LOCUS       sequence-340795-        8815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pWSK29 with S. flexneri 2a wzzBSF gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Murray GL, Attridge SR, Morona R
  TITLE     Altering the length of the lipopolysaccharide O antigen has an 
            impact on the interaction of Salmonella enterica serovar Typhimurium
            with macrophages and complement.
  JOURNAL   J Bacteriol. 2006 Apr;188(7):2735-9. doi: 
            10.1128/JB.188.7.2735-2739.2006.
  PUBMED    16547065
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JB.188.7.2735-2739"; journalName: "J Bacteriol"; date: 
            "2006-04"; volume: "188"; issue: "7"; pages: "2735-9"
COMMENT     Sequence Label: pWSK29-wzzBSF
FEATURES             Location/Qualifiers
     source          1..8815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1228..1245
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1362..1383
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1398..1403,1404..1421,1422..1428)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1436..1452
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1441..1463
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1460..1476
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1460..1476
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1495..1515
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1497..1515
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1552..1568
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1552..1568
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4983..4999)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4984..5000)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(5024..5043)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5025..5043)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5053..5070)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5053..5069)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5062..5084)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5211..5666
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5298..5317)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5508..5529
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5692..5796
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5797..5865,5866..6657)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6015..6034)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(7678..8628)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      join(8676..8815,1..83)
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata
       61 gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg
      121 tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc
      181 accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct
      241 gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg
      301 gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac ccataataca
      361 agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgcgga gtggtgaatc
      421 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
      481 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
      541 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccaa tgatcgaagt
      601 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
      661 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
      721 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
      781 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
      841 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
      901 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcaccct
      961 gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc
     1021 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggc attcgccatt
     1081 caggctgcct cctaccaaga cgacttcaaa tccgacccgt agagatctca catgttcttt
     1141 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     1201 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     1261 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     1321 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     1381 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     1441 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     1501 aaccctcact aaagggaaca aaagctggag ctccaccgcg gtggcggccg ctctagaact
     1561 agtggatccc ccgggctgca ggaattcctc cgcgaaggta aagcccttta cgataatctc
