LOCUS       sequence-340511-       18325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes OptoQ-INVRT4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18325)
  AUTHORS   Lalwani MA, Zhao EM, Wegner SA, Avalos JL
  TITLE     The Neurospora crassa Inducible Q System Enables Simultaneous 
            Optogenetic Amplification and Inversion in Saccharomyces cerevisiae 
            for Bidirectional Control of Gene Expression.
  JOURNAL   ACS Synth Biol. 2021 Aug 20;10(8):2060-2075. doi: 
            10.1021/acssynbio.1c00229. Epub 2021 Aug 4.
  PUBMED    34346207
REFERENCE   2  (bases 1 to 18325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00229"; journalName: "ACS Synth Biol"; date: 
            "2021-08-20- 20"; volume: "10"; issue: "8"; pages: "2060-2075"
COMMENT     Sequence Label: pMAL498
FEATURES             Location/Qualifiers
     source          1..18325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        314..501
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     promoter        2083..2114
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     terminator      complement(8013..8178)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     8099..8123
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(8194..8901)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(8234..8254)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     8849..8877
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     promoter        complement(12425..12456)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     terminator      complement(12627..12874)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     12840..12858
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(13763..13783)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(13813..14210)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     complement(14873..14892)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(14874..14892)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(14899..14916)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(14899..14915)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14908..14930)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      15056..15511
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15143..15162)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15353..15374
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(15867..15886)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15986..16008
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(16046..16064)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        16148..16252
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(16253..16321,16322..17113)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(16471..16490)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      17284..17872
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     17773..17792
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     18026..18043
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    18160..18181
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(18196..18201,18202..18219,18220..18226)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    18234..18250
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     18239..18261
