat atgcagttaa aaaggatttg
     2881 ccattgatcc ttcgacgcga tttcaatctg gttattatac aacatcatta gcgtcggtga
     2941 gtgcacgata gggcagtagg ggtgaaaatt attgagataa ctttgaagta aacgggatgt
     3001 tgtggatcta gaagccaacg tgtatctatc cgtaatcatg gtcgggagcc tgttaacgtt
     3061 agagttcgtg taattttccg gtttaaagcc aatagatcga agaatacata agagagaacc
     3121 gtcgccaaag aacccattat tgttggggtc cgttttcagg aagggcaagc catccgacat
     3181 gtcatcctct tcagaccaat caaatccatg aagagcatcc ctgggcataa aatccaacgg
     3241 aattgtggag ttatcatgat gagctgccga gtcaatcgat acagtcaact gtctttgacc
     3301 tttgttacta ctctcttccg atgatgatgt cgcacttatt ctatgctgtc tcaatgttag
     3361 aggcatatca gtctccactg aagccaatct atctgtgacg gcatctttat tcacattatc
     3421 ttgtacaaat aatcctgtta acaatgcttt tatatcctgt aaagaatcca ttttcaaaat
     3481 catgtcaagg tcttctcgag gaaaaatcag tagaaatagc tgttccagtc tttctagcct
     3541 tgattccact tctgtcagat gtgccctagt cagcggagac cttttggttt tgggagagta
     3601 gcgacactcc cagttgttct tcagacactt ggcgcacttc ggtttttctt tggagcactt
     3661 gagcttttta agtcggcaaa tatcgcatgc ttgttcgata gaagacagta gcttcatgct
     3721 agccatgatt ttagcggccg ccaccgcggt gttttatatt tgttgtaaaa agtagataat
     3781 tacttccttg atgatctgta aaaaagagaa aaagaaagca tctaagaact tgaaaaacta
     3841 cgaattagaa aagaccaaat atgtatttct tgcattgacc aatttatgca agtttatata
     3901 tatgtaaatg taagtttcac gaggttctac taaactaaac cacccccttg gttagaagaa
     3961 aagagtgtgt gagaacaggc tgttgttgtc acacgattcg gacaattctg tttgaaagag
     4021 agagagtaac agtacgatcg aacgaacttt gctctggaga tcacagtggg catcatagca
     4081 tgtggtacta aaccctttcc cgccattcca gaaccttcga ttgcttgtta caaaacctgt
     4141 gagccgtcgc taggaccttg ttgtgtgacg aaattggaag ctgcaatcaa taggaagaca
     4201 ggaagtcgag cgtgtctggg ttttttcagt tttgttcttt ttgcaaacaa atcacgagcg
     4261 acggtaattt ctttctcgat aagaggccac gtgctttatg agggtaacat caattcaaga
     4321 aggagggaaa cacttccttt ttctggccct gataatagta tgagggtgaa gccaaaataa
     4381 aggattcgcg cccaaatcgg catctttaaa tgcaggtatg cgatagttcc tcactctttc
     4441 cttactcacg agtaattctt gcagctgttt cccggcgata aatatgatac acggtccaat
     4501 ggataaacat tttttatcaa cactatgata tataaatata atagtttttc gtatatatat
     4561 tccttttttt ggtcaatttt tgaaattttc gtagaaaagg gagagacaaa acacattata
     4621 tcaatgaaaa cgtacaaaaa gtagataaag tcagtgctta aacacgtctt ttccttaaaa
     4681 atactttatt atttttattt tattgagagg gtggtttaaa aatagaaata gagagagagg
     4741 tacatacata aacatacgcg cacaaaagca gagatgatca ttatacgttt atcctggctg
     4801 aagcacatgc ggccctttct ggacaagaag ctgggtgtct atccatccct gattcctggg
     4861 cacaagacat aggtaatgtc ccgtcatctt gctcttcaac ttcgggggga aactcgagta
     4921 aactataatg cgagatattg ctaacgttta atgtggagcc catcatgtta cttttataaa
     4981 cgctctcgat taacctgtgt aatatcagag catccatcaa ggtgggaaag ccttcgaaat
     5041 cgaaaccttg cattacaaat tgtgagggta attcaggaat tttctttgta ttgaagtgga
     5101 agtcagagat agattgatac agtcgatgaa tattaccgac aatggcatta taatttcgta
     5161 aatgatatac ttccatgatt tctttacctg catcataccc cgggtctaaa ggagcttgtt
     5221 gtccattagc tagcgggaag tcgtttgctc tagttccact gtagtaaagg accagatttg
     5281 aaatttctgc gaatccggca tcgccttcaa gtttcaaatc tcccttggta ccgtgaatat
     5341 caatgaccaa atttttggta aattttttgg taggtttgcc acctttgaaa ctgcatgaca
     5401 ctggaacatt gccatttaac aatgtgcctt ggaataaaag atgatccggt actgtctttg
     5461 ggactcgctg gcccaatcgg ttaccacgct catctatcag ctcttgctct ggaatattat
     5521 tgaaaaccat tgcatttatc ctggaaaagt acgaacttgt catgtattgt aaaatatcga
