LOCUS       sequence-340558-       10109 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cRaf fused to mNeonGreen-eDHFR(69K6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10109)
  AUTHORS   Suzuki S, Nakamura A, Hatano Y, Yoshikawa M, Yoshii T, Sawada S, 
            Atsuta-Tsunoda K, Aoki K, Tsukiji S
  TITLE     A chemogenetic platform for controlling plasma membrane signaling 
            and synthetic signal oscillation.
  JOURNAL   Cell Chem Biol. 2022 Sep 15;29(9):1446-1464.e10. doi: 
            10.1016/j.chembiol.2022.06.005. Epub 2022 Jul 13.
  PUBMED    35835118
REFERENCE   2  (bases 1 to 10109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2022.06.005"; journalName: "Cell Chem Biol"; 
            date: "2022-09-15- 15"; volume: "29"; issue: "9"; pages: 
            "1446-1464.e10"
COMMENT     Sequence Label: pPBpuro-cRaf-mNG-eDHFR(69K6)
FEATURES             Location/Qualifiers
     source          1..10109
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1668..2684
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2608..2630
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2692..2711
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      4700..4709
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4706..5413
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      4961..4970
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6015..6564
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6159..6176)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6386..6405
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6567..7166
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6567..6586)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7063..7083
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7193..7210)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7199..7423
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7470..7486)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7494..7510
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7518..7524,7525..7542,7543..7548))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7563..7584
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   7818..7880
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7921..7941)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7921..7939)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7960..7976)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7984..8000
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8008..8014,8015..8032,8033..8038))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8053..8074
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8191..8208)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8362..8950)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8442..8461)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9121..9912,9913..9981))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9744..9763
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9982..10086)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg gsgcgcgccr gggggggggg gggggggggg gggggggggg ggggsgrggs
     1561 ggrgrggkgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
     1621 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc
     1681 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
     1741 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
     1801 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
     1861 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
     1921 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
     1981 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     2041 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     2101 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     2161 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     2221 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     2281 ccggggrggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     2341 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     2401 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     2461 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     2521 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     2581 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     2641 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     2701 tggttattgt gctgtctcat cattttggca aagaattggg atcatggagc acatacaggg
     2761 agcttggaag acgatcagca atggttttgg attcaaagat gccgtgtttg atggctccag
     2821 ctgcatctct cctacaatag ttcagcagtt tggctatcag cgccgggcat cagatgatgg
     2881 caaactcaca gatccttcta agacaagcaa cactatccgt gttttcttgc cgaacaagca
     2941 aagaacagtg gtcaatgtgc gaaatggaat gagcttgcat gactgcctta tgaaagcact
     3001 caaggtgagg ggcctgcaac cagagtgctg tgcagtgttc agacttctcc acgaacacaa
     3061 aggtaaaaaa gcacgcttag attggaatac tgatgctgcg tctttgattg gagaagaact
     3121 tcaagtagat ttcctggatc atgttcccct cacaacacac aactttgctc ggaagacgtt
     3181 cctgaagctt gccttctgtg acatctgtca gaaattcctg ctcaatggat ttcgatgtca
     3241 gacttgtggc tacaaatttc atgagcactg tagcaccaaa gtacctacta tgtgtgtgga
     3301 ctggagtaac atcagacaac tcttattgtt tccaaattcc actattggtg atagtggagt
     3361 cccagcacta ccttctttga ctatgcgtcg tatgcgagag tctgtttcca ggatgcctgt
     3421 tagttctcag cacagatatt ctacacctca cgccttcacc tttaacacct ccagtccctc
     3481 atctgaaggt tccctctccc agaggcagag gtcgacatcc acacctaatg tccacatggt
     3541 cagcaccacc ctgcctgtgg acagcaggat gattgaggat gcaattcgaa gtcacagcga
     3601 atcagcctca ccttcagccc tgtccagtag ccccaacaat ctgagcccaa caggctggtc
     3661 acagccgaaa acccccgtgc cagcacaaag agagcgggca ccagtatctg ggacccagga
     3721 gaaaaacaaa attaggcctc gtggacagag agattcaagc tattattggg aaatagaagc
     3781 cagtgaagtg atgctgtcca ctcggattgg gtcaggctct tttggaactg tttataaggg
     3841 taaatggcac ggagatgttg cagtaaagat cctaaaggtt gtcgacccaa ccccagagca
     3901 attccaggcc ttcaggaatg aggtggctgt tctgcgcaaa acacggcatg tgaacattct
     3961 gcttttcatg gggtacatga caaaggacaa cctggcaatt gtgacccagt ggtgcgaggg
     4021 cagcagcctc tacaaacacc tgcatgtcca ggagaccaag tttcagatgt tccagctaat
     4081 tgacattgcc cggcagacgg ctcagggaat ggactatttg catgcaaaga acatcatcca
     4141 tagagacatg aaatccaaca atatatttct ccatgaaggc ttaacagtga aaattggaga
     4201 ttttggtttg gcaacagtaa agtcacgctg gagtggttct cagcaggttg aacaacctac
     4261 tggctctgtc ctctggatgg ccccagaggt gatccgaatg caggataaca acccattcag
