LOCUS       sequence-340811-        7224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pWSK29 with Salmonella typhimurium wzzBST gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Murray GL, Attridge SR, Morona R
  TITLE     Altering the length of the lipopolysaccharide O antigen has an 
            impact on the interaction of Salmonella enterica serovar Typhimurium
            with macrophages and complement.
  JOURNAL   J Bacteriol. 2006 Apr;188(7):2735-9. doi: 
            10.1128/JB.188.7.2735-2739.2006.
  PUBMED    16547065
REFERENCE   2  (bases 1 to 7224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JB.188.7.2735-2739"; journalName: "J Bacteriol"; date: 
            "2006-04"; volume: "188"; issue: "7"; pages: "2735-9"
COMMENT     Sequence Label: pWSK29-wzzBST
FEATURES             Location/Qualifiers
     source          1..7224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(536..1327,1328..1396))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1159..1178
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1397..1501)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1527..1982)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1664..1685)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1876..1895
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2109..2131
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2123..2140
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2124..2140
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2150..2169
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2150..2168
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4034..4050)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4034..4050)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4087..4107)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4087..4105)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4126..4142)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4126..4142)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4139..4161)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4150..4166
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4174..4180,4181..4198,4199..4204))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4219..4240
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4357..4374)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      5519..5741
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             5789..6739
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 gaacagcccg tttgcgggca gcaaaacccg tacttttgga cgttccggcg gttttttgtg
       61 gcgagtggtg ttcgggcggt gcgcgcaaga tccattatgt taaacgggcg agtttacatc
      121 tcaaaaccgc ccgcttaaca ccatcagaaa tcctcagcgc gattttaagc accaaccccc
      181 ccccgtaaca cccaaatcca tactgaaagt ggctttgttg aataaatcag atttcgggta
      241 agtctccccc gtagcgggtt gtgttttcag gcaatacgca cgctttcagg catacctgct
      301 ttcgtcattt tgttcagcgc tcgtaccagg gccatagcct ccgcaacctg accatcgtag
      361 tcacgcagcg tcagtgaacc cccgaacaga gatctgacgc tcagtggaac gaaaactcac
      421 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
      481 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
      541 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
      601 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
      661 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
      721 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
      781 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
      841 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
      901 ccggttccca gcgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
      961 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     1021 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     1081 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     1141 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     1201 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     1261 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     1321 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     1381 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     1441 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     1501 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
     1561 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     1621 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     1681 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     1741 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     1801 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     1861 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     1921 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     1981 gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     2041 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     2101 agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc
     2161 actatagggc gaattgggta ccaaacgtat tgcgcaaaaa gttggcgaag aaggtgtgga
     2221 aaccgcgctg gcggccaccg tcaatgatcg ttttgaatta accaatgaag cgtctgattt
     2281 gatgtaccac ctgctggtgc tgttgcagga tcaggatctc aacctcacca ccgtgatcga
     2341 caatcttcgt aagcgtcatc aataagcgta aaaaaaccgg gcaatgcccg gttttttaat
     2401 gagaaatttt acctgtcgta gccgaccacc atccggcaaa gaagcttaca aggcttttgg
     2461 cttatagcta cgtagcgcat tgcgtcccag cacaatcccg gcaccgatca tcccacccag
     2521 caatacagcc agcacaaggg taatggctgt tttcgggcta tcgcgacgga ccggcagcgt
     2581 cggcttcatc acataacgat agacgtgcac cgtatcggca gtcactttca gatttttaat
     2641 gtccagcagt gtctgcttcg tctggtaata ggccggagaa aagaccagtg gacgcgtcgc