     5581 ttgtgtgacc aaatgttgtg gttaccagat ctacaccgtt cccgatttca tagatgtatt
     5641 ttggtgattt aacaggcctt tcgtagccgt accaaccgcc atttccagca atctcgatag
     5701 aattaatgtc gccgatatag ccttgagata ttaattcttt tgctctcaaa atatatggtg
     5761 atttacgacc ttgtaaagag atgatggttt gaaccccacg ttcagcagca gccttataaa
     5821 tggattcggc ttgatctagt gaacatgcaa gggcccattc tacgaaaaga tacttgaggt
     5881 tcggattatt tttggagaat tccaagagag gcataacaac gtcataatga ctggccactt
     5941 ggatagctat cactatcata tctatagtgg aagatgatgc aaatgactct aaagtgggaa
     6001 aagcagtggc attactcaat tttagacgct ggatggtggc aatagaagtc tcaatttttg
     6061 gactgtataa ggcagtgatt tgaaattgtg acgatagttg cagtatggcg gggtaatgtg
     6121 tcttgattgc ccatcctttg gctgcgttga gaccgacgaa tccgactctt atgggagctg
     6181 cattaggcac ggttgagacc gaagatctct tgttgtagtc catgctagcc atgattttag
     6241 cggccgcggt ggctttacca acagtaccgg attgccaagc tggaagtcga gcttccatta
     6301 tataccctct agtgtctctt aagcatggac taaaggctac ctataacgca tggactaaag
     6361 gctacctata acgcatggac taaaggctac ctataacgca tggactaaag gctacctata
     6421 acgcatggac taaaggctac ctactcgaag ctggcgaact cacgtacccg ggtataccgg
     6481 aggtacccaa agccgaatcc accacggtca ccgttgtttc tgaagaaacc accacggccg
     6541 ccaccgttgt tgaaaccacc acgaggagca gaatcgcctc ttctgccacc accgttacgg
     6601 atgtgaggtg gaacgtaacc accacgtctt tgttgccctt caggcatgga aacttgctct
     6661 gctaactcag tcatattgga tatatgtatt actttcttta ttttttgtag tttagtcttc
     6721 tatttctttt atagtttggt atctttttgc aaaaaattta cctctatatc gtgttcgtgc
     6781 gtatttcttc ctctataata tgaaaagaaa ggcagtagtc ttggttttct attattccaa
     6841 atattacaag aatacccaga accacaagaa gcagaagaaa ctaactgctt tatatactta
     6901 atttcccgcc acaagctcat cgcccagaag tttttttcag tttttcacct gacaatctgg
     6961 cagctcgctg aaaaattttc acctggggtc gcctgctacg tagagtgatc acgtttatga
     7021 atagcacatt atatagtggc ccagacggtc tgatggtagt tcatgtagtt aagaataaat
     7081 actctgtttg tgtgatttct atgtgtacgt tatctatata aatattgttg atggtggtag
     7141 ctgtgggctg tgttctatgc taggacaccc ttagtggatg gaacgtcatc gcgacccgta
     7201 ggtgttcttg cctctctgat ggcgatggcg agcgccttga aagctgattc ggagcggtgg
     7261 tggtcgttgg tgccccgcag acagtccaca tgcatggtga tacgcgcctc agtggcgaaa
     7321 ctctccaaga agtgcgggat catctctgtg gatagctggc ctatgcgttc tcgcttcagg
     7381 cccagctcca ccacggcgaa aggcctattg gagaagtcaa ccacagcgcg gctcagcgcc
     7441 tcgtccagtg gtgcaaaccc atgcccgaac ctcttgatac cacgcacgga gccaagcgcc
     7501 tccttgaacg cttggcccag cgcaatccca cagtcctcaa cagtgtggtg gtcatcgatg
     7561 tgcaagtccc ctatacactc caggatcaga gaccacccgc catgcttcgc cagcgcagtc
     7621 aacatgtggt ccaaaaaccc aactccagtg tgcacatgga tggtctgccc atcaccacta
     7681 gcatattcct tgcccagtat gctctcccgt acagaaacag acccaccgtc aagatccagc
     7741 gccagctgta tattcgtctc ctgcgtaacc ctcttaacaa acgccatcct ttgctcgatg
     7801 cttctctttg cttcaattat ctcaacttta agtaaacctg aagtaaacta gaactagatt
     7861 aaatcagaag gcatccgcag ctttatatac atatagagcc tctttgcctt cgtttatctt
     7921 gcctgctcat tttttagtat attcttcgaa gaaatcacat tactttatat aatgtataat
     7981 tcattatgtg ataatgccaa tcgctaagaa aaaaaaagag tcatccgcta ggtggaaaaa
     8041 aaaaaatgaa aatcattacc gaggcataaa aaaatataga gtgtactaga ggaggccaag
     8101 agtaatagaa aaagaaaatt gcgggaaagg actgtgttat gacttccctg actaatgccg
     8161 tgttcaaacg atacctggca gtgactccta gcgctcacca agctcttaaa acgagaatta
     8221 agaaaaagtc gtcatctttc gataagtttt tcccacagca aagcaatagt agaaaaaaac
     8281 aatgggaaac gttgaatgaa gacaaagcgt cgtggtttaa aaggaaatac gctcacgtac