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     18258..18274
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     18258..18274
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        18295..18313
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccgct gcacggtcct gttccctagc
       61 atgtacgtga gcgtatttcc ttttaaacca cgacgctttg tcttcattca acgtttccca
      121 ttgttttttt ctactattgc tttgctgtgg gaaaaactta tcgaaagatg acgacttttt
      181 cttaattctc gttttaagag cttggtgagc gctaggagtc actgccaggt atcgtttgaa
      241 cacggcatta gtcagggaag tcataacaca gtcctttccc gcaattttct ttttctatta
      301 ctcttggcct cctctagtac actctatatt tttttatgcc tcggtaatga ttttcatttt
      361 tttttttcca cctagcggat gactcttttt ttttcttagc gattggcatt atcacataat
      421 gaattataca ttatataaag taatgtgatt tcttcgaaga atatactaaa aaatgagcag
      481 gcaagataaa cgaaggcaaa gaggctctat atgtatataa agctgcggat gccttctgat
      541 ttaatctagt tctagtttac ttcaggttta cttaaagttg agataattga agcaaagaga
      601 agcatcgagc aaaggatggc gtttgttaag agggttacgc aggagacgaa tatacagctg
      661 gcgctggatc ttgacggtgg gtctgtttct gtacgggaga gcatactggg caaggaatat
      721 gctagtggtg atgggcagac catccatgtg cacactggag ttgggttttt ggaccacatg
      781 ttgactgcgc tggcgaagca tggcgggtgg tctctgatcc tggagtgtat aggggacttg
      841 cacatcgatg accaccacac tgttgaggac tgtgggattg cgctgggcca agcgttcaag
      901 gaggcgcttg gctccgtgcg tggtatcaag aggttcgggc atgggtttgc accactggac
      961 gaggcgctga gccgcgctgt ggttgacttc tccaataggc ctttcgccgt ggtggagctg
     1021 ggcctgaagc gagaacgcat aggccagcta tccacagaga tgatcccgca cttcttggag
     1081 agtttcgcca ctgaggcgcg tatcaccatg catgtggact gtctgcgggg caccaacgac
     1141 caccaccgct ccgaatcagc tttcaaggcg ctcgccatcg ccatcagaga ggcaagaaca
     1201 cctacgggtc gcgatgacgt tccatccact aagggtgtcc tagcatagaa cacagcccac
     1261 agctaccacc atcaacaata tttatataga taacgtacac atagaaatca cacaaacaga
     1321 gtatttattc ttaactacat gaactaccat cagaccgtct gggccactat ataatgtgct
     1381 attcataaac gtgatcactc tacgtagcag gcgaccccag gtgaaaattt ttcagcgagc
     1441 tgccagattg tcaggtgaaa aactgaaaaa aacttctggg cgatgagctt gtggcgggaa
     1501 attaagtata taaagcagtt agtttcttct gcttcttgtg gttctgggta ttcttgtaat
     1561 atttggaata atagaaaacc aagactactg cctttctttt catattatag aggaagaaat
     1621 acgcacgaac acgatataga ggtaaatttt ttgcaaaaag ataccaaact ataaaagaaa
     1681 tagaagacta aactacaaaa aataaagaaa gtaatacata tatccaatat gactgagtta
     1741 gcagagcaag tttccatgcc tgaagggcaa caaagacgtg gtggttacgt tccacctcac
     1801 atccgtaacg gtggtggcag aagaggcgat tctgctcctc gtggtggttt caacaacggt
     1861 ggcggccgtg gtggtttctt cagaaacaac ggtgaccgtg gtggattcgg ctttgggtac
     1921 ctccgggtac gtgagttcgc cagcttcgag taggtagcct ttagtccatg cgttataggt
     1981 agcctttagt ccatgcgtta taggtagcct ttagtccatg cgttataggt agcctttagt
     2041 ccatgcgtta taggtagcct ttagtccatg cttaagagac actagagggt atataatgga
     2101 agctcgactt ccagcttggc aatccggtac tgttggtaaa gccaccgcgg ccgctaaaat
     2161 catgaacacc attccagcta gacacgtcgg cgacgttgcc gccagagatc ctttaccact
     2221 accccacatc tctagctccg tagcatctgg tatgaagcgt agcttcgcaa caatggcgat
     2281 gctgtataac gacaccggca acagcaatga cgttggagcc catgcgagaa gacctccaag
     2341 gaccctgagt aatagcaggt caaccagcgc gcatagagtc ccgctaggaa gctggtccgc
