LOCUS       sequence-340578-        9436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for SARS-CoV-2 (B.1.351) spike used in the Spike 
            Display system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9436)
  AUTHORS   Javanmardi K, Chou CW, Terrace CI, Annapareddy A, Kaoud TS, Guo Q, 
            Lutgens J, Zorkic H, Horton AP, Gardner EC, Nguyen G, Boutz DR, 
            Goike J, Voss WN, Kuo HC, Dalby KN, Gollihar JD, Finkelstein IJ
  TITLE     Rapid characterization of spike variants via mammalian cell surface 
            display.
  JOURNAL   Mol Cell. 2021 Dec 16;81(24):5099-5111.e8. doi: 
            10.1016/j.molcel.2021.11.024.
  PUBMED    34919820
REFERENCE   2  (bases 1 to 9436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.11"; journalName: "Mol Cell"; date: 
            "2021-12-16- 16"; volume: "81"; issue: "24"; pages: "5099-5111.e8"
COMMENT     Sequence Label: Spike Display_B.1.351
FEATURES             Location/Qualifiers
     source          1..9436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1..225
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(358..377)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    509..556
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             564..1586
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             564..1586
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    1716..1837
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1753..1772)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1807..1826
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1886..1902)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1886..1902)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1899..1921)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1910..1926
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1934..1940,1941..1958,1959..1964))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1979..2000
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2117..2134)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2288..2876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2368..2387)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3047..3838,3839..3907))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTHLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3670..3689
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3908..4011)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4087..4106)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4278..4657
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4658..4861
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4812..4832
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4863..4881
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4884..4902
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4912..4936
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          5055..5187
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     5170..5189
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        5232..5250
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     5296..5355
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             9070..9135
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             9142..9165
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             9172..9195
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             9238..9384
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(join(9431..9436,1..12))
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
       61 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
      121 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
      181 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
      241 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
      301 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
      361 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
      421 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
      481 ttgattaggg tgatggttca cgtacctaga agttcctatt ccgaagttcc tattctctag
      541 aaagtatagg aacttccttg gccaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
      601 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
      661 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
      721 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
      781 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
      841 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
      901 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
