LOCUS       sequence-340541-       11956 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the genetically-encoded fluorescent norepinephrine(NE) 
            sensor GRAB_NE1m in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11956)
  AUTHORS   Feng J, Zhang C, Lischinsky JE, Jing M, Zhou J, Wang H, Zhang Y, 
            Dong A, Wu Z, Wu H, Chen W, Zhang P, Zou J, Hires SA, Zhu JJ, Cui G,
            Lin D, Du J, Li Y
  TITLE     A Genetically Encoded Fluorescent Sensor for Rapid and Specific In 
            Vivo Detection of Norepinephrine.
  JOURNAL   Neuron. 2019 May 22;102(4):745-761.e8. doi: 
            10.1016/j.neuron.2019.02.037. Epub 2019 Mar 25.
  PUBMED    30922875
REFERENCE   2  (bases 1 to 11956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2019.02.037"; journalName: "Neuron"; date: 
            "2019-05-22- 22"; volume: "102"; issue: "4"; pages: "745-761.e8"
COMMENT     Sequence Label: pSin-NE1m
FEATURES             Location/Qualifiers
     source          1..11956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        677..695
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     677..694
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     sig_peptide     8353..8412
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     protein_bind    8416..8440
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     9543..9564
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(9670..9691)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9931..9950)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        10821..10925
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10926..10994,10995..11786)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11144..11163)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagctcg tatggacata ttgtcgttag aacgcggcta caattaatac ataaccttat
      661 gtatcataca catacgattt aggtgacact atagattgac ggcgtagtac acactattga
      721 atcaaacagc cgaccaattg cactaccatc acaatggaga agccagtagt aaacgtagac
      781 gtagaccccc agagtccgtt tgtcgtgcaa ctgcaaaaaa gcttcccgca atttgaggta
      841 gtagcacagc aggtcactcc aaatgaccat gctaatgcca gagcattttc gcatctggcc
      901 agtaaactaa tcgagctgga ggttcctacc acagcgacga tcttggacat aggcagcgca
      961 ccggctcgta gaatgttttc cgagcaccag tatcattgtg tctgccccat gcgtagtcca
     1021 gaagacccgg accgcatgat gaaatatgcc agtaaactgg cggaaaaagc gtgcaagatt
     1081 acaaacaaga acttgcatga gaagattaag gatctccgga ccgtacttga tacgccggat
     1141 gctgaaacac catcgctctg ctttcacaac gatgttacct gcaacatgcg tgccgaatat
     1201 tccgtcatgc aggacgtgta tatcaacgct cccggaacta tctatcatca ggctatgaaa
     1261 ggcgtgcgga ccctgtactg gattggcttc gacaccaccc agttcatgtt ctcggctatg
     1321 gcaggttcgt accctgcgta caacaccaac tgggccgacg agaaagtcct tgaagcgcgt
     1381 aacatcggac tttgcagcac aaagctgagt gaaggtagga caggaaaatt gtcgataatg
     1441 aggaagaagg agttgaagcc cgggtcgcgg gtttatttct ccgtaggatc gacactttat
     1501 ccagaacaca gagccagctt gcagagctgg catcttccat cggtgttcca cttgaatgga
     1561 aagcagtcgt acacttgccg ctgtgataca gtggtgagtt gcgaaggcta cgtagtgaag
     1621 aaaatcacca tcagtcccgg gatcacggga gaaaccgtgg gatacgcggt tacacacaat