     2401 accgaactca cctccgaggc gtgccttgcc acatcatcca attaccgcgt ccttcgaccc
     2461 tgatgctagc attgtcattg cggggatcag gggggctggg aagagcacgc ttgcaattat
     2521 ggcaagcaca gcgatgaaaa gaaaaattgt agacttagaa tctgaattcc accatttgac
     2581 gggattgagt agttcaagct ataaaaaaac gcatggtcct gttgattatg gcagaaggca
     2641 aatcgccatc ttacaaaaca tcctgaattt gcataggact agagctattt tggtatgtag
     2701 ttggcttgag agagatgtac aggcaatgct gcaagacttt agtgtgagta atcctgtcat
     2761 ctatgttctg agagatgcga aggctatcga ggcacacctg aagggatatg ataagtctaa
     2821 ggtgggcacg cttttggacg ctacctcaac agttcttagg aggtgcaccc gttttgaatt
     2881 tttcaacgtt tcagaggaaa acctagatac ccactccgca agtactagtc ctccagctgt
     2941 ccccgatcag aggcacaccg ccccatatct taccctaaaa cgtgcggaaa gacactttct
     3001 gaagttttta agtcttatac tacccaaggg gacaatacct ttcgtagaaa gtgcgttccc
     3061 gctagcctct gtaccagttg aacagcgtcg tttcacctat gctctagctt tacctgtatc
     3121 agctctgctt gacaaagggg ttgatattca ggagttggat gttggagtcg atgccataga
     3181 gattatcgtg gacgatcttg caacttccga atctggacct acaagccccc tgggcttagc
     3241 tccgcacagg gcttcagaaa taagccgtgt cgtgggcgag attagaagag atactgttat
     3301 acctattatc cttcatgttg tgttccctga gagggcccta tatgaggaag cccttctagc
     3361 gttgtacatg acttatctta accatgcact taggcttgca cccgactacc tgaccgtaga
     3421 ccttgggctt gacagcggtt tgttaggaca gctaacaacc gtccaaggga ccacgaaagt
     3481 gataggaaat aaacaacttg cagaggtaaa ctccccgaga tggggtgatc cgagttggtt
     3541 acaagcatac gagaaagcac aaaacacagg gtgtgattta gtgaggttga ccagacccgc
     3601 tagcaatccc cgtgataaca cagatattag acagttccat gttgcagttg aggcggttgg
     3661 gggacctagg ctgccattta tcgcttataa caccgggaga ttgggaagaa cgtctatgtg
     3721 ctttaatgag atcctgaccc ccgtcacccc agtaccgacg aaggaggatg cgataggcct
     3781 tcgtaatccg gctcacagat acttacagcc tcctctaaca gcactggaag ccacacaagc
     3841 cctttattcc gcgtttgtgc acgatcctat gaaattatat gtgttcgggg ctaacgttgg
     3901 ctactccctt tcaccggcta tgcacaacgc agcgctaaaa gcttgcggaa taccacacca
     3961 ctataaaccg ctatcaactg ctaatatagg gactttgagg gaagtaatta gtgatccaca
     4021 atttgctgga gcttcagtag ggttaccgtt caaagttgag attatttcac ttacacatag
     4081 tctgtctaga cacgcaaagg ctatcggagc cgtgaatacg ttaatcccag tgcgtcatct
     4141 tactgcggac gggggcattc cagatgaagt cagtatgttt aataacattt ctcaggcggg
     4201 agcagtgaga gctctttatg gtgaaaatac tgattggatt ggaataagag catgtttaag
     4261 aagggggctt agcccagcaa acgcagtgag gtctacaagc accggtttag tgataggtgc
     4321 cggggggatg gcgagagccg cagtgtatgc aatgctgcaa cttggggtca aaaaaatttt
     4381 aattttcaac cgtactttcg ctaatgctga aaaactggtt cttcatttcg aaaacctgtt
     4441 agttcgtgat gcacttccat tgttaagcac ggggcccagg agtcatgata atacgtgctt
     4501 tcatatcatc cgttctagag atgacccatt gccagagaat ttcaaaaatc ctactatgat
     4561 cgtgagctgt attccaaccc atacagtcga taacacaccg gacccggaat ttacagtccc
     4621 cctacactgg cttgacaatc ctaccggcgg tatagttcta gagttagatt ataagtgctt
     4681 aacctcccca ctgctagagc agacacgtcg tgaagctcac cgtggctggg tggccatgga
     4741 cggacttgac ttactgcctg aacaaggctt tgcccagttc gaactgttta caggtagacg
     4801 tgcgccgcgt aggttgatga ggcgtgaagt gctacgtgcg tatccagacg accaggctaa
     4861 aagtcacacg gcacaactac agccccgtct aaacgggata gccacgcaaa tctctctcga
     4921 gtttcccccc gaagttgaag agcaagatga cgggacatta cctatgtctt gtgcccagga
