LOCUS       sequence-340557-       10007 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Tiam1(DH-PH) fused to 
            mNeonGreen-eDHFR(69K6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10007)
  AUTHORS   Suzuki S, Nakamura A, Hatano Y, Yoshikawa M, Yoshii T, Sawada S, 
            Atsuta-Tsunoda K, Aoki K, Tsukiji S
  TITLE     A chemogenetic platform for controlling plasma membrane signaling 
            and synthetic signal oscillation.
  JOURNAL   Cell Chem Biol. 2022 Sep 15;29(9):1446-1464.e10. doi: 
            10.1016/j.chembiol.2022.06.005. Epub 2022 Jul 13.
  PUBMED    35835118
REFERENCE   2  (bases 1 to 10007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2022.06.005"; journalName: "Cell Chem Biol"; 
            date: "2022-09-15- 15"; volume: "29"; issue: "9"; pages: 
            "1446-1464.e10"
COMMENT     Sequence Label: pPBpuro-mNG-eDHFR(69K6)-Tiam1
FEATURES             Location/Qualifiers
     source          1..10007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(90..545)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(227..248)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     439..458
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     672..694
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     686..703
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     687..703
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     713..732
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        713..731
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   761..795
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1098..1477
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1750..2766
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2690..2712
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2774..2793
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2839..2848
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2845..3552
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      3100..3109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6000..6549
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6144..6161)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6371..6390
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6552..7151
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6552..6571)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7048..7068
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7178..7195)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7184..7408
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7455..7471)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7479..7495
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7503..7509,7510..7527,7528..7533))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7548..7569
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   7803..7865
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7906..7926)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7906..7924)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7945..7961)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7969..7985
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7993..7999,8000..8017,8018..8023))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8038..8059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8176..8193)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8347..8935)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8427..8446)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9106..9897,9898..9966))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9729..9748
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9967..10007,1..64))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
       61 cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt
      121 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
      181 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
      241 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
      301 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
      361 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
      421 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
      481 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
      541 cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
      601 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
      661 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga
      721 ctcactatag ggcgaattgg ggcgcgccat tctagattaa ccctagaaag atagtctgcg
      781 taaaattgac gcatgcattc ttgaaatatt gctctctctt tctaaatagc gcgaatccgt
      841 cgctgtgcat ttaggacatc tcagtcgccg cttggagctc ccgtgaggcg tgcttgtcaa
      901 tgcggtaagt gtcactgatt ttgaactata acgaccgcgt gagtcaaaat gacgcatgat
      961 tatcttttac gtgactttta agatttaact catacgataa ttatattgtt atttcatgtt
     1021 ctacttacgt gataacttat tatatatata ttttcttgtt atagatatca actagaatgc
     1081 tagcatgggc ccatctcgac attgattatt gactagttat taatagtaat caattacggg
     1141 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     1201 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1261 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1321 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1381 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1441 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
     1501 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
     1561 attattttgt gcagcgatgg gggcgggggg gggggggggg ggggcggggc ggggcggggc
     1621 gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc
     1681 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
     1741 cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
     1801 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     1861 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
     1921 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
     1981 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc
     2041 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc
     2101 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg
     2161 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc
     2221 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
     2281 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
     2341 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg
     2401 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc
     2461 gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc
     2521 cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg
     2581 ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg
     2641 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
     2701 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
     2761 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg
     2821 ggatcctcac gtcaattggc caccatggtg agcaagggcg aggaggataa catggcctct
     2881 ctcccagcga cacatgagtt acacatcttt ggctccatca acggtgtgga ctttgacatg
     2941 gtgggtcagg gcaccggcaa tccaaatgat ggttatgagg agttaaacct gaagtccacc
     3001 aagggtgacc tccagttctc cccctggatt ctggtccctc atatcgggta tggcttccat
     3061 cagtacctgc cctaccctga cgggatgtcg cctttccagg ccgccatggt agatggctcc
     3121 ggctaccaag tccatcgcac aatgcagttt gaagatggtg cctcccttac tgttaactac
     3181 cgctacacct acgagggaag ccacatcaaa ggagaggccc aggtgaaggg gactggtttc
     3241 cctgctgacg gtcctgtgat gaccaactcg ctgaccgctg cggactggtg caggtcgaag
     3301 aagacttacc ccaacgacaa aaccatcatc agtaccttta agtggagtta caccactgga