     4321 tttccagtcg gatgtctact cctatggcat cgtattgtat gaactgatga cgggggagct
     4381 tccttattct cacatcaaca accgagatca gatcatcttc atggtgggcc gaggatatgc
     4441 ctccccagat cttagtaagc tatataagaa ctgccccaaa gcaatgaaga ggctggtagc
     4501 tgactgtgtg aagaaagtaa aggaagagag gcctcttttt ccccagatcc tgtcttccat
     4561 tgagctgctc caacactctc taccgaagat caaccggagc gcttccgagc catccttgca
     4621 tcgggcagcc cacactgagg atatcaatgc ttgcacgctg accacgtccc cgaggctgcc
     4681 tgtcttcgat ccaccggtcg ccaccatggt gagcaagggc gaggaggata acatggcctc
     4741 tctcccagcg acacatgagt tacacatctt tggctccatc aacggtgtgg actttgacat
     4801 ggtgggtcag ggcaccggca atccaaatga tggttatgag gagttaaacc tgaagtccac
     4861 caagggtgac ctccagttct ccccctggat tctggtccct catatcgggt atggcttcca
     4921 tcagtacctg ccctaccctg acgggatgtc gcctttccag gccgccatgg tagatggctc
     4981 cggctaccaa gtccatcgca caatgcagtt tgaagatggt gcctccctta ctgttaacta
     5041 ccgctacacc tacgagggaa gccacatcaa aggagaggcc caggtgaagg ggactggttt
     5101 ccctgctgac ggtcctgtga tgaccaactc gctgaccgct gcggactggt gcaggtcgaa
     5161 gaagacttac cccaacgaca aaaccatcat cagtaccttt aagtggagtt acaccactgg
     5221 aaatggcaag cgctaccgga gcactgcgcg gaccacctac acctttgcca agccaatggc
     5281 ggctaactat ctgaagaacc agccgatgta cgtgttccgt aagacggagc tcaagcactc
     5341 caagaccgag ctcaacttca aggagtggca aaaggccttt accgatgtga tgggcatgga
     5401 cgagctgtac aagtctgggt ccgcagccag tagcgccgga agtatgatca gtctgattgc
     5461 ggcgttagcg gtagatcgcg ttatcggcat ggaaaacgcc atgccgtgga acctgcctgc
     5521 cgatctcgcc tggtttaaac gcaacacctt aaataaaccc gtgattatgg gccgccatac
     5581 ctgggaatca atcggtcgtc cgttgccagg acgcaaaaat attatcctca gcagtcaacc
     5641 gggtacggac aaaaaaaaga aaaagaaaga tcgcgtaacg tgggtgaagt cggtggatga
     5701 agccatcgcg gcgtgtggtg acgtaccaga aatcatggtg attggcggcg gtcgcgttta
     5761 tgaacagttc ttgccaaaag cgcaaaaact gtatctgacg catatcgacg cagaagtgga
     5821 aggcgacacc catttcccgg attacgagcc ggatgactgg gaatcggtat tcagcgaatt
     5881 ccacgatgct gatgcgcaga actctcacag ctattgcttt gagattctgg agcggcggta
     5941 acccgggata agtcaactaa cttaagctag caacggtttc cctctagcgg gatcaattcc
     6001 gccccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     6061 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     6121 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     6181 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     6241 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     6301 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     6361 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     6421 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     6481 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     6541 tttcctttga aaaacacgat aataccatga ccgagtacaa gcccacggtg cgcctcgcca
     6601 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     6661 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     6721 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     6781 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     6841 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     6901 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     6961 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     7021 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     7081 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     7141 gcatgacccg caagcccggt gcctgatcga tgatctagag ctcgctgatc agcctcgact
     7201 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     7261 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     7321 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     7381 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     7441 acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7501 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7561 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7621 aacctgtcgt gccagcggat cttaaaagtt ttgttacttt atagaagaaa ttttgagttt
     7681 ttgttttttt ttaataaata aataaacata aataaattgt ttgttgaatt tattattagt
     7741 atgtaagtgt aaatataata aaacttaata tctattcaaa ttaataaata aacctcgata
     7801 tacagaccga taaaacacat gcgtcaattt tacgcatgat tatctttaac gtacgtcaca
     7861 atatgattat ctttctaggg ttaatctagt atacgcgttt aattaatcca gcttttgttc
     7921 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     7981 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     8041 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     8101 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8161 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8221 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8281 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8341 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8401 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8461 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8521 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8581 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8641 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8701 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8761 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     8821 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8881 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8941 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     9001 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9061 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9121 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9181 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9241 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9301 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9361 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9421 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9481 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9541 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9601 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9661 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9721 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9781 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9841 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9901 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9961 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    10021 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    10081 tccgcgcaca tttccccgaa aagtgccac
//
