LOCUS       sequence-340534-       10039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPaint frame selector +2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10039)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 10039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCAS9-mCherry-Frame +2
FEATURES             Location/Qualifiers
     source          1..10039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             67..536
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             1005..1421
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1351..1373
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1389..1405
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        1432..1680
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1432..1452
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1603..1622
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        1701..1776
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        1817..2196
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2197..2400
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2351..2371
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2461..2470
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2470..2493
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2494..3198
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(2636..2654)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2904..2923
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3223..3276
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3277..7380
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3277..7380
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7393..7413
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     LTR             7592..8061
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8191..8208)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8362..8950)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8442..8461)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9110..9901,9902..9970))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9733..9752
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9971..10039,1..36))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
       61 tgcagcctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
      121 ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
      181 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt
      241 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
      301 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
      361 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg
      421 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
      481 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg
      541 ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc accgggaggc
      601 aagctggcca gcaacttatc tgtgtctgtc cgattgtcta gtgtctatga ctgattttat
      661 gcgcctgcgt cggtactagc tagttagcta actagctctg tatctggcgg acccgtggtg
      721 gaactgacga gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg
      781 gccgtttttg tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat
      841 atgtggttct ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc
      901 tttcggtttg gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt
      961 gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca
     1021 ctcccttaag tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt
     1081 cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta
     1141 acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca
     1201 aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg
     1261 aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc
     1321 ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc
     1381 gatcctccct ttatccagcc ctcactcctt ctctaggcgc cggccggatc cgagggccta
     1441 tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga
     1501 attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa
     1561 tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc
     1621 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc
     1681 gggccagtac ccaaaaagcg gttttagagc tagaaatagc aagttaaaat aaggctagtc
     1741 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttcgaattc cgatgtacgg
     1801 gccagatata cgcgttgaca ttgattattg tctagttatt aatagtaatc aattacgggg
     1861 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     1921 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     1981 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     2041 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     2101 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     2161 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     2221 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     2281 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     2341 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     2401 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaagct tccgcgtacg
     2461 gccaccatgg attataagga tgacgacgat aaggtgagca agggcgagga ggataacatg
     2521 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     2581 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     2641 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     2701 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     2761 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     2821 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     2881 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     2941 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     3001 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     3061 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
     3121 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     3181 atggacgagc tgtacaaggt cgctaccatc gagggatccg gagagggcag aggaagtctg
     3241 ctaacatgcg gtgacgtcga ggagaatcct ggcccaatgg acaagaagta cagcatcggc
     3301 ctggacatcg gcaccaacag cgtgggctgg gccgtgatca ccgacgagta caaggtgccc
     3361 agcaagaagt tcaaggtgct gggcaacacc gaccgccaca gcatcaagaa gaacctgatc
     3421 ggcgccctgc tgttcgacag cggcgagacc gccgaggcca cccgcctgaa gcgcaccgcc
     3481 cgccgccgct acacccgccg caagaaccgc atctgctacc tgcaggagat cttcagcaac
     3541 gagatggcca aggtggacga cagcttcttc caccgcctgg aggagagctt cctggtggag
     3601 gaggacaaga agcacgagcg ccaccccatc ttcggcaaca tcgtggacga ggtggcctac
     3661 cacgagaagt accccaccat ctaccacctg cgcaagaagc tggtggacag caccgacaag
     3721 gccgacctgc gcctgatcta cctggccctg gcccacatga tcaagttccg cggccacttc
     3781 ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg
     3841 gtgcagacct acaaccagct gttcgaggag aaccccatca acgccagcgg cgtggacgcc
     3901 aaggccatcc tgagcgcccg cctgagcaag agccgccgcc tggagaacct gatcgcccag
     3961 ctgcccggcg agaagaagaa cggcctgttc ggcaacctga tcgccctgag cctgggcctg
     4021 acccccaact tcaagagcaa cttcgacctg gccgaggacg ccaagctgca gctgagcaag
