LOCUS       sequence-340809-        4589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pQE30 encoding Wzz mutant  i81.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Papadopoulos M, Morona R
  TITLE     Mutagenesis and chemical cross-linking suggest that Wzz dimer 
            stability and oligomerization affect lipopolysaccharide O-antigen 
            modal chain length control.
  JOURNAL   J Bacteriol. 2010 Jul;192(13):3385-93. doi: 10.1128/JB.01134-09. 
            Epub 2010 May 7.
  PUBMED    20453100
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JB.01134-09"; journalName: "J Bacteriol"; date: "2010-07"; 
            volume: "192"; issue: "13"; pages: "3385-93"
COMMENT     Sequence Label: pMPRMA49
FEATURES             Location/Qualifiers
     source          1..4589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      52..138
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(185..207)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    293..433
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     348..367
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(448..465)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(619..1207)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(699..718)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1378..2169,2170..2238))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2001..2020
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2239..2343)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2411..2429
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    2473..2489
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2508..2519
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2538..2555
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3779..3873
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             3917..4576
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3983..4002)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gttattggtg cccttaaacg cctggggtaa tgactctcta gcttgaggca tcaaataaaa
       61 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
      121 ctcctgagta ggacaaatcc gccctctaga gctgcctcgc gcgtttcggt gatgacggtg
      181 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
      241 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
      301 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
      361 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
      421 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
      481 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
      541 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
      601 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
      661 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      721 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      781 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      841 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      901 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
      961 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1021 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     1081 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     1141 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     1201 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     1261 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     1321 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     1381 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     1441 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     1501 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     1561 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     1621 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     1681 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     1741 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     1801 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     1861 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     1921 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     1981 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     2041 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     2101 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     2161 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     2221 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     2281 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     2341 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     2401 aacctataaa aataggcgta tcacgaggcc ctttcgtctt cacctcgaga aatcataaaa
     2461 aatttatttg ctttgtgagc ggataacaat ttcacacaga attcattaaa gaggagaaat
     2521 taactatgag aggatcgcat caccatcacc atcacggatc cgcatgcgag ctcagagtag
     2581 aaaataataa tgtttctggg caaaaccatg acccggaaca gattgatttg attgatttac
     2641 tagtgcagtt gtggcgtggc aagatgacaa ttatcatttc cgtcattgtg gctattgccc
     2701 tggctattgg ttatttggca gtagcgaagg agaaatggac gtcaacagca attatcactc
     2761 agcccgacgt ggggcaaatt gctggctata acaatgccat gaatgttatc tattgcggcc
     2821 gcatctatgg tcaggctgca ccgaaagtat cggatttgca ggagacgtta attggtcgct
     2881 tcagttctgc cttctctgca ttagcagaaa cgctggataa tcaggaagag ccagaaaaac
     2941 ttaccatcga accttctgtt aagaaccagc aattaccatt gactgtttct tatgttgggc
     3001 aaactgcaga gggcgcacaa atgaagttgg cccaatacat tcagcaagtt gatgataaag
     3061 tgaatcaaga gctagaaaag gatctcaagg acaacattgc tctgggacgg aaaaacttgc
     3121 aggactcttt aagaacccag gaagtggtcg cgcaggagca gaaagatctg cgtatccgtc
     3181 agattcagga agcgttgcag tatgcgaatc aggcgcaggt gacaaagcca caggttcagc
     3241 agactgaaga tgtgacgcaa gatacgttgt tccttctagg gagcgaagcg ctggagtcga
     3301 tgattaagca tgaagcgact cgtccgttgg tgttctcacc aaactactat cagacacgtc
     3361 aaaacctgtt ggatattgaa aaattaaagt ttgatgatct tgatattcat gcttaccgct
     3421 atgtgatgaa accgacgtta cctattcgtc gcgatagtcc gaaaaaggca atcaccttga
     3481 ttctggcagt gcttctgggc ggcatggttg gcgcggggat tgtgttgggg cgtaacgctc
     3541 tgcgtaatta caacgcgaag taatattatt gtgcatttaa gagaaacggg cagggtggtg
     3601 acaccatgcc cgtttttttt gccggatgcg atgctggcgc atcttatccg gcctacgtgt
     3661 gttgagataa tgtgtaggca cgataagttt gcgcatcggg caatggctcc gggtgtgaca
     3721 acaacatcac acctgctccc cgggtcgacc tgcagccaag cttaattagc tgagcttgga
     3781 ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc
     3841 ggttgccgcc gggcgttttt tattggtgag aatccaagct agcttggcga gattttcagg
     3901 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     3961 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     4021 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     4081 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aatttcgtat
     4141 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     4201 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     4261 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     4321 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
     4381 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     4441 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     4501 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     4561 gcagggcggg gcgtaatttt tttaaggca
//