     1621 catccgtcac gtattgtcat cggtgagcgt tcagaacgcg cagaacgttt tgctgcgttg
     1681 ttacaggaag gcgcgattaa gcaaaatatc ccgaccctgt ttaccgactc cactgaagca
     1741 gaagcgatta aacttttcgc taatacctat ctggcgatgc gcgtagcata ctttaatgaa
     1801 ctggatagtt atgcagaaag tttaggtctg aatactcgcc agattatcga aggcgtttgt
     1861 ctcgatccgc gtattggcaa ccattacaac aatccgtcgt ttggttatgg tggttattgt
     1921 ctgccgaaag ataccaagca gttactggcg aactaccagt ctgtgccgaa taacctgatc
     1981 tcggcaattg tcgatgcaaa ccgcaagtgg tgggtattta tcgtctgatt atgaagagcg
     2041 ggtcagataa cttccgtgcg tcttctattc aggggattat gaagcgtatc aaggcgaaag
     2101 gcgttgaagt gatcatctac gagccggtga tgaaagaaga ctcattcttc aactctcgcc
     2161 tggaacgtga tctcgccacc ttcaaacaac aagccgacgt cattatctcc aaccgtatgg
     2221 cagaagagct taaggatgtg gcagataagg tctacacccg cgatctcttt ggcagcgact
     2281 aacatcttgt tatcagggct atttacgccc tgattgtctt ttgttacctc cgcaataatt
     2341 cattattttt atcacttatc ctatagcatt cacggggatt atcgctaaac tatgcggact
     2401 tggaaatttc cgtcagttag ggtaatgatg agagtagaaa ataataatgt ttctgggcaa
     2461 aaccatgacc cggaacagat tgatttgatt gatttactag tgcagttgtg gcgtggcaag
     2521 atgacaatta tcatttccgt cattgtggct attgccctgg ctattggtta tttggcagta
     2581 gcgaaggaga aatggacgtc aacagcaatt atcactcagc ccgacgtggg gcaaattgct
     2641 ggctataaca atgccatgaa tgttatctat ggtcaggctg caccgaaagt atcggatttg
     2701 caggagacgt taattggtcg cttcagttct gccttctctg cattagcaga aacgctggat
     2761 aatcaggaag agccagaaaa acttaccatc gaaccttctg ttaagaacca gcaattacca
     2821 ttgactgttt cttatgttgg gcaaactgca gagggcgcac aaatgaagtt ggcccaatac
     2881 attcagcaag ttgatgataa agtgaatcaa gagctagaaa aggatctcaa ggacaacatt
     2941 gctctgggac ggaaaaactt gcaggactct ttaagaaccc aggaagtggt cgcgcaggag
     3001 cagaaagatc tgcgtatccg tcagattcag gaagcgttgc agtatgcgaa tcaggcgcag
     3061 gtgacaaagc cacaggttca gcagactgaa gatgtgacgc aagatacgtt gttccttcta
     3121 gggagcgaag cgctggagtc gatgattaag catgaagcga ctcgtccgtt ggtgttctca
     3181 ccaaactact atcagacacg tcaaaacctg ttggatattg aaaaattaaa gtttgatgat
     3241 cttgatattc atgcttaccg ctatgtgatg aaaccgacgt tacctattcg tcgcgatagt
     3301 ccgaaaaagg caatcacctt gattctggca gtgcttctgg gcggcatggt tggcgcgggg
     3361 attgtgttgg ggcgtaacgc tctgcgtaat tacaacgcga agtaatatta ttgtgcattt
     3421 aagagaaacg ggcagggtgg tgacaccatg cccgtttttt ttgccggatg cgatgctggc
     3481 gcatcttatc cggcctacgt gtgttgagat aatgtgtagg cacgataagt ttgcgcatcg
     3541 ggcaatggct ccgggtgtga caacaacatc acacctgctc agcccgtaac tcactgatgc
     3601 cgtttatgca ggttctcaat tactgtcgtt aaatccagat cctgatcctg caacagcacc
     3661 agcaggtgat acatcaagtc agatgcctcg ttggtcagct caaagcgatc atgtaccgtt
     3721 gctgccagtg cggtttccac gccttcttcg cccactttct gcgcaatgcg tttagtgccg
     3781 ctggcataca gtttggcggt gtaggaggtt tccgggtcgg catatttacg ttcggcgagc
     3841 agttgttcca gttgatacag gaacagccac tggtgagcgg tgttgccgaa gcagctactg
     3901 gtgcctttgt ggcaggttgg cccgatggga ttcgccagca ccagtaatgt gtcgttgtcg
     3961 cagtccgggg caatacttac cacattgaga aaattgcccg aggtttcgcc tttgatccac
     4021 agtcgctgtt tagtgcgcga gaagaaggtg actttaccgc tttcgatggt tttgtctaag
     4081 gcttccgggt tcatgtagcc cagcattaac acttcgccag ataccgcgtg ttgcacaatc
     4141 accggcatca gaccgtcggt tttttcccag tccagttcgc gacgttgttg ttctgttaac
     4201 atatcctgat ctccacgccc tgtgttgcca ggtacgcttt taattcgcca atattgatta
     4261 tttgtttgtg gaatacggaa gctgccagcg caccgtcaac gtcggcatcg cggaaggctt
     4321 ccaggaagtg ttccatggtg cccgcgccac cggaggcaat cagcgggacg tggcaaactt
     4381 cacgcacttt tttcagctgc ttgaggtcgt aaccgttaca cacgccgtcc tgattcatca
     4441 tgttgaggac gatttctccg gcgccgcgtt tttgcacctc ctctacccag tcgagcgttt
     4501 cccattgagt gacgcgggtg cggctttcat cgccggtata ttgatttaca tgatatttac
     4561 cggtttcggc gtcgtaccag gtatcaatac caaccacaat acactgcacg ccaaagcgat
     4621 cggccaggcg agtaattaat gttgggtctg ccagcgcagg ggagttgatg gaaattttat
     4681 ccgcgccaaa ggaaagaatt ttcgcggcat cttccagaga cttaatccca cccgccacac
     4741 aaaacggaat gtcgatcact tccgccacgc gagataccca gcttttatct accacacggc
     4801 catcgctgga agcggtgata tcgtagaaca ccagttcatc tgcaccttct tcggcgtagc
     4861 gttttgccag tggcacgata tcgccaatga tttcatggtt gcgaaactgt acgcctttca
     4921 ccacctgacc atcacgaacg tcgagacacg ggattatgcg ttttgccagc atgcaagctt
     4981 atcgataccg tcgacctcga gggggggccc ggtacccaat tcgccctata gtgagtcgta
