LOCUS       sequence-340531-       14188 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of TAOK2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14188)
  AUTHORS   Ma X, Mandausch FJ, Wu Y, Sahoo VK, Ma W, Leoni G, Hostiuc M, 
            Wintgens JP, Qiu J, Kannaiyan N, Rossner MJ, Wehr MC
  TITLE     Comprehensive split TEV based protein-protein interaction screening 
            reveals TAOK2 as a key modulator of Hippo signalling to limit 
            growth.
  JOURNAL   Cell Signal. 2024 Jan;113:110917. doi: 
            10.1016/j.cellsig.2023.110917. Epub 2023 Oct 7.
  PUBMED    37813295
REFERENCE   2  (bases 1 to 14188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Signal."; date: "2024-01"; volume: "113:110917. doi"; pages: " 
            10.1016/j.cellsig.2023.110917. Epub 2023 Oct 7"
COMMENT     Sequence Label: pINDUCER21-puro_TAOK2
FEATURES             Location/Qualifiers
     source          1..14188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2397,2398..2416,2417..2439,
                     2440..2458,2459..2481,2482..2500)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6437..6461)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6514..6579
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6597..6623
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        6843..8021
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7074..8012
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7969..7989
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    8091..8124
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(8131..8748,8749..8754,8755..8790,8791..8793,8794..8829,
                     8830..8832,8833..8868,8869..8877)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    join(8930..9504,9505..9507,9508..9516)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9097..9114)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9324..9343
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9536..10135
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9536..9555)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10032..10052
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     10271..10290
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    10578..11166
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10631..10651)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11049..11060)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11241..11474
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(11593..11611)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11679..11783
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11784..11852,11853..12644)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12002..12021)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12815..13403
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13304..13323
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13557..13574
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13814..13835
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13850..13855,13856..13873,13874..13880)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13888..13904
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13893..13915
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13912..13928
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13912..13928
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13978..13997
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggctccacc atgccagctg ggggccgggc cgggagcctg
     2761 aaggacccag atgtggctga gctcttcttc aaggatgacc cagaaaagct cttctctgac
     2821 ctccgggaaa ttggccatgg cagctttgga gccgtatact ttgcccggga tgtccggaat
     2881 agtgaggtgg tggccatcaa gaagatgtcc tacagtggga agcagtccaa tgagaaatgg
     2941 caagacatca tcaaggaggt gcggttctta cagaagctcc ggcatcccaa caccattcag
     3001 taccggggct gttacctgag ggagcacacg gcttggctgg taatggagta ttgcctgggc
     3061 tcagcttctg accttctaga agtgcacaag aaaccccttc aggaggtaga gatcgcagct
     3121 gtgacccacg gggcgcttca gggcctggca tatctgcact cccacaacat gatccatagg
     3181 gatgtgaagg ctggaaacat cctgctgtca gagccagggt tagtgaagct aggggacttt
     3241 ggttctgcgt ccatcatggc acctgccaac tccttcgtgg gcaccccata ctggatggca
     3301 cccgaggtga tcctggccat ggatgagggg cagtacgatg gcaaagtgga cgtctggtcc
     3361 ttggggataa cctgcatcga gctggctgaa cggaaaccac cgctctttaa catgaatgcg
     3421 atgagtgcct tataccacat tgcacagaac gaatcccccg tgctccagtc aggacactgg
     3481 tctgagtact tccggaattt tgtcgactcc tgtcttcaga aaatccctca agacagacca
     3541 acctcagagg ttctcctgaa gcaccgcttt gtgctccggg agcggccacc cacagtcatc
     3601 atggacctga tccagaggac caaggatgcc gtgcgggagc tggacaacct gcagtaccgc
     3661 aagatgaaga agatcctgtt ccaagaggca cccaacggcc ctggtgccga ggccccagag
     3721 gaggaagagg aggccgagcc ctacatgcac cgggccggga ctctgaccag cctcgagagt
     3781 agccactcag tgcccagcat gtccatcagc gcctccagcc agagcagctc cgtcaacagc
     3841 ctagcagatg cctcagacaa cgaggaagag gaggaggagg aggaggaaga ggaggaggag
     3901 gaagaaggcc ctgaagcccg ggagatggcc atgatgcagg agggggagca cacagtcacc
     3961 tctcacagct ccattatcca ccggctgccg ggctctgaca acctatatga tgacccctac
     4021 cagccagaga taacccccag ccctctccag ccgcctgcag ccccagctcc cacttccacc
     4081 acctcttccg cccgccgccg ggcctactgc cgtaaccgag accactttgc caccatccga
     4141 accgcctccc tggtcagccg tcagatccag gagcatgagc aggactctgc gctgcgggag
     4201 cagctgagcg gctataagcg gatgcgacga cagcaccaga agcagctgct ggccctggag
     4261 tcacggctga ggggtgaacg ggaggagcac agtgcacggc tgcagcggga gcttgaggcg
     4321 cagcgggctg gctttggggc agaggcagaa aagctggccc ggcggcacca ggccataggt