      961 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     1021 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     1081 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     1141 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     1201 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     1261 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     1321 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     1381 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     1441 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     1501 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     1561 gcactcgtcc gagggcaaag gaatagcacg tactacgaga tttcgattcc accgccgcct
     1621 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     1681 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     1741 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     1801 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     1861 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     1921 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     1981 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     2041 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     2101 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     2161 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     2221 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2281 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2341 ggaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     2401 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     2461 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     2521 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     2581 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     2641 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     2701 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     2761 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2821 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     2881 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2941 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     3001 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     3061 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3121 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     3181 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     3241 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     3301 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     3361 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     3421 aacgatcaag gtgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     3481 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     3541 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     3601 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     3661 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     3721 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3781 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3841 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3901 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3961 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cacacatttc
     4021 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
     4081 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     4141 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     4201 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
     4261 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
     4321 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     4381 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     4441 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     4501 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     4561 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     4621 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     4681 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     4741 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     4801 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     4861 tctccctatc agtgatagag atctccctat cagtgataga gatcgtcgac gagctcgttt
     4921 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
     4981 ccgggaccga tccagcctcc ggactctagc gtttaaactt aagctgcaga agttggtcgt
     5041 gaggcactgg gcaggtaagt atcaaggtta caagacaggt ttaaggagac caatagaaac
     5101 tgggcttgtc gagacagaga agactcttgc gtttctgata ggcacctatt ggtcttactg
     5161 acatccactt tgcctttctc tccacaggtg tccactccca gttcaattac agctcttaag
     5221 gctagagtac ttaatacgac tcactatagg ctagcctcga gaattcacgc gtggtacctc
     5281 tagagtcgac ccgggatgga gacagacaca ctcctgctat gggtactgct gctctgggtt
     5341 ccaggttcca ctggtgacat gttcgtgttc ctggtgctcc tgcctctggt gagcagccag
     5401 tgcgtgaact tcaccacccg aacccagctc ccaccagcct acaccaacag ctttacacgg
     5461 ggcgtgtact accctgacaa ggtgttcaga tctagcgtcc tgcacagcac tcaggacctc
     5521 ttcctgccgt tcttcagcaa cgtgacatgg ttccacgcca tccacgtgag cggcacaaac
     5581 ggaaccaagc ggtttgccaa ccccgtcctg ccattcaatg atggagttta cttcgccagt