     8341 atgctaggga acaggaccgt gcagcggcca gcgtggccgt ttaaacattt aaatggccgg
     8401 cccagctttt gttcccttta gtgagggtta attccgagct tggcgtaatc atggtcatag
     8461 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     8521 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8581 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     8641 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8701 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8761 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8821 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8881 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8941 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9001 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9061 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9121 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9181 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9241 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     9301 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9361 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9421 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9481 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9541 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9601 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9661 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9721 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9781 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9841 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9901 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9961 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    10021 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    10081 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10141 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    10201 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10261 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10321 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10381 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10441 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    10501 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    10561 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cctcggcgcg
    10621 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
    10681 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
    10741 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
    10801 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
    10861 actgagagtg caccatacca ccttttcaat tcatcatttt ttttttattc ttttttttga
    10921 tttcggtttc cttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg
    10981 aaggagcaca gacttagatt ggtatatata cgcatattgg gtcagcagta cagaaccctc
    11041 gacgaagttg taagtggtgg atcagatatc atcattgttg gcagaggact tttcgccaag
    11101 ggtagagatc ctaatactaa actcacaaat tagagcttca atttaattat atcagttatt
    11161 accctatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
    11221 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
    11281 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
    11341 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
    11401 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
    11461 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
    11521 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
    11581 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