     4981 atcagggatg gatagacacc cagcttcttg tccagaaagg gccgcatgtg cttcagccag
     5041 gataaacgta taatgatcga gagcttttga ttaagccttc tagtccaaaa aacacgtttt
     5101 tttgtcattt atttcatttt cttagaatag tttagtttat tcattttata gtcacgaatg
     5161 ttttatgatt ctatataggg ttgcaaacaa gcatttttca ttttatgtta aaacaatttc
     5221 aggtttacct tttattctgc ttgtggtgac gcgtgtatcc gcccgctctt ttggtcaccc
     5281 atgtatcgcc gggaaacagc tgcaagaatt actcgtgagt aaggaaagag tgaggaacta
     5341 tcgcatacct gcatttaaag atgccgattt gggcgcgaat cctttatttt ggcttcaccc
     5401 tcatactatt atcagggcca gaaaaaggaa gtgtttccct ccttcttgaa ttgatgttac
     5461 cctcataaag cacgtggcct cttatcgaga aagaaattac cgtcgctcgt gatttgtttg
     5521 caaaaagaac aaaactgaaa aaacccagac acgctcgact tcctgtcttc ctattgattg
     5581 cagcttccaa tttcgtcaca caacaaggtc ctagcgacgg ctcacaggtt ttgtaacaag
     5641 caatcgaagg ttctggaatg gcgggaaagg gtttagtacc acatgctatg atgcccactg
     5701 tgatctccag agcaaagttc gttcgatcgt actgttactc tctctctttc aaacagaatt
     5761 gtccgaatcg tgtgacaaca acagcctgtt ctcacacact cttttcttct aaccaagggg
     5821 gtggtttagt ttagtagaac ctcgtgaaac ttacatttac atatatataa acttgcataa
     5881 attggtcaat gcaagaaata catatttggt cttttctaat tcgtagtttt tcaagttctt
     5941 agatgctttc tttttctctt ttttacagat catcaaggaa gtaattatct actttttaca
     6001 acaaatataa aacaccgcgg tggcggccgc taaaatcatg ccacccaaga gaaagacgct
     6061 gaacgcggca gcggaagcta acgcccatgc agatggccac gcggacggta atgcggacgg
     6121 tcatgtagct aacaccgcgg cttcctctaa caacgcacgt ttcgctgact tgacgaatat
     6181 cgatacgcca gggttgggac cgactactac caccttattg gtggagcctg ccaggtctaa
     6241 aagacaaagg gtcagtcgtg cgtgtgacca atgcagagcc gctcgtgaaa aatgtgacgg
     6301 tattcagcct gcatgttttc cttgcgtgag tcagggcagg tcttgcacgt atcaggcttc
     6361 ccccaagaag cgtggcgttc agactggtta tattaggacc ttggaattgg cgttggcgtg
     6421 gatgtttgaa aacgtagcaa ggtccgaaga cgcgctacat aatctattag tcagggacgc
     6481 tggccaaggc tcagcgttac tggtagggaa ggattcaccg gcggcggaaa gactgcatgc
     6541 aaggtgggcg acctccaggg tcaataaaag tatcacaagg cttctccgtc aattagagct
     6601 gccgcccacg gctactgcca ctgcgtcaat catgcctcac gtcatggaac aacccttgag
     6661 tacgtcaata aaccctgtta atgatcgttt taatgggatt ccaaatccga cgccttacaa
     6721 ttctgacgct gctcttgacg ctataaccca aaccaacgat tacggttccg tgaataccca
     6781 cggaatcctg agcacctacc cccctccagc aacacacctt aacgaggcgt cagtagctct
     6841 agctccggga ggggcgcctc cgcgtccacc cccaccgtac gtggactcaa cgactaatca
     6901 ccctccctat catagcaacc tggtccctat ggcaaatttt ggttacagta cagttgatta
     6961 tgatgccatg gtagatgatt tagcgagtat tgaatatact gatgctgtag atgttgaccc
     7021 acaatttatg acgaatcttg ggttcgtccc tgggtgcaat ttcagtgaca ttaacacgta
     7081 tgaacaactc gagagtgctg gaggacagat gagaaagggt attgatctag ctactacact
     7141 cgaacgtatc gagaagaatt ttattatcac tgatcctagg cttcccgata atcccattat
     7201 ttttgcatct gatagtttct tggagctcac ggaatatagc cgtgaagaaa ttcttggaag
     7261 aaattgcagg tttctacaag gtccagagac tgatctaacc acagtgaaga agattcgaaa
     7321 tgctattgat aaccaaaccg aagtgacagt tcagctcatc aactacacca agagcggaaa
     7381 gaagttctgg aacattttcc acttgcaacc tatgcgtgat cagaagggag aagtacaata
     7441 ctttattgga gttcaactag acgggagcaa gcacgtagaa ccagttcgca atgtcattga
     7501 agaaaccgca gtgaaagagg gagaagacct ggtgaaaaaa acagctgtga atatcgatga