     4081 gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca gtacgccgac
     4141 ctgttcctgg ccgccaagaa cctgagcgac gccatcctgc tgagcgacat cctgcgcgtg
     4201 aacaccgaga tcaccaaggc ccccctgagc gccagcatga tcaagcgcta cgacgagcac
     4261 caccaggacc tgaccctgct gaaggccctg gtgcgccagc agctgcccga gaagtacaag
     4321 gagatcttct tcgaccagag caagaacggc tacgccggct acatcgacgg cggcgccagc
     4381 caggaggagt tctacaagtt catcaagccc atcctggaga agatggacgg caccgaggag
     4441 ctgctggtga agctgaaccg cgaggacctg ctgcgcaagc agcgcacctt cgacaacggc
     4501 agcatccccc accagatcca cctgggcgag ctgcacgcca tcctgcgccg ccaggaggac
     4561 ttctacccct tcctgaagga caaccgcgag aagatcgaga agatcctgac cttccgcatc
     4621 ccctactacg tcggccccct ggcccgcggc aacagccgct tcgcctggat gacccgcaag
     4681 agcgaggaga ccatcacccc ctggaacttc gaggaggtgg tggacaaggg cgccagcgcc
     4741 cagagcttca tcgagcgcat gaccaacttc gacaagaacc tgcccaacga gaaggtgctg
     4801 cccaagcaca gcctgctgta cgagtacttc accgtgtaca acgagctgac caaggtgaag
     4861 tacgtgaccg agggcatgcg caagcccgcc ttcctgagcg gcgagcagaa gaaggccatc
     4921 gtggacctgc tgttcaagac caaccgcaag gtgaccgtga agcagctgaa ggaggactac
     4981 ttcaagaaga tcgagtgctt cgacagcgtg gagatcagcg gcgtggagga ccgcttcaac
     5041 gccagcctgg gcacctacca cgacctgctg aagatcatca aggacaagga cttcctggac
     5101 aacgaggaga acgaggacat cctggaggac atcgtgctga ccctgaccct gttcgaggac
     5161 cgcgagatga tcgaggagcg cctgaagacc tacgcccacc tgttcgacga caaggtgatg
     5221 aagcagctga agcgccgccg ctacaccggc tggggccgcc tgagccgcaa gctgatcaac
     5281 ggcatccgcg acaagcagag cggcaagacc atcctggact tcctgaagag cgacggcttc
     5341 gccaaccgca acttcatgca gctgatccac gacgacagcc tgaccttcaa ggaggacatc
     5401 cagaaggccc aggtgagcgg ccagggcgac agcctgcacg agcacatcgc caacctggcc
     5461 ggcagccccg ccatcaagaa gggcatcctg cagaccgtga aggtggtgga cgagctggtg
     5521 aaggtgatgg gccgccacaa gcccgagaac atcgtgatcg agatggcccg cgagaaccag
     5581 accacccaga agggccagaa gaacagccgc gagcgcatga agcgcatcga ggagggcatc
     5641 aaggagctgg gcagccagat cctgaaggag caccccgtgg agaacaccca gctgcagaac
     5701 gagaagctgt acctgtacta cctgcagaac ggccgcgaca tgtacgtgga ccaggagctg
     5761 gacatcaacc gcctgagcga ctacgacgtg gaccacatcg tgccccagag cttcctgaag
     5821 gacgacagca tcgacaacaa ggtgctgacc cgcagcgaca agaaccgcgg caagagcgac
     5881 aacgtgccca gcgaggaggt ggtgaagaag atgaagaact actggcgcca gctgctgaac
     5941 gccaagctga tcacccagcg caagttcgac aacctgacca aggccgagcg cggcggcctg
     6001 agcgagctgg acaaggccgg cttcatcaag cgccagctgg tggagacccg ccagatcacc
     6061 aagcacgtgg cccagatcct ggacagccgc atgaacacca agtacgacga gaacgacaag
     6121 ctgatccgcg aggtgaaggt gatcaccctg aagagcaagc tggtgagcga cttccgcaag
     6181 gacttccagt tctacaaggt gcgcgagatc aacaactacc accacgccca cgacgcctac
     6241 ctgaacgccg tggtgggcac cgccctgatc aagaagtacc ccaagctgga gagcgagttc
     6301 gtgtacggcg actacaaggt gtacgacgtg cgcaagatga tcgccaagag cgagcaggag
     6361 atcggcaagg ccaccgccaa gtacttcttc tacagcaaca tcatgaactt cttcaagacc
     6421 gagatcaccc tggccaacgg cgagatccgc aagcgccccc tgatcgagac caacggcgag
     6481 accggcgaga tcgtgtggga caagggccgc gacttcgcca ccgtgcgcaa ggtgctgagc
     6541 atgccccagg tgaacatcgt gaagaagacc gaggtgcaga ccggcggctt cagcaaggag
     6601 agcatcctgc ccaagcgcaa cagcgacaag ctgatcgccc gcaagaagga ctgggacccc
     6661 aagaagtacg gcggcttcga cagccccacc gtggcctaca gcgtgctggt ggtggccaag
     6721 gtggagaagg gcaagagcaa gaagctgaag agcgtgaagg agctgctggg catcaccatc
     6781 atggagcgca gcagcttcga gaagaacccc atcgacttcc tggaggccaa gggctacaag
     6841 gaggtgaaga aggacctgat catcaagctg cccaagtaca gcctgttcga gctggagaac
     6901 ggccgcaagc gcatgctggc cagcgccggc gagctgcaga agggcaacga gctggccctg
     6961 cccagcaagt acgtgaactt cctgtacctg gccagccact acgagaagct gaagggcagc
     7021 cccgaggaca acgagcagaa gcagctgttc gtggagcagc acaagcacta cctggacgag
     7081 atcatcgagc agatcagcga gttcagcaag cgcgtgatcc tggccgacgc caacctggac
     7141 aaggtgctga gcgcctacaa caagcaccgc gacaagccca tccgcgagca ggccgagaac
     7201 atcatccacc tgttcaccct gaccaacctg ggcgcccccg ccgccttcaa gtacttcgac
     7261 accaccatcg accgcaagcg ctacaccagc accaaggagg tgctggacgc caccctgatc
     7321 caccagagca tcaccggcct gtacgagacc cgcatcgacc tgagccagct gggcggcgac
     7381 agccgcgccg accccaagaa gaagcgcaag gtgtgagcgg ccgcatcgat aaaataaaag
     7441 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag
     7501 ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag
     7561 ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
     7621 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca
     7681 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc
     7741 cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc
     7801 aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc
     7861 tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc
     7921 gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca
     7981 gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta
     8041 cccgtcagcg ggggtctttc acatgcagca tgtatcaaaa ttaatttggt tttttttctt
     8101 aagtatttac attaaatggc catagttgca ttaatgaatc ggccaacgcg cggggagagg
     8161 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8221 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8281 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8341 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8401 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8461 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8521 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8581 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8641 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8701 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8761 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     8821 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8881 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8941 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     9001 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9061 gcggccgcaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9121 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9181 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9241 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9301 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9361 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9421 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9481 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9541 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9601 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9661 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9721 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9781 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9841 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9901 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9961 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10021 tgagcggata catatttga
//