     1681 agcgagggct tcttgctatg caaagttact gacacagtaa aaggagaacg ggtatcgttc
     1741 cctgtgtgca cgtacatccc ggccaccata tgcgatcaga tgactggtat aatggccacg
     1801 gatatatcac ctgacgatgc acaaaaactt ctggttgggc tcaaccagcg aattgtcatt
     1861 aacggtagga ctaacaggaa caccaacacc atgcaaaatt accttctgcc gatcatagca
     1921 caagggttca gcaaatgggc taaggagcgc aaggatgatc ttgataacga gaaaatgctg
     1981 ggtactagag aacgcaagct tacgtatggc tgcttgtggg cgtttcgcac taagaaagta
     2041 cattcgtttt atcgcccacc tggaacgcag acctgcgtaa aagtcccagc ctcttttagc
     2101 gcttttccca tgtcgtccgt atggacgacc tctttgccca tgtcgctgag gcagaaattg
     2161 aaactggcat tgcaaccaaa gaaggaggaa aaactgctgc aggtctcgga ggaattagtc
     2221 atggaggcca aggctgcttt tgaggatgct caggaggaag ccagagcgga gaagctccga
     2281 gaagcacttc caccattagt ggcagacaaa ggcatcgagg cagccgcaga agttgtctgc
     2341 gaagtggagg ggctccaggc ggacatcgga gcagcattag ttgaaacccc gcgcggtcac
     2401 gtaaggataa tacctcaagc aaatgaccgt atgatcggac agtatatcgt tgtctcgcca
     2461 aactctgtgc tgaagaatgc caaactcgca ccagcgcacc cgctagcaga tcaggttaag
     2521 atcataacac actccggaag atcaggaagg tacgcggtcg aaccatacga cgctaaagta
     2581 ctgatgccag caggaggtgc cgtaccatgg ccagaattcc tagcactgag tgagagcgcc
     2641 acgttagtgt acaacgaaag agagtttgtg aaccgcaaac tataccacat tgccatgcat
     2701 ggccccgcca agaatacaga agaggagcag tacaaggtta caaaggcaga gcttgcagaa
     2761 acagagtacg tgtttgacgt ggacaagaag cgttgcgtta agaaggaaga agcctcaggt
     2821 ctggtcctct cgggagaact gaccaaccct ccctatcatg agctagctct ggagggactg
     2881 aagacccgac ctgcggtccc gtacaaggtc gaaacaatag gagtgatagg cacaccgggg
     2941 tcgggcaagt cagctattat caagtcaact gtcacggcac gagatcttgt taccagcgga
     3001 aagaaagaaa attgtcgcga aattgaggcc gacgtgctaa gactgagggg tatgcagatt
     3061 acgtcgaaga cagtagattc ggttatgctc aacggatgcc acaaagccgt agaagtgctg
     3121 tacgttgacg aagcgttcgc gtgccacgca ggagcactac ttgccttgat tgctatcgtc
     3181 aggccccgca agaaggtagt actatgcgga gaccccatgc aatgcggatt cttcaacatg
     3241 atgcaactaa aggtacattt caatcaccct gaaaaagaca tatgcaccaa gacattctac
     3301 aagtatatct cccggcgttg cacacagcca gttacagcta ttgtatcgac actgcattac
     3361 gatggaaaga tgaaaaccac gaacccgtgc aagaagaaca ttgaaatcga tattacaggg
     3421 gccacaaagc cgaagccagg ggatatcatc ctgacatgtt tccgcgggtg ggttaagcaa
     3481 ttgcaaatcg actatcccgg acatgaagta atgacagccg cggcctcaca agggctaacc
     3541 agaaaaggag tgtatgccgt ccggcaaaaa gtcaatgaaa acccactgta cgcgatcaca
     3601 tcagagcatg tgaacgtgtt gctcacccgc actgaggaca ggctagtgtg gaaaaccttg
     3661 cagggcgacc catggattaa gcagctcact aacataccta aaggaaactt tcaggctact
     3721 atagaggact gggaagctga acacaaggga ataattgctg caataaacag ccccactccc
     3781 cgtgccaatc cgttcagctg caagaccaac gtttgctggg cgaaagcatt ggaaccgata
     3841 ctagccacgg ccggtatcgt acttaccggt tgccagtgga gcgaactgtt cccacagttt
     3901 gcggatgaca aaccacattc ggccatttac gccttagacg taatttgcat taagtttttc
     3961 ggcatggact tgacaagcgg actgttttct aaacagagca tcccactaac gtaccatccc
     4021 gccgattcag cgaggccggt agctcattgg gacaacagcc caggaacccg caagtatggg
     4081 tacgatcacg ccattgccgc cgaactctcc cgtagatttc cggtgttcca gctagctggg
     4141 aagggcacac aacttgattt gcagacgggg agaaccagag ttatctctgc acagcataac