     7561 agcggttcga gaacttccta tgtcttgtgc acaagaatct attacttctt tgtacaagaa
     7621 ggctggttct gaaaacttgt acttccaata ataatgagta atgatcatct ctgcttttgt
     7681 gcgcgtatgt ttatgtatgt acctctctct ctatttctat ttttaaacca ccctctcaat
     7741 aaaataaaaa taataaagta tttttaagga aaagacgtgt ttaagcactg actttatcta
     7801 ctttttgtac gttttcattg atataatgtg ttttgtctct cccttttcta cgaaaatttc
     7861 aaaaattgac caaaaaaagg aatatatata cgaaaaacta ttatatttat atatcatagt
     7921 gttgataaaa aatgtttatc cattggaccg tgtatcatat ttatcgccgg cgaataaatt
     7981 aaggcgcgcc ttaatttatt cgccggcgat aagagcgacc tcatgctata cctgagaaag
     8041 caacctgacc tacaggaaag agttactcaa gaataagaat tttcgtttta aaacctaaga
     8101 gtcactttaa aatttgtata cacttatttt ttttataact tatttaataa taaaaatcat
     8161 aaatcataag aaattcgctg atcattactc gagtttgtat agttcatcca tgccatgtgt
     8221 aatcccagca gctgttacaa actcaagaag gaccatgtgg tctctctttt cgttgggatc
     8281 tttcgaaagg gcagattgtg tggacaggta atggttgtct ggtaaaagga cagggccatc
     8341 gccaattgga gtattttgtt gataatggtc tgctagttga acgcttccat cttcaatgtt
     8401 gtgtctaatt ttgaagttaa ctttgattcc attcttttgt ttgtctgcca tgatgtatac
     8461 attgtgtgag ttatagttgt attccaattt gtgtccaaga atgtttccat cttctttaaa
     8521 atcaatacct tttaactcga ttctattaac aagggtatca ccttcaaact tgacttcagc
     8581 acgtgtcttg tagttcccgt catctttgaa aaatatagtt ctttcctgta cataaccttc
     8641 gggcatggca ctcttgaaaa agtcatgccg tttcatatga tctgggtatc ttgaaaagca
     8701 ttgaacacca taagtgaaag tagtgacaag tgttggccat ggaacaggta gttttccagt
     8761 agtgcaaata aatttaaggg taagttttcc gtatgttgca tcaccttcac cctctccact
     8821 gacagaaaat ttgtgcccat taacatcacc atctaattca acaagaattg ggacaactcc
     8881 agtgaaaagt tcttctcctt tgtcgacgct agccatgatt ttagcggccg ctattaattt
     8941 agtgtgtgta tttgtgtttg tgtgtctata gaagtatagt aatttatgct gcaaaggtcc
     9001 taatgtataa ggaaagaata tttagagaaa agaagaaaac aagagtttta tatacataca
     9061 gagcacatgc atgccatatg atcatgtgtc gtcgcacaca tatatatatc tagaaaggat
     9121 aagcgattac cctaggataa gcgattaccc tagaggataa gcgattaccc ttgaggataa
     9181 ttgtttatcc taggataagc gattacccac tagtttaatt tattcttaag ctggcccggc
     9241 gataacccgg cgatacatgg gtgaccaaaa gagcgggcgg atacacgcgt caccacaagc
     9301 agaataaaag gtaaacctga aattgtttta acataaaatg aaaaatgctt gtttgcaacc
     9361 ctatatagaa tcataaaaca ttcgtgacta taaaatgaat aaactaaact attctaagaa
     9421 aatgaaataa atgacaaaaa aacgtgtttt ttggactaga aggcttaatc aaaagctctc
     9481 gatcattata cgtttatcct ggctgaagca catgcggccc tttctggaca agaagctggg
     9541 tgtctatcca tccctgattc ctgggcacaa gacataggta atgtcccgtc atcttgctct
     9601 tcaacttcgg ggggaaactc gagagagatt tgcgtggcta tcccgtttag acggggctgt
     9661 agttgtgccg tgtgactttt agcctggtcg tctggatacg cacgtagcac ttcacgcctc
     9721 atcaacctac gcggcgcacg tctacctgta aacagttcga actgggcaaa gccttgttca
     9781 ggcagtaagt caagtccgtc catggccacc cagccacggt gagcttcacg acgtgtctgc
     9841 tctagcagtg gggaggttaa gcacttataa tctaactcta gaactatacc gccggtagga
     9901 ttgtcaagcc agtgtagggg gactgtaaat tccgggtccg gtgtgttatc gactgtatgg
     9961 gttggaatac agctcacgat catagtagga tttttgaaat tctctggcaa tgggtcatct
    10021 ctagaacgga tgatatgaaa gcacgtatta tcatgactcc tgggccccgt gcttaacaat
    10081 ggaagtgcat cacgaactaa caggttttcg aaatgaagaa ccagtttttc agcattagcg