     4381 gagaaggagg cacgagctgc ccaggccgag gagcggaagt tccagcagca catccttggg
     4441 cagcagaaga aggagctggc tgccctgctg gaggcacaga agcggaccta caaacttcgc
     4501 aaggaacagc tgaaggagga gctccaggag aaccccagca ctcccaagcg ggagaaggcc
     4561 gagtggctgc tgcggcagaa ggagcagctc cagcagtgcc aggcggagga ggaagcaggg
     4621 ctgctgcggc ggcagcgcca gtactttgag ctgcagtgtc gccagtacaa gcgcaagatg
     4681 ttgctggctc ggcacagcct ggaccaggac ctgctgcggg aggacctgaa caagaagcag
     4741 acccagaagg acttggagtg tgcactgctg cttcggcagc acgaggccac gcgggagctg
     4801 gagctgcggc agctccaggc cgtgcagcgc acgcgggctg agctcacccg cctgcagcac
     4861 cagacggagc tgggcaacca gctggagtac aacaagcggc gtgagcaaga gttgcggcag
     4921 aagcatgcgg cccaggttcg ccagcagccc aagagcctca aagtacgtgc aggccagcgc
     4981 cccccgggcc ttccactccc cattcctggg gctctgggcc cacccaacac aggcacccct
     5041 atagaacagc agccctgctc acctggccag gaggcagtcc tggaccaaag aatgcttggc
     5101 gaggaggagg aagcagttgg agagagaagg attctgggaa aggaaggggc cactttggag
     5161 cccaagcagc agaggattct gggggaagaa tcaggagccc ctagtcccag tccacaaaaa
     5221 catgggagcc tggttgatga ggaagtttgg ggtctgcctg aggagataga ggagcttagg
     5281 gtgccctccc ttgtacccca ggagaggagc attgttggcc aggaggaggc tgggacatgg
     5341 agcttgtggg ggaaggagga tgagagtctt ctggatgagg agtttgagct tggctgggtc
     5401 cagggcccag cactgactcc cgtccctgag gaggaggaag aagaggaaga gggggctccg
     5461 attgggaccc ctagggatcc tggagatggt tgtccttccc ccgacatccc tcctgaaccc
     5521 cctccaacac acctgaggcc ctgccctgcc agccagctcc ctggactcct gtcccatggc
     5581 ctcctggccg gcctctcctt tgcagtgggg tcctcctctg gcctcctgcc cctcctgctg
     5641 ctgctgctgc ttccattgct ggcagcccag ggtgggggtg gcctgcaggc agcgctgctg
     5701 gcccttgagg tggggctggt gggtctgggg gcctcctacc tgctcctttg tacagccctg
     5761 cacctgccct ccagtctttt cctactcctg gcccagggta ccgcactggg ggccgtcctg
     5821 ggcctgagct ggcgccgagg cctcatgggt gttcccctgg gccttggagc tgcctggctc
     5881 ttagcttggc caggcctagc tctacctctg gtggctatgg cagcgggggg cagatgggtg
     5941 cggcagcagg gcccccgggt gcgccggggc atatctcgac tctggttgcg ggttctgctg
     6001 cgcctgtcac ccatggcctt ccgggccctg cagggctgtg gggctgtggg ggaccggggt
     6061 ctgtttgcac tgtaccccaa aaccaacaag gatggcttcc gcagccgcct gcccgtccct
     6121 gggccccggc ggcgtaatcc ccgcaccacc caacacccat tagctctgtt ggcaagggtc
     6181 tgggtcctgt gcaagggctg gaactggcgt ctggcacggg ccagccaggg tttagcatcc
     6241 cacttgcccc cgtgggccat ccacacactg gccagctggg gcctgcttcg gggtgaacgg
     6301 cccacccgaa tcccccggct actaccacgc agccagcgcc agctagggcc ccctgcctcc
     6361 cgccagccac tgccagggac tctagccggg cggaggtcac gcacccgcca gtcccgggcc
     6421 ctgcccccct ggagggaccc agctttcttg tacaaagtgg ttgatatcca gcacagtggc
     6481 ggccgctcga gtctagaggg cccgcggttc gaagactaca aagaccatga cggtgattat
     6541 aaagatcatg acatcgatta caaggatgac gatgacaagt aggtaagtga ttcgaatacc
     6601 catacgacgt cccagactac gcttagtaat gattaattaa actagaaatt ctaccgggta
     6661 ggggaggcgc ttttcccaag gcagtctgga gtaacccacc caagatctgg cctccgcgcc
     6721 gggttttggc gcctcccgcg ggcgcccccc tcctcacggc gagccgcgta agatctggcc
     6781 tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc ctcacggcga gcctagtcgt
     6841 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     6901 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     6961 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     7021 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     7081 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     7141 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     7201 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     7261 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     7321 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     7381 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     7441 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     7501 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     7561 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     7621 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     7681 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     7741 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     7801 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     7861 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     7921 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     7981 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc catgctagta
     8041 aattgtccgc taaattctgg ccgtttttgg cttttttgtt agacggatcc ataacttcgt
     8101 ataatgtatg ctatacgaag ttatgccacc atgtctagac tggacaagag caaagtcata
     8161 aacggagctc tggaattact caatggtgtc ggtatcgaag gcctgacgac aaggaaactc
     8221 gctcaaaagc tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc
     8281 ctgctcgatg ccctgccaat cgagatgctg gacaggcatc atacccactt ctgccccctg
     8341 gaaggcgagt catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc
     8401 ctctcacatc gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac
     8461 gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca
     8521 ctgtacgctc tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag
     8581 catcaagtag caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg
     8641 agacaagcaa ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg
     8701 gaactaatca tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac
     8761 gcccttgacg attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt
     8821 gatatgctgc ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtaacta
     8881 agtaaggatc cgcggccgca ctagaggaat tccgcccctc tccccccccc ccctctccct