     4201 ctggtcccgg tgaaccgcaa tcttcctcac gccttagtcc ccgagtacaa ggagaagcaa
     4261 cccggcccgg tcgaaaaatt cttgaaccag ttcaaacacc actcagtact tgtggtatca
     4321 gaggaaaaaa ttgaagctcc ccgtaagaga atcgaatgga tcgccccgat tggcatagcc
     4381 ggtgcagata agaactacaa cctggctttc gggtttccgc cgcaggcacg gtacgacctg
     4441 gtgttcatca acattggaac taaatacaga aaccaccact ttcagcagtg cgaagaccat
     4501 gcggcgacct taaaaaccct ttcgcgttcg gccctgaatt gccttaaccc aggaggcacc
     4561 ctcgtggtga agtcctatgg ctacgccgac cgcaacagtg aggacgtagt caccgctctt
     4621 gccagaaagt ttgtcagggt gtctgcagcg agaccagatt gtgtctcaag caatacagaa
     4681 atgtacctga ttttccgaca actagacaac agccgtacac ggcaattcac cccgcaccat
     4741 ctgaattgcg tgatttcgtc cgtgtatgag ggtacaagag atggagttgg agccgcgccg
     4801 tcataccgca ccaaaaggga gaatattgct gactgtcaag aggaagcagt tgtcaacgca
     4861 gccaatccgc tgggtagacc aggcgaagga gtctgccgtg ccatctataa acgttggccg
     4921 accagtttta ccgattcagc cacggagaca ggcaccgcaa gaatgactgt gtgcctagga
     4981 aagaaagtga tccacgcggt cggccctgat ttccggaagc acccagaagc agaagccttg
     5041 aaattgctac aaaacgccta ccatgcagtg gcagacttag taaatgaaca taacatcaag
     5101 tctgtcgcca ttccactgct atctacaggc atttacgcag ccggaaaaga ccgccttgaa
     5161 gtatcactta actgcttgac aaccgcgcta gacagaactg acgcggacgt aaccatctat
     5221 tgcctggata agaagtggaa ggaaagaatc gacgcggcac tccaacttaa ggagtctgta
     5281 acagagctga aggatgaaga tatggagatc gacgatgagt tagtatggat tcatccagac
     5341 agttgcttga agggaagaaa gggattcagt actacaaaag gaaaattgta ttcgtacttc
     5401 gaaggcacca aattccatca agcagcaaaa gacatggcgg agataaaggt cctgttccct
     5461 aatgaccagg aaagtaatga acaactgtgt gcctacatat tgggtgagac catggaagca
     5521 atccgcgaaa agtgcccggt cgaccataac ccgtcgtcta gcccgcccaa aacgttgccg
     5581 tgcctttgca tgtatgccat gacgccagaa agggtccaca gacttagaag caataacgtc
     5641 aaagaagtta cagtatgctc ctccaccccc cttcctaagc acaaaattaa gaatgttcag
     5701 aaggttcagt gcacgaaagt agtcctgttt aatccgcaca ctcccgcatt cgttcccgcc
     5761 cgtaagtaca tagaagtgcc agaacagcct accgctcctc ctgcacaggc cgaggaggcc
     5821 cccgaagttg tagcgacacc gtcaccatct acagctgata acacctcgct tgatgtcaca
     5881 gacatctcac tggatatgga tgacagtagc gaaggctcac ttttttcgag ctttagcgga
     5941 tcggacaact ctattactag tatggacagt tggtcgtcag gacctagttc actagagata
     6001 gtagaccgaa ggcaggtggt ggtggctgac gttcatgccg tccaagagcc tgcccctatt
     6061 ccaccgccaa ggctaaagaa gatggcccgc ctggcagcgg caagaaaaga gcccactcca
     6121 ccggcaagca atagctctga gtccctccac ctctcttttg gtggggtatc catgtccctc
     6181 ggatcaattt tcgacggaga gacggcccgc caggcagcgg tacaacccct ggcaacaggc
     6241 cccacggatg tgcctatgtc tttcggatcg ttttccgacg gagagattga tgagctgagc
     6301 cgcagagtaa ctgagtccga acccgtcctg tttggatcat ttgaaccggg cgaagtgaac
     6361 tcaattatat cgtcccgatc agccgtatct tttcctctac gcaagcagag acgtagacgc
     6421 aggagcagga ggactgaata ctgactaacc ggggtaggtg ggtacatatt ttcgacggac
     6481 acaggccctg ggcacttgca aaagaagtcc gttctgcaga accagcttac agaaccgacc
     6541 ttggagcgca atgtcctgga aagaattcat gccccggtgc tcgacacgtc gaaagaggaa
     6601 caactcaaac tcaggtacca gatgatgccc accgaagcca acaaaagtag gtaccagtct
     6661 cgtaaagtag aaaatcagaa agccataacc actgagcgac tactgtcagg actacgactg