    10141 aaagtacggt tgaaaattaa aatttttttg accccaagtt gcagcattgc atacactgcg
    10201 gctctcgcca tccccccggc acctatcact aaaccggtgc ttgtagacct cactgcgttt
    10261 gctgggctaa gcccccttct taaacatgct cttattccaa tccaatcagt attttcacca
    10321 taaagagctc tcactgctcc cgcctgagaa atgttattaa acatactgac ttcatctgga
    10381 atgcccccgt ccgcagtaag atgacgcact gggattaacg tattcacggc tccgatagcc
    10441 tttgcgtgtc tagacagact atgtgtaagt gaaataatct caactttgaa cggtaaccct
    10501 actgaagctc cagcaaattg tggatcacta attacttccc tcaaagtccc tatattagca
    10561 gttgatagcg gtttatagtg gtgtggtatt ccgcaagctt ttagcgctgc gttgtgcata
    10621 gccggtgaaa gggagtagcc aacgttagcc ccgaacacat ataatttcat aggatcgtgc
    10681 acaaacgcgg aataaagggc ttgtgtggct tccagtgctg ttagaggagg ctgtaagtat
    10741 ctgtgagccg gattacgaag gcctatcgca tcctccttcg tcggtactgg ggtgacgggg
    10801 gtcaggatct cattaaagca catagacgtt cttcccaatc tcccggtgtt ataagcgata
    10861 aatggcagcc taggtccccc aaccgcctca actgcaacat ggaactgtct aatatctgtg
    10921 ttatcacggg gattgctagc gggtctggtc aacctcacta aatcacaccc tgtgttttgt
    10981 gctttctcgt atgcttgtaa ccaactcgga tcaccccatc tcggggagtt tacctctgca
    11041 agttgtttat ttcctatcac tttcgtggtc ccttggacgg ttgttagctg tcctaacaaa
    11101 ccgctgtcaa gcccaaggtc tacggtcagg tagtcgggtg caagcctaag tgcatggtta
    11161 agataagtca tgtacaacgc tagaagggct tcctcatata gggccctctc agggaacaca
    11221 acatgaagga taataggtat aacagtatct cttctaatct cgcccacgac acggcttatt
    11281 tctgaagccc tgtgcggagc taagcccagg gggcttgtag gtccagattc ggaagttgca
    11341 agatcgtcca cgataatctc tatggcatcg actccaacat ccaactcctg aatatcaacc
    11401 cctttgtcaa gcagagctga tacaggtaaa gctagagcat aggtgaaacg acgctgttca
    11461 actggtacag aggctagcgg gaacgcactt tctacgaaag gtattgtccc cttgggtagt
    11521 ataagactta aaaacttcag aaagtgtctt tccgcacgtt ttagggtaag atatggggcg
    11581 gtgtgcctct gatcggggac agctggagga ctagtacttg cggagtgggt atctaggttt
    11641 tcctctgaaa cgttgaaaaa ttcaaaacgg gtgcacctcc taagaactgt tgaggtagcg
    11701 tccaaaagcg tgcccacctt agacttatca tatcccttca ggtgtgcctc gatagccttc
    11761 gcatctctca gaacatagat gacaggatta ctcacactaa agtcttgcag cattgcctgt
    11821 acatctctct caagccaact acataccaaa atagctctag tcctatgcaa attcaggatg
    11881 ttttgtaaga tggcgatttg ccttctgcca taatcaacag gaccatgcgt ttttttatag
    11941 cttgaactac tcaatcccgt caaatggtgg aattcagatt ctaagtctac aatttttctt
    12001 ttcatcgctg tgcttgccat aattgcaagc gtgctcttcc cagcccccct gatccccgca
    12061 atgacaatgc tagcatcagg gtcgaaggac gcggtaattg gatgatgtgg caaggcacgc
    12121 ctcggaggtg agttcggtgc ggaccagctt cctagcggga ctctatgcgc gctggttgac
    12181 ctgctattac tcagggtcct tggaggtctt ctcgcatggg ctccaacgtc attgctgttg
    12241 ccggtgtcgt tatacagcat cgccattgtt gcgaagctac gcttcatacc agatgctacg
    12301 gagctagaga tgtggggtag tggtaaagga tctctggcgg caacgtcgcc gacgtgtcta
    12361 gctggaatgg tgttcatgat tttagcggcc gcggtggctt taccaacagt accggattgc
    12421 caagctggaa gtcgagcttc cattatatac cctctagtgt ctcttaagca tggactaaag
    12481 gctacctata acgcatggac taaaggctac ctataacgca tggactaaag gctacctata
    12541 acgcatggac taaaggctac ctataacgca tggactaaag gctacctact cgaagctggc
    12601 gaactcacgt acccggcgat aaggccgcaa attaaagcct tcgagcgtcc caaaaccttc
    12661 tcaagcaagg ttttcagtat aatgttacat gcgtacacgc gtctgtacag aaaaaaaaga