     8941 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     9001 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     9061 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     9121 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     9181 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     9241 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     9301 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     9361 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     9421 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     9481 tcctttgaaa aacacgatga taagcttgcc acaacccaca aggagacgac cttccatgac
     9541 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac
     9601 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca
     9661 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     9721 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     9781 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     9841 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     9901 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     9961 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
    10021 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
    10081 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgagcatg
    10141 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
    10201 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
    10261 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
    10321 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
    10381 cttcttgacg agttcttctg atgtacaagt aaagcggccg cgactctaga tcataatcag
    10441 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
    10501 cctgaaacat aaaatgaatg caattgttgt tgtttagtcc ctcccaattc gatatcaagc
    10561 ttatcgcgat agatcctaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
    10621 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
    10681 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
    10741 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
    10801 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
    10861 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
    10921 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
    10981 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
    11041 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
    11101 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
    11161 ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
    11221 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
    11281 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
    11341 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
    11401 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
    11461 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
    11521 gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta gatcattctt
    11581 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
    11641 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
    11701 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
    11761 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
    11821 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
    11881 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
    11941 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
    12001 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
    12061 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
    12121 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
    12181 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
    12241 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
    12301 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
    12361 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
    12421 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
    12481 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
    12541 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
    12601 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
    12661 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
    12721 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    12781 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    12841 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    12901 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    12961 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    13021 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    13081 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    13141 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    13201 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    13261 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    13321 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    13381 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    13441 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    13501 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    13561 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
    13621 ggccgattca ttaatgcagc aagctcatgg ctgactaatt ttttttattt atgcagaggc
    13681 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    13741 aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg
    13801 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    13861 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    13921 gctatgacat gattacgaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    13981 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
    14041 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
    14101 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
    14161 gttaaatatg tactacaaac ttagtagt
//