     5641 accgagaaga gtaacatcat ccggggctgg atcttcggca ccaccctgga tagcaaaaca
     5701 cagagcctcc tgatcgtgaa caatgccacg aacgtcgtga tcaaggtgtg cgagttccag
     5761 ttttgcaatg atcctttcct gggtgtgtac taccacaaga acaacaagag ctggatggaa
     5821 agcgagttca gagtctacag cagcgccaac aactgcacat tcgagtacgt ctctcagcct
     5881 tttctgatgg accttgaggg gaaacaaggc aacttcaaga acctgagaga attcgtgttc
     5941 aagaacatcg acggctactt caaaatctac tccaagcaca cacccatcaa cctggtccgg
     6001 ggcctccctc agggcttcag cgccctggaa cccctggtcg acctgcccat aggcatcaac
     6061 ataacgcggt tccaaaccct gcatatctcc tacctgactc ctggcgacag cagcagcgga
     6121 tggaccgccg gagctgcagc ctactatgtg ggctacctgc aacctagaac cttcctgctg
     6181 aagtacaacg agaacggcac aatcacagac gccgtcgact gcgccctgga ccctctctct
     6241 gagacaaagt gcaccctgaa gtccttcacc gtggaaaagg gcatctacca gaccagcaac
     6301 ttccgggtgc agcctacaga gagcatcgtg cgatttccaa acattaccaa cctctgcccc
     6361 ttcggcgagg tgtttaacgc cacaagattt gcctccgttt acgcctggaa tagaaagaga
     6421 atcagcaatt gtgtggccga ctactccgtg ctgtataaca gcgcctcttt cagcaccttc
     6481 aagtgctacg gcgtttcccc aacaaagctg aatgacctgt gcttcaccaa cgtgtacgcc
     6541 gactccttcg taattagagg cgatgaggtg cggcagatcg caccaggcca gaccggtaac
     6601 atcgctgact acaactataa gctgcctgat gattttacag gctgcgtgat cgcctggaac
     6661 tctaacaacc tggatagcaa ggtgggcggc aactacaact acctgtaccg gctgtttcgc
     6721 aagtctaacc tgaaaccttt cgagagagac atctccacag agatctacca ggccggttct
     6781 acaccttgta acggggtgaa gggcttcaac tgttacttcc ctctgcaaag ctacggcttc
     6841 cagcctacct acggagtcgg ctaccagcca taccgggtgg tcgtgctgtc cttcgagtta
     6901 ctccacgccc ccgccaccgt ctgcggtcct aagaagtcca ccaatctggt taagaacaaa
     6961 tgcgtgaact tcaacttcaa cggcctgacc gggaccggcg tgctgaccga aagcaacaaa
     7021 aagttcctcc ccttccagca gttcggccgt gatatcgctg acaccacaga tgccgtcaga
     7081 gatccacaga ccctggaaat cctggatatt acaccctgct ccttcggagg agtttctgtg
     7141 atcacccccg ggaccaatac cagcaaccag gtggctgtgc tgtaccaagg tgttaactgc
     7201 accgaggttc ctgtggccat ccacgccgat cagctgacac ctacttggag agtgtactcc
     7261 actggctcca atgtgttcca gaccagggcc ggatgtctga tcggcgccga gcacgtgaat
     7321 aacagttacg agtgcgacat ccctatcggc gccggcatct gtgccagcta ccagacccag
     7381 acaaacagcc ctgggtctgc ttcctctgta gctagccaga gcatcatcgc ctacaccatg
     7441 agcctgggcg tggagaacag cgtggcctat tccaacaact ctatcgccat tcccaccaac
     7501 tttacaatta gcgtcacaac agagatcctg cccgtgagca tgaccaagac cagcgtggac
     7561 tgtacaatgt acatctgtgg cgacagcact gaatgcagca acctgctgct gcaatacggc
     7621 tccttttgca cccaactgaa ccgggcgctg accggaatcg ccgtggaaca ggacaaaaat
     7681 acccaggagg tgttcgccca agtgaagcag atctacaaga ccccacctat caaggacttc
     7741 ggcggcttta actttagcca gattctccct gatccttcta agcctagcaa gcggagccct
     7801 atcgaggatc tgctgttcaa caaggtcacc ctggccgatg ccggctttat caaacagtat
     7861 ggcgattgcc tgggcgacat agccgccaga gatctgatct gcgcccagaa attcaacggc
     7921 ctgacagttc tcccacctct gctgaccgac gagatgatcg ctcagtacac ctctgccctg
     7981 ctggctggca ccatcacatc tgggtggaca tttggcgccg gccccgccct gcagatcccc
     8041 tttcccatgc agatggccta tagattcaac ggaatcggcg tgacccagaa cgtgctgtat
     8101 gaaaaccaga agctgatcgc taaccagttc aattctgcca tcggcaagat ccaggactcc
     8161 ctctcctcta cccccagcgc cctgggcaaa ctgcaggacg tggtgaatca gaacgcccaa
     8221 gccctgaaca ccctggtgaa gcagctcagc agcaattttg gcgccatcag ctctgtgctg
     8281 aacgatatcc tgtctagact ggaccctcca gaagccgaag tccagatcga tagactgatc
     8341 acaggcagac tgcagtccct gcaaacctac gtgacccaac agctgatcag ggccgctgaa
     8401 ataagagcca gcgccaatct cgccgctacc aagatgtccg agtgtgtgct gggacagtct
     8461 aaacgcgttg acttctgcgg caaaggctat cacctgatga gcttccccca gagcgcgccg
     8521 cacggcgtgg tgttcctgca tgtgacatac gtgcctgccc aagagaagaa tttcacaacc
     8581 gcccctgcca tctgccacga cggcaaggcc cacttcccaa gagagggcgt tttcgtttcc
     8641 aatggcacac actggttcgt gacacaaaga aacttctacg aaccccagat tatcaccacc
     8701 gacaacacct tcgtgagtgg caattgtgac gtggtcatcg gaatcgtgaa caacacagtg
     8761 tacgaccctc tgcaacctga gctggactct tttaaggaag agctggacaa gtactttaaa
     8821 aaccacacca gccccgatgt ggacctgggc gacatcagtg gcattaacgc cagcgtggtg
     8881 aacatccaaa aggaaatcga cagactgaac gaggtggcca agaacctgaa cgagtccctg
     8941 atcgacctgc aggagctcgg caaatacgag cagggatccg gatacatccc cgaggccccc
     9001 agagatggcc aggcctacgt gcggaaggac ggcgagtggg tactgctgag cacattcctg
     9061 ggcagatccg actacaaaga ccatgacggt gattataaag atcatgacat cgattacaag
     9121 gatgacgatg acaagagcgc ctggtcccac ccccagttcg agaagggatc cctggaggtg
     9181 ctgttccagg gcccaggcgg cggcggtagt ggagggggcg gatctggcgg ctcagctgct
     9241 gtgggccagg acacgcagga ggtcatcgtg gtgccacact ccttgccctt taaggtggtg
     9301 gtgatctcag ccatcctggc cctggtggtg ctcaccatca tctcccttat catcctcatc
     9361 atgctttggc agaagaagcc acgttaacgg ccgctcgagt ctagagggcc cgtttaaacc
     9421 cgctgatcag cctcga
//