     2701 ttcgttctgt atcatcgatt ttagcgcatc gctccccaac aggaacatcg tgtcctgggt
     2761 aacatcctgg gtttgctgaa tctgcggctg cgtgattttg gcctcatccg catagcgcaa
     2821 cgcttcttcg atttgctcaa tacgcagatc tttttgctcc tgcgccacaa cttcctgcgt
     2881 ctcaagggac tcctgcaacg ttttggtttg cagcgtgatg ttatctttca ggtcaacttc
     2941 cagttcctta gcgacctctt catccacctg ttggatatat tccgccagcc gacgctgcgc
     3001 cccttcagcg gtagtactca cataagaaac cgagagtggc agcgcctgcc cttttaccga
     3061 ctgttcaatg gtaagctttt cccgctcttt ctgattatcc agcacttccg ataatgcgga
     3121 aaacgcagag ctaaagcggc ttataaaatt cgcctgcact tcagagattt tgggggcatt
     3181 cccaccatac aagacgttga gcgcgttggt ataggtggca acctgcgcgg catcaggttg
     3241 ggtaataatc gccgtggatg tccatttttc tttggctatc atcaggtagc ctacagccag
     3301 caaaatggcg ataataacgg ctacaataat ggtcatcttc ccacgccata actgtagcaa
     3361 taactcaatc aaatcaatct gttccggatc gttcccacgc ccggaagacg tattactatc
     3421 cactgtcata gataccctaa ctaaaaaaag gatgaagctg gagacagtgt agccataaag
     3481 caaatatatg ccataggatt aatgataaaa atcagacatt atttccattt ggtcaataaa
     3541 tcatcgggac tcatcagccc cgattgcctt attaatcact accgaaaaga tcgcgggtat
     3601 acactttttc agcgacatcc aaaagctctg ccgccatcct gttggaaata atgacatccg
     3661 cctgctgttt aaagcagtgc aaatcacgtt caagacgtga gttaaagaaa gtatcttctt
     3721 ccattaccgg ctcatagatg atgacctcta cgcctttcgc tttaatgcgc ttcataatcc
     3781 cctgaatcga tgaggcgcgg aagttatcgg agccactctt cataatcagg cgataaatgc
     3841 ccaccacttt aggctttctg gcgaggatag cgtcggcaat aaaatcttta cgcgtacggc
     3901 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
     3961 taatgcagga gtcgcataag ggagagcgtc gacggtatcg ataagcttga tatcgaattc
     4021 ctgcagcccg ggggatccac tagttctaga gcggccgcca ccgcggtgga gctccagctt
     4081 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     4141 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     4201 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     4261 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     4321 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     4381 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4441 agaatcaggg gataacgcag gaaagaacat gtgagatctc tacgggtcgg atttgaagtc
     4501 gtcttggtag gaggcagcct gaatggcgaa tgccgatgcc cttgagagcc ttcaacccag
     4561 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
     4621 ttatcatgca actcgtagga cagggtgccg gcagcgctct gggtcatttt cggcgaggac
     4681 cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac
     4741 gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc
     4801 attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga
     4861 ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg
     4921 ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg
     4981 ctcgcggctc ttaccagcct aacttcgatc attggaccgc tgatcgtcac ggcgatttat
     5041 gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt
     5101 gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctgaatggaa
     5161 gccggcggca cctcgctaac ggattcacca ctccgcagac ccgccataaa acgccctgag
     5221 aagcccgtga cgggcttttc ttgtattatg ggtagtttcc ttgcatgaat ccataaaagg
     5281 cgcctgtagt gccatttacc cccattcact gccagagccg tgagcgcagc gaactgaatg
     5341 tcacgaaaaa gacagcgact caggtgcctg atggtcggag acaaaaggaa tattcagcga
     5401 tttgcccgag cttgcgaggg tgctacttaa gcctttaggg ttttaaggtc tgttttgtag
     5461 aggagcaaac agcgtttgcg acatcctttt gtaatactgc ggaactgact aaagtagtga
     5521 gttatacaca gggctgggat ctattctttt tatctttttt tattctttct ttattctata
     5581 aattataacc acttgaatat aaacaaaaaa aacacacaaa ggtctagcgg aatttacaga
     5641 gggtctagca gaatttacaa gttttccagc aaaggtctag cagaatttac agatacccac
     5701 aactcaaagg aaaaggacta gtaattatca ttgactagcc catctcaatt ggtatagtga
     5761 ttaaaatcac ctagaccaat tgagatgtat gtctgaatta gttgttttca aagcaaatga
     5821 actagcgatt agtcgctatg acttaacgga gcatgaaacc aagctaattt tatgctgtgt
     5881 ggcactactc aaccccacga ttgaaaaccc tacaaggaaa gaacggacgg tatcgttcac
     5941 ttataaccaa tacgctcaga tgatgaacat cagtagggaa aatgcttatg gtgtattagc
     6001 taaagcaacc agagagctga tgacgagaac tgtggaaatc aggaatcctt tggttaaagg
     6061 ctttgagatt ttccagtgga caaactatgc caagttctca agcgaaaaat tagaattagt
     6121 ttttagtgaa gagatattgc cttatctttt ccagttaaaa aaattcataa aatataatct
     6181 ggaacatgtt aagtcttttg aaaacaaata ctctatgagg atttatgagt ggttattaaa
     6241 agaactaaca caaaagaaaa ctcacaaggc aaatatagag attagccttg atgaatttaa
     6301 gttcatgtta atgcttgaaa ataactacca tgagtttaaa aggcttaacc aatgggtttt
     6361 gaaaccaata agtaaagatt taaacactta cagcaatatg aaattggtgg ttgataagcg
     6421 aggccgcccg actgatacgt tgattttcca agttgaacta gatagacaaa tggatctcgt
     6481 aaccgaactt gagaacaacc agataaaaat gaatggtgac aaaataccaa caaccattac
     6541 atcagattcc tacctacata acggactaag aaaaacacta cacgatgctt taactgcaaa
     6601 aattcagctc accagttttg aggcaaaatt tttgagtgac atgcaaagta agtatgatct
     6661 caatggttcg ttctcatggc tcacgcaaaa acaacgaacc acactagaga acatactggc
     6721 taaatacgga aggatctgag gttcttatgg ctcttgtatc tatcagtgaa gcatcaagac
     6781 taacaaacaa aagtagaaca actgttcacc gttacatatc aaagggaaaa ctgtccatat
     6841 gcacagatga aaacggtgta aaaaagatag atacatcaga gcttttacga gtttttggtg
     6901 cattcaaagc tgttcaccat gaacagatcg acaatgtaac agatgaacag catgtaacac
     6961 ctaatagaac aggtgaaacc agtaaaacaa agcaactaga acatgaaatt gaacacctga
     7021 gacaacttgt tacagctcaa cagtcacaca tagacagcct gaaacaggcg atgctgctta
     7081 tcgaatcaaa gctgccgaca acacgggagc cagtgacgcc tcccgtgggg aaaaaatcat
     7141 ggcaattctg gaagaaatag cgctttcagc cggcaaaccg gctgaagccg gatctgcgat
     7201 tctgataaca aactagcaac acca
//