     5041 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     5101 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     5161 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     5221 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     5281 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     5341 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     5401 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     5461 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     5521 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     5581 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     5641 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     5701 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5761 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5821 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5881 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5941 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     6001 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     6061 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     6121 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     6181 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     6241 tttttgcaca acatggggga tcatgtaact cgccttgatc gctgggaacc ggagctgaat
     6301 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     6361 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     6421 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6481 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6541 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6601 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6661 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6721 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6781 tcgttccact gagcgtcaga tctctgttcg ggggttcact gacgctgcgt gactacgatg
     6841 gtcaggttgc ggaggctatg gccctggtac gagcgctgaa caaaatgacg aaagcaggta
     6901 tgcctgaaag cgtgcgtatt gcctgaaaac acaacccgct acgggggaga cttacccgaa
     6961 atctgattta ttcaacaaag ccactttcag tatggatttg ggtgttacgg gggggggttg
     7021 gtgcttaaaa tcgcgctgag gatttctgat ggtgttaagc gggcggtttt gagatgtaaa
     7081 ctcgcccgtt taacataatg gatcttgcgc gcaccgcccg aacaccactc gccacaaaaa
     7141 accgccggaa cgtccaaaag tacgggtttt gctgcccgca aacgggctgt tctggtgttg
     7201 ctagtttgtt atcagaatcg cagatccggc ttcagccggt ttgccggctg aaagcgctat
     7261 ttcttccaga attgccatga ttttttcccc acgggaggcg tcactggctc ccgtgttgtc
     7321 ggcagctttg attcgataag cagcatcgcc tgtttcaggc tgtctatgtg tgactgttga
     7381 gctgtaacaa gttgtctcag gtgttcaatt tcatgttcta gttgctttgt tttactggtt
     7441 tcacctgttc tattaggtgt tacatgctgt tcatctgtta cattgtcgat ctgttcatgg
     7501 tgaacagctt tgaatgcacc aaaaactcgt aaaagctctg atgtatctat cttttttaca
     7561 ccgttttcat ctgtgcatat ggacagtttt ccctttgata tgtaacggtg aacagttgtt
     7621 ctacttttgt ttgttagtct tgatgcttca ctgatagata caagagccat aagaacctca
     7681 gatccttccg tatttagcca gtatgttctc tagtgtggtt cgttgttttt gcgtgagcca
     7741 tgagaacgaa ccattgagat catacttact ttgcatgtca ctcaaaaatt ttgcctcaaa
     7801 actggtgagc tgaatttttg cagttaaagc atcgtgtagt gtttttctta gtccgttatg
     7861 taggtaggaa tctgatgtaa tggttgttgg tattttgtca ccattcattt ttatctggtt
     7921 gttctcaagt tcggttacga gatccatttg tctatctagt tcaacttgga aaatcaacgt
     7981 atcagtcggg cggcctcgct tatcaaccac caatttcata ttgctgtaag tgtttaaatc
     8041 tttacttatt ggtttcaaaa cccattggtt aagcctttta aactcatggt agttattttc
     8101 aagcattaac atgaacttaa attcatcaag gctaatctct atatttgcct tgtgagtttt
     8161 cttttgtgtt agttctttta ataaccactc ataaatcctc atagagtatt tgttttcaaa
     8221 agacttaaca tgttccagat tatattttat gaattttttt aactggaaaa gataaggcaa
     8281 tatctcttca ctaaaaacta attctaattt ttcgcttgag aacttggcat agtttgtcca
     8341 ctggaaaatc tcaaagcctt taaccaaagg attcctgatt tccacagttc tcgtcatcag
     8401 ctctctggtt gctttagcta atacaccata agcattttcc ctactgatgt tcatcatctg
     8461 agcgtattgg ttataagtga acgataccgt ccgttctttc cttgtagggt tttcaatcgt
     8521 ggggttgagt agtgccacac agcataaaat tagcttggtt tcatgctccg ttaagtcata
     8581 gcgactaatc gctagttcat ttgctttgaa aacaactaat tcagacatac atctcaattg
     8641 gtctaggtga ttttaatcac tataccaatt gagatgggct agtcaatgat aattactagt
     8701 ccttttcctt tgagttgtgg gtatctgtaa attctgctag acctttgctg gaaaacttgt
     8761 aaattctgct agaccctctg taaattccgc tagacctttg tgtgtttttt ttgtt
//