    11641 cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt
    11701 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
    11761 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
    11821 acggccagtg aattgtaata cgactcacta tagggggcca gcgtggccgt ttaaacattt
    11881 aaatggccgg ccgctgccac cgttgtttcc accgaagaaa ccaccgttgc cgtaaccacc
    11941 acgacggttg ttgctaaaga agctgccacc gccacggcca ccgttgtagc cgccgttgtt
    12001 gttattgtag ttgctactgt tatttctggc acttcttggt tttcctctta agtgaggagg
    12061 aacataacca ttctcgttgt tgtcgttgat gcttaaattt tgcacttgtt cgctcagttc
    12121 agccataata tgaaatgctt ttcttgttgt tcttacggaa taccacttgc cacctatcac
    12181 cacaactaac tttttcccgt tcctccatct cttttatatt ttttttctgg agttcaagag
    12241 aaaaaaaaag aaaaagcaaa aagaaaaaag gaaagcgcgc ctcgttcaga atgacacgta
    12301 tagaatgatg cattaccttg tcatcttcag tatcatactg ttcgtataca tacttactga
    12361 cattcatagg tatacatata tacacatgta tatatatcgt atgctgcagc tttaaataat
    12421 cggtgtcaag atctgagctc caccgcggtg gcggccgctt atttactcag gatcccgcat
    12481 gttgcaggtg ggagttccag ctcccgggaa acagctgata gcttcaaaat gtttctactc
    12541 cttttttact cttccagatt ttctcggact ccgcgcatcg ccgtaccact tcaaaacacc
    12601 caagcacagc atactaaatt tcccctcttt cttcctctag ggtgtcgtta attacccgta
    12661 ctaaaggttt ggaaaagaaa aaagagaccg cctcgtttct ttttcttcgt cgaaaaaggc
    12721 aataaaaatt tttatcacgt ttctttttct tgaaaatttt ttttttgatt tttttctctt
    12781 tcgatgacct cccattgata tttaagttaa taaacggtct tcaatttctc aagtttcagt
    12841 ttcatttttc ttgttctatt acaacttttt ttacttcttg ctcattagaa agaaagcata
    12901 gcaatctaat ctaagttttg gcggccgcta aaatcatggc tagcccgaaa aaaaagagaa
    12961 aagtagcccc accaacggac gtttcacttg gtgacgaatt acatttagac ggtgaggatg
    13021 tagctatggc acatgccgat gctcttgatg attttgatct ggacatgtta ggtgatgggg
    13081 attctcccgg ccccggattt acaccacatg attctgctcc gtatggcgcg ttggacatgg
    13141 cagatttcga atttgaacag atgtttactg atgcacttgg tatcgacgaa tatggaggtg
    13201 aattcggagc agatgatact agagttgaag tccaacctcc agcccaatgg gtattggatt
    13261 tgatcgaggc ctcacccata gcaagtgttg ttagtgatcc cagattggct gataatcctt
    13321 tgattgctat caaccaggcc tttaccgatc taactgggta ttctgaggaa gaatgtgttg
    13381 gtcgtaattg tagatttttg gcaggatctg gtactgaacc ctggctaaca gataagatcc
    13441 gtcaaggtgt tcgtgagcat aaacccgtgt tggttgagat tttgaattac aaaaaggatg
    13501 gtactccttt tcgtaatgca gttttggtgg caccaatcta tgatgacgat gatgaattac
    13561 tatacttcct tggtagccaa gtggaagtgg atgacgatca accgaacatg ggcatggccc
    13621 gtagagaacg tgctgcggag atgttaaaaa ctctttcacc acgtcaatta gaagtcacaa
    13681 ctctggttgc atcaggccta agaaataaag aagtggctgc cagactgggt ctttcagaaa
    13741 aaactgtcaa aatgcacaga ggtttggtaa tggaaaaatt aaacttaaaa acgagtgcag
    13801 atttagttag aattgccgta gaagcaggta tttaagtcga cctcgagtaa tgatcatgta
    13861 attagttatg tcacgcttac attcacgccc tccccccaca tccgctctaa ccgaaaagga
    13921 aggagttaga caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt
    13981 aagaacgtta tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg
    14041 taacattata ctgaaaacct tgcttgagaa ggttttggga cgctcgaagg ctttaatttg
    14101 cggccttatc gccggtatac ccgggtacgt gagttcgcca gcttcgagta ggtagccttt
    14161 agtccatgcg ttataggtag cctttagtcc atgcgttata ggtagccttt agtccatgcg
    14221 ttataggtag cctttagtcc atgcgttata ggtagccttt agtccatgct taagagacac
    14281 tagagggtat ataatggaag ctcgacttcc agcttggcaa tccggtactg ttggtaaagc
    14341 caccgcggcc gctaaaatca tggctagcat ggactacaac aagagatctt cggtctc
//
