LOCUS       sequence-340532-        6819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6819)
  TITLE     pCMV6-p85alpha-myc
REFERENCE   2  (bases 1 to 6819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMV6-p85alpha-Flag
FEATURES             Location/Qualifiers
     source          1..6819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..42
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     34..51
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     35..51
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        212..591
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        592..795
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     746..766
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3004..3027
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3253..3272)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(3700..3720)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3705..4034
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3885..4020
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4072..4091)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4073..4091)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4105..4121)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4105..4121)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4118..4140)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4129..4145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4153..4159,4160..4177,4178..4183))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4198..4219
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4336..4353)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4507..5095)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4587..4606)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5266..6057,6058..6126))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5889..5908
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6127..6231)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6258..6713
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6345..6364)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6555..6576
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
       61 atctatacat tgaatcaata ttggcaatta gccatattag tcattggtta tatagcataa
      121 atcaatattg gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat
      181 attggctcat gtccaatatg accgccatgt tgacattgat tattgactag ttattaatag
      241 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      301 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      361 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      421 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct
      481 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg
      541 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      601 ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      661 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      721 tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      781 tatataagca gagctcgttt agtgaaccgt cagaattaat tcagatccgc catgagtgca
      841 gagggctacc agtacagagc actgtacgac tacaagaagg agcgagagga agacattgac
      901 ctacacctgg gggacatact gactgtgaat aaaggctcct tagtggcact tggattcagt
      961 gatggccagg aagcccggcc tgaagatatt ggctggttaa atggctacaa tgaaaccact
     1021 ggggagaggg gagactttcc aggaacttac gttgaataca ttggaaggaa aagaatttca
     1081 ccccctactc ccaagcctcg gccccctcga ccgcttcctg ttgctccggg ttcttcaaaa
     1141 actgaagctg acacggagca gcaagcgttg ccccttcctg acctggccga gcagtttgcc
     1201 cctcctgatg ttgccccgcc tctccttata aagctcctgg aagccattga gaagaaagga
     1261 ctggaatgtt cgactctata cagaacacaa agctccagca accctgcaga attacgacag
     1321 cttcttgatt gtgatgccgc gtcagtggac ttggagatga tcgacgtaca cgtcttagca
     1381 gatgctttca aacgctatct cgccgactta ccaaatcctg tcattcctgt agctgtttac
     1441 aatgagatga tgtctttagc ccaagaacta cagagccctg aagactgcat ccagctgttg
     1501 aagaagctca ttagattgcc taatatacct catcagtgtt ggcttacgct tcagtatttg
     1561 ctcaagcatt ttttcaagct ctctcaagcc tccagcaaaa accttttgaa tgcaagagtc
     1621 ctctctgaga ttttcagccc cgtgcttttc agatttccag ccgccagctc tgataatact
     1681 gaacacctca taaaagcgat agagatttta atctcaacgg aatggaatga gagacagcca
     1741 gcaccagcac tgccccccaa accacccaag cccactactg tagccaacaa cagcatgaac
     1801 aacaatatgt ccttgcagga tgctgaatgg tactggggag acatctcaag ggaagaagtg
     1861 aatgaaaaac tccgagacac tgctgatggg acctttttgg tacgagacgc atctactaaa
     1921 atgcacggcg attacactct tacacctagg aaaggaggaa ataacaaatt aatcaaaatc
     1981 tttcaccgtg atggaaaata tggcttctct gatccattaa ccttcaactc tgtggttgag
     2041 ttaataaacc actaccggaa tgagtcttta gctcagtaca accccaagct ggatgtgaag
     2101 ttgctctacc cagtgtccaa ataccagcag gatcaagttg tcaaagaaga taatattgaa
     2161 gctgtaggga aaaaattaca tgaatataat actcaatttc aagaaaaaag tcgggaatat
     2221 gatagattat atgaggagta cacccgtact tcccaggaaa tccaaatgaa aagaacggct
     2281 atcgaagcat ttaatgaaac cataaaaata tttgaagaac aatgccaaac ccaggagcgg
     2341 tacagcaaag aatacataga gaagtttaaa cgcgaaggca acgagaaaga aattcaaagg
     2401 attatgcata accatgataa gctgaagtcg cgtatcagtg agatcattga cagtaggagg
     2461 aggttggaag aagacttgaa gaagcaggca gctgagtacc gagagatcga caaacgcatg
     2521 aacagtatta agccggacct catccagttg agaaagacaa gagaccaata cttgatgtgg
     2581 ctgacgcaga aaggtgtgcg gcagaagaag ctgaacgagt ggctggggaa tgaaaatacc
     2641 gaagatcaat actccctggt agaagatgat gaggatttgc cccaccatga cgagaagacg
     2701 tggaatgtcg ggagcagcaa ccgaaacaaa gcggagaacc tattgcgagg gaagcgagac
     2761 ggcactttcc ttgtccggga gagcagtaag cagggctgct atgcctgctc cgtagtggta
     2821 gacggcgaag tcaagcattg cgtcattaac aagactgcca ccggctatgg ctttgccgag
     2881 ccctacaacc tgtacagctc cctgaaggag ctggtgctac attatcaaca cacctccctc
     2941 gtgcagcaca atgactccct caatgtcaca ctagcatacc cagtatatgc acaacagagg
     3001 cgagactaca aggacgacga tgacaagtag ggatccccgg gcgagctcga attccgtggc
     3061 atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca
     3121 ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata
     3181 atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta
     3241 gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg
     3301 caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat
     3361 tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca
     3421 ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc
     3481 ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttaa
     3541 aataactata ccagcaggag gacgtccaga cacagcatag gctacctggc catgcccaac
     3601 cggtgggaca tttgagttgc ttgcttggca ctgtcctctc atgcgttggg tccactcagt
     3661 agatgcctgt tgaattgggt acgcggccag cttggctgtg gaatgtgtgt cagttagggt
     3721 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3781 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3841 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     3901 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3961 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     4021 taggcttttg caaaaagctc ctcgaggaac tgaaaaacca gaaagttaat tccctatagt
     4081 gagtcgtatt aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
     4141 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     4201 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     4261 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     4321 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     4381 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     4441 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     4501 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4561 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4621 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4681 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4741 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4801 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4861 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4921 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     4981 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5041 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     5101 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     5161 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     5221 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     5281 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     5341 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     5401 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     5461 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     5521 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     5581 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     5641 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     5701 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     5761 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     5821 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     5881 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     5941 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     6001 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     6061 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     6121 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     6181 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     6241 ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     6301 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     6361 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     6421 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     6481 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     6541 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     6601 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     6661 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttccattcg
     6721 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     6781 cagctggcga aagggggatg tgctgcaagg cgattaagt
//