    12721 aaaatttgaa atataaataa cgttcttaat actaacataa ctataaaaaa ataaataggg
    12781 acctagactt caggttgtct aactccttcc ttttcggtta gagcggatgt ggggggaggg
    12841 cgtgaatgta agcgtgacat aactaattac atgatcatta ctcgaggtcg acttaaatac
    12901 ctgcttctac ggcaattcta actaaatctg cactcgtttt taagtttaat ttttccatta
    12961 ccaaacctct gtgcattttg acagtttttt ctgaaagacc cagtctggca gccacttctt
    13021 tatttcttag gcctgatgca accagagttg tgacttctaa ttgacgtggt gaaagagttt
    13081 ttaacatctc cgcagcacgt tctctacggg ccatgcccat gttcggttga tcgtcatcca
    13141 cttccacttg gctaccaagg aagtatagta attcatcatc gtcatcatag attggtgcca
    13201 ccaaaactgc attacgaaaa ggagtaccat cctttttgta attcaaaatc tcaaccaaca
    13261 cgggtttatg ctcacgaaca ccttgacgga tcttatctgt tagccagggt tcagtaccag
    13321 atcctgccaa aaatctacaa ttacgaccaa cacattcttc ctcagaatac ccagttagat
    13381 cggtaaaggc ctggttgata gcaatcaaag gattatcagc caatctggga tcactaacaa
    13441 cacttgctat gggtgaggcc tcgatcaaat ccaataccca ttgggctgga ggttggactt
    13501 caactctagt atcatctgct ccgaattcac ctccatattc gtcgatacca agtgcatcag
    13561 taaacatctg ttcaaattcg aaatctgcca tgtccaacgc gccatacgga gcagaatcat
    13621 gtggtgtaaa tccggggccg ggagaatccc catcacctaa catgtccaga tcaaaatcat
    13681 caagagcatc ggcatgtgcc atagctacat cctcaccgtc taaatgtaat tcgtcaccaa
    13741 gtgaaacgtc cgttggtggg gctacttttc tctttttttt cgggctagcc atgattttag
    13801 cggccgccaa aacttagatt agattgctat gctttctttc taatgagcaa gaagtaaaaa
    13861 aagttgtaat agaacaagaa aaatgaaact gaaacttgag aaattgaaga ccgtttatta
    13921 acttaaatat caatgggagg tcatcgaaag agaaaaaaat caaaaaaaaa attttcaaga
    13981 aaaagaaacg tgataaaaat ttttattgcc tttttcgacg aagaaaaaga aacgaggcgg
    14041 tctctttttt cttttccaaa cctttagtac gggtaattaa cgacacccta gaggaagaaa
    14101 gaggggaaat ttagtatgct gtgcttgggt gttttgaagt ggtacggcga tgcgcggagt
    14161 ccgagaaaat ctggaagagt aaaaaaggag tagaaacatt ttgaagctat cagctgtttc
    14221 ccgggagctg gaactcccac ctgcaacatg cgggatcctg agtaaataag cggccgccac
    14281 cgcggtggag ctcagatctt gacaccgatt atttaaagct gcagcatacg atatatatac
    14341 atgtgtatat atgtatacct atgaatgtca gtaagtatgt atacgaacag tatgatactg
    14401 aagatgacaa ggtaatgcat cattctatac gtgtcattct gaacgaggcg cgctttcctt
    14461 ttttcttttt gctttttctt tttttttctc ttgaactcca gaaaaaaaat ataaaagaga
    14521 tggaggaacg ggaaaaagtt agttgtggtg ataggtggca agtggtattc cgtaagaaca
    14581 acaagaaaag catttcatat tatggctgaa ctgagcgaac aagtgcaaaa tttaagcatc
    14641 aacgacaaca acgagaatgg ttatgttcct cctcacttaa gaggaaaacc aagaagtgcc
    14701 agaaataaca gtagcaacta caataacaac aacggcggct acaacggtgg ccgtggcggt
    14761 ggcagcttct ttagcaacaa ccgtcgtggt ggttacggca acggtggttt cttcggtgga
    14821 aacaacggtg gcagcggccg gccatttaaa tgtttaaacg gccacgctgg ccccctatag
    14881 tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
    14941 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
    15001 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg
    15061 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
    15121 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
    15181 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
    15241 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
    15301 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