     3361 aatggcaagc gctaccggag cactgcgcgg accacctaca cctttgccaa gccaatggcg
     3421 gctaactatc tgaagaacca gccgatgtac gtgttccgta agacggagct caagcactcc
     3481 aagaccgagc tcaacttcaa ggagtggcaa aaggccttta ccgatgtgat gggcatggac
     3541 gagctctaca agtctgggtc cgcagccagt agcgccggaa gtatgatcag tctgattgcg
     3601 gcgttagcgg tagatcgcgt tatcggcatg gaaaacgcca tgccgtggaa cctgcctgcc
     3661 gatctcgcct ggtttaaacg caacacctta aataaacccg tgattatggg ccgccatacc
     3721 tgggaatcaa tcggtcgtcc gttgccagga cgcaaaaata ttatcctcag cagtcaaccg
     3781 ggtacggaca aaaaaaagaa aaagaaagat cgcgtaacgt gggtgaagtc ggtggatgaa
     3841 gccatcgcgg cgtgtggtga cgtaccagaa atcatggtga ttggcggcgg tcgcgtttat
     3901 gaacagttct tgccaaaagc gcaaaaactg tatctgacgc atatcgacgc agaagtggaa
     3961 ggcgacaccc atttcccgga ttacgagccg gatgactggg aatcggtatt cagcgaattc
     4021 cacgatgctg atgcgcagaa ctctcacagc tattgctttg agattctgga gcggcggctg
     4081 tacaagtccg gactcagatc tcgacaaggt agtggtgctg gctctggtgc tggtagtggc
     4141 gctggttccg gtgctggctc tggcgcgcct cgagcaatga acccctctga ccagagccca
     4201 tctcctcagg actccacggg gcctcagctg gcgaccatga gacaactctc ggatgcagat
     4261 aagctgcgca aggtgatctg cgagctcctg gagacggagc gcacctacgt gaaggattta
     4321 aactgtctta tggagagata cctaaagcct cttcaaaaag aaacttttct cacccaggat
     4381 gagcttgacg tgctttttgg aaatttaacg gaaatggtag agtttcaagt agaattcctt
     4441 aaaactctag aagatggagt gagactggta cctgatttgg aaaagcttga gaaggttgat
     4501 caatttaaga aagtgctgtt ctctctgggg ggatcattcc tgtattatgc tgaccgcttc
     4561 aagctctaca gtgccttctg cgccagccac acaaaagttc ccaaggtcct ggtgaaagcc
     4621 aagacagaca cggctttcaa ggcattcttg gatgcccaga acccgaagca gcagcactca
     4681 tccacgctgg agtcgtacct catcaagccc atccagagga tcctcaagta cccacttctg
     4741 ctcagggagc tgttcgccct gaccgatgcg gagagcgagg agcactacca cctggacgtg
     4801 gccatcaaga ccatgaacaa ggttgccagt cacatcaatg agatgcagaa aatccatgaa
     4861 gagtttgggg ctgtgtttga ccagctgatt gctgaacaga ctggtgggaa aaaagaggtt
     4921 gcagatctga gcatgggaga cctgcttttg cacactaccg tgatctggct gaacccgccg
     4981 gcctcgctgg gcaagtggaa aaaggaacca gagttggcag cattcgtctt caaaactgct
     5041 gtggtccttg tgtataaaga tggttccaaa cagaagaaga aacttgtagg atctcacagg
     5101 ctttccattt atgaggactg ggaccccttc agatttcgac acatgatccc cacggaagcg
     5161 ctgcaggttc gagctttggc gagtgcagat gcagaggcaa atgccgtgtg tgaaattgtc
     5221 catgtaaaat ccgagtctga agggaggccg gagagggtct ttcacttgtg ctgcagctcc
     5281 ccagagagcc gaaaggattt cctaaaggct gtgcattcaa tcctgcgtga taagcacaga
     5341 agacagctcc tcaaaaccga gagccttccc tcatcccagc aatatgtccc ttttggaggc
     5401 aaaagattgt gtgcactgaa gggggccagg ccggccatga gcagggcagt gtctgcccca
     5461 agcaagtctc ttgggaggag gaggcggcgg ctggctcgaa acaggtttac cattgattct
     5521 gatgccgtct ccgcaagcag cccggagaaa gagtcccagc agccccccgg tggcggggac
     5581 actgaccgat gggtagagga gcagtttgat cttgctcagt atgaggagca agatgacatc
     5641 aaggagacag acatcctcag tgacgatgat gagttctgtg agtccgtgaa gggtgcctca
     5701 gtggacagag acctgcagga gcggcttcag gccacctcca tcagtcagcg ggaaagaggc
     5761 cggaaaaccc tggatagtca cgcgtcccgc atggcacagc tcaagaagca agctgccctg
     5821 tcggggatca atggaggcct ggagagcgca agcgaggaag tcatttgggt taggcgtgaa
     5881 gactttgccc cctccaggaa actgaacact gagatctgac cgcgggcccg ggataagtca
     5941 actaacttaa gctagcaacg gtttccctct agcgggatca attccgcccc ccccccctaa
     6001 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     6061 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     6121 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     6181 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     6241 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     6301 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     6361 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     6421 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     6481 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     6541 acgataatac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     6601 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     6661 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     6721 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     6781 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     6841 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     6901 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     6961 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     7021 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     7081 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     7141 ccggtgcctg atcgatgatc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc
     7201 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     7261 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     7321 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     7381 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaacctg cagcccaagc
     7441 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     7501 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     7561 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7621 cggatcttaa aagttttgtt actttataga agaaattttg agtttttgtt tttttttaat
     7681 aaataaataa acataaataa attgtttgtt gaatttatta ttagtatgta agtgtaaata
     7741 taataaaact taatatctat tcaaattaat aaataaacct cgatatacag accgataaaa
     7801 cacatgcgtc aattttacgc atgattatct ttaacgtacg tcacaatatg attatctttc
     7861 tagggttaat ctagtatacg cgtttaatta atccagcttt tgttcccttt agtgagggtt
     7921 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7981 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     8041 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8101 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8161 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8221 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8281 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8341 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8401 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8461 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8521 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8581 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8641 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8701 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8761 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8821 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8881 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8941 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9001 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9061 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9121 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9181 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9241 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9301 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9361 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9421 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9481 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9541 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9601 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9661 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9721 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9781 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9841 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9901 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9961 actcatactc ttcctttttc aatattattg aagcatttat cagggtt
//