     6721 tataactctg ccacagatca gccagaatgc tataagatca cctatccgaa accattgtac
     6781 tccagtagcg taccggcgaa ctactccgat ccacagttcg ctgtagctgt ctgtaacaac
     6841 tatctgcatg agaactatcc gacagtagca tcttatcaga ttactgacga gtacgatgct
     6901 tacttggata tggtagacgg gacagtcgcc tgcctggata ctgcaacctt ctgccccgct
     6961 aagcttagaa gttacccgaa aaaacatgag tatagagccc cgaatatccg cagtgcggtt
     7021 ccatcagcga tgcagaacac gctacaaaat gtgctcattg ccgcaactaa aagaaattgc
     7081 aacgtcacgc agatgcgtga actgccaaca ctggactcag cgacattcaa tgtcgaatgc
     7141 tttcgaaaat atgcatgtaa tgacgagtat tgggaggagt tcgctcggaa gccaattagg
     7201 attaccactg agtttgtcac cgcatatgta gctagactga aaggccctaa ggccgccgca
     7261 ctatttgcaa agacgtataa tttggtccca ttgcaagaag tgcctatgga tagattcgtc
     7321 atggacatga aaagagacgt gaaagttaca ccaggcacga aacacacaga agaaagaccg
     7381 aaagtacaag tgatacaagc cgcagaaccc ctggcgactg cttacttatg cgggattcac
     7441 cgggaattag tgcgtaggct tacggccgtc ttgcttccaa acattcacac gctttttgac
     7501 atgtcggcgg aggattttga tgcaatcata gcagaacact tcaagcaagg cgacccggta
     7561 ctggagacgg atatcgcatc attcgacaaa agccaagacg acgctatggc gttaaccggt
     7621 ctgatgatct tggaggacct gggtgtggat caaccactac tcgacttgat cgagtgcgcc
     7681 tttggagaaa tatcatccac ccatctacct acgggtactc gttttaaatt cggggcgatg
     7741 atgaaatccg gaatgttcct cacacttttt gtcaacacag ttttgaatgt cgttatcgcc
     7801 agcagagtac tagaagagcg gcttaaaacg tccagatgtg cagcgttcat tggcgacgac
     7861 aacatcatac atggagtagt atctgacaaa gaaatggctg agaggtgcgc cacctggctc
     7921 aacatggagg ttaagatcat cgacgcagtc atcggtgaga gaccacctta cttctgcggc
     7981 ggatttatct tgcaagattc ggttacttcc acagcgtgcc gcgtggcgga tcccctgaaa
     8041 aggctgttta agttgggtaa accgctccca gccgacgacg agcaagacga agacagaaga
     8101 cgcgctctgc tagatgaaac aaaggcgtgg tttagagtag gtataacagg cactttagca
     8161 gtggccgtga cgacccggta tgaggtagac aatattacac ctgtcctact ggcattgaga
     8221 acttttgccc agagcaaaag agcattccaa gccatcagag gggaaataaa gcatctctac
     8281 ggtggtccta aatagtcagc atagtacatt tcatctgact aataccacaa caccaccacc
     8341 tctagatcca ccatggagac agacacactc ctgctatggg tactgctgct ctgggttcca
     8401 ggttccactg gtgacacaag tttgtacaaa aaagttggca ccaccggtgg ctccctgcag
     8461 ccggacgcgg gcaacgcgag ctggaacggg accgaggcgc cggggggcgg cgcccgggcc
     8521 accccttact ccctgcaggt gacgctgacg ctggtgtgcc tggccggcct gctcatgctg
     8581 ctcaccgtgt tcggcaacgt gctcgtcatc atcgccgtgt tcacgagccg cgcgctcaag
     8641 gcgccccaaa acctcttcct ggtgtctctg gcctcggccg acatcctggt ggccacgctc
     8701 gtcatccctt tctcgctggc caacgaggtc atgggctact ggtacttcgg caaggcttgg
     8761 tgcgagatct acctggcgct cgacgtgctc ttctgcacgt cgtccatcgt gcacctgtgc
     8821 gccatcagcc tggaccgcta ctggtccatc acacaggcca tcgagtacaa cctgaagcgc
     8881 acgccgcgcc gcatcaaggc catcatcatc accgtgtggg tcatctcggc cgtcatctcc
     8941 ttcccgccgc tcatctccat cgagaagaag ggcggcggcg gcggcccgca gccggccgag
     9001 ccgcgctgcg agatcaacga ccagaagtgg tacgtcatct cgtcgtgcat cggctccttc
     9061 ttcgctccct gcctcatcat gatcctggtc tacgtgcgca tctaccagat cgccaagcgt
     9121 cgcacccgcg tgccacccag ccgccggggt ccggacgccg tcgccgcgcc gccggggggc
     9181 accgagcgca ggcccaacgg tctgggcccc gagcgcagcg cgggcccggg gggcgcagag