    15361 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    15421 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    15481 aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc
    15541 tgtgcggtat ttcacaccgc atagggtaat aactgatata attaaattga agctctaatt
    15601 tgtgagttta gtattaggat ctctaccctt ggcgaaaagt cctctgccaa caatgatgat
    15661 atctgatcca ccacttacaa cttcgtcgag ggttctgtac tgctgaccca atatgcgtat
    15721 atataccaat ctaagtctgt gctccttcct tcgttcttcc ttctgttcgg agattaccga
    15781 atcaaaaaaa tttcaaggaa accgaaatca aaaaaaagaa taaaaaaaaa atgatgaatt
    15841 gaaaaggtgg tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
    15901 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
    15961 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
    16021 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
    16081 atgataataa tggtttctta gacgtcacgc gccgaggacg tcaggtggca cttttcgggg
    16141 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
    16201 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
    16261 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
    16321 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
    16381 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
    16441 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
    16501 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
    16561 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
    16621 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
    16681 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
    16741 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
    16801 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
    16861 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
    16921 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
    16981 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
    17041 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    17101 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    17161 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    17221 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    17281 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    17341 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    17401 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    17461 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    17521 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    17581 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    17641 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    17701 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    17761 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    17821 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    17881 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
    17941 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
    18001 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
    18061 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
    18121 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
    18181 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
    18241 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tcggaattaa
    18301 ccctcactaa agggaacaaa agctg
//