     9241 gccgaaccgc tgcccaccca gctcaacggc gcccctggcg agcccgcgcc ggccgggccg
     9301 cgcgacaccg acgcgctgga cctggaggag ggaggaaacg tctatatcaa ggccgacaag
     9361 cagaagaacg gcatcaaggc gaacttccac atccgccaca acatcgagga cggcggcgtg
     9421 cagctcgcct accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     9481 gacaaccact acctgagcgt gcagtccaaa ctttcgaaag accccaacga gaagcgcgat
     9541 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     9601 tacaagggcg gtaccggagg gagcatggtg agaaagggcg aggagctgtt caccggggtg
     9661 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     9721 gagggtgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     9781 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     9841 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     9901 tacatccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     9961 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
    10021 gaggacggca acatcctggg gcacaagctg gagtacaaca ccggagcagc agcacgctgg
    10081 cgcgggcggc agaaccgcga gaagcgcttc acgttcgtgc tggccgtggt catcggagtg
    10141 ttcgtggtgt gctggttccc cttcttcttc acctacacgc tcacggccgt cgggtgctcc
    10201 gtgccacgca cgctcttcaa attcttcttc tggttcggct actgcaacag ctcgttgaac
    10261 ccggtcatct acaccatctt caaccacgat ttccgccgcg ccttcaagaa gatcctctgt
    10321 cggggggaca ggaagcggat cgtgttgtaa gcatgcaggc cttgggccca atgatccgac
    10381 cagcaaaact cgatgtactt ccgaggaact gatgtgcata atgcatcagg ctggtacatt
    10441 agatccccgc ttaccgcggg caatatagca acactaaaaa ctcgatgtac ttccgaggaa
    10501 gcgcagtgca taatgctgcg cagtgttgcc acataaccac tatattaacc atttatctag
    10561 cggacgccaa aaactcaatg tatttctgag gaagcgtggt gcataatgcc acgcagcgtc
    10621 tgcataactt ttattatttc ttttattaat caacaaaatt ttgtttttaa catttcaaaa
    10681 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaggggaatt cctcgataat taagcggccg
    10741 ctcgagcggc cgcttaatta atcgagggga attaattctt gaagacgaaa gggccaggtg
    10801 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
    10861 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
    10921 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
    10981 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
    11041 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
    11101 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
    11161 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
    11221 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    11281 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    11341 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    11401 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
    11461 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    11521 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    11581 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    11641 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    11701 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    11761 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    11821 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    11881 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    11941 agatcaaagg atcttc
//
