LOCUS       sequence-340812-        7010 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pWSK29 with Salmonella typhimurium fepEST gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7010)
  AUTHORS   Murray GL, Attridge SR, Morona R
  TITLE     Altering the length of the lipopolysaccharide O antigen has an 
            impact on the interaction of Salmonella enterica serovar Typhimurium
            with macrophages and complement.
  JOURNAL   J Bacteriol. 2006 Apr;188(7):2735-9. doi: 
            10.1128/JB.188.7.2735-2739.2006.
  PUBMED    16547065
REFERENCE   2  (bases 1 to 7010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JB.188.7.2735-2739"; journalName: "J Bacteriol"; date: 
            "2006-04"; volume: "188"; issue: "7"; pages: "2735-9"
COMMENT     Sequence Label: pWSK29-fepEST
FEATURES             Location/Qualifiers
     source          1..7010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(174..965,966..1034))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     797..816
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1035..1139)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1165..1620)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1302..1323)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1514..1533
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1747..1769
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1761..1778
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1762..1778
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1788..1807
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1788..1806
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3458..3474)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3458..3474)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3511..3531)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3511..3529)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3550..3566)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3550..3566)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3563..3585)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3574..3590
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3598..3604,3605..3622,3623..3628))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3643..3664
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3781..3798)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      4943..5165
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             5213..6163
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 acgcagcgtc agtgaacccc cgaacagaga tctgacgctc agtggaacga aaactcacgt
       61 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
      121 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
      181 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
      241 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
      301 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
      361 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
      421 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
      481 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
      541 ggttcccagc gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
      601 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
      661 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
      721 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
      781 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
      841 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
      901 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
      961 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     1021 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     1081 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     1141 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt
     1201 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     1261 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     1321 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     1381 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     1441 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     1501 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     1561 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     1621 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     1681 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     1741 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
     1801 tatagggcga attgggtacc gggccccccc tcgagccatc atcagcgaac ttattaataa
     1861 ataacgggcg ttgtttctgc ctttaacaaa ttaaatcctg aaacccataa taattactaa
     1921 ttattatggg ttttttattg caactattaa ttcttttaac ataagtgata catgctacag
     1981 gcaagtttaa ttccgaatat ttagcttttc gggcactggc gcgtaaagat tgtttcggat
     2041 aattctgact tgctgttaga atctctgaca ggaatgtgtt ctttcattgg ataaattttt
     2101 caggtcatac ggcatgccat ctcttaatgt aaaacaagaa aaaaatcagt catttgcagg
     2161 ttattcactg ccgcccgcca acagtcatga aatcgatttg tttagcctta tagaggtgtt
     2221 atggcaggcg aaacgtcgta ttcttgctac cgttttcgcc tttgcgtgcg tggggttgct
     2281 tctgtccttt ctgctgccgc aaaaatggac cagccaggcg attgtcacac cggcggagtc
     2341 ggtacagtgg caggggctgg agagaacgtt gaccgcgctg cgcgtgttgg atatggaggt
     2401 aagcgttgat cggggcagcg tatttaatct gtttattaaa aagtttagct cgccctcgct
     2461 gctggaagaa tatcttcgtt cttctccgta tgtcatggat caattaaaag gcgcgcaaat
     2521 agacgagcag gatcttcacc gggcgattgt cctgctgagc gaaaaaatga aagcggtgga
     2581 cagtaatgtc agcaagaaaa atgaaacgtc gttattcacg tcgtggacat tgagttttac
     2641 cgcgccgacg cgggaagaag cgcaaaaagt gctggctggc tatattcagt acatctccga
     2701 tatcgtcgtg aaagagacgc tggaaaatat tcgtaaccag ctggaaatca aaacccgcta
     2761 tgagcaggaa aagctggcga tggatcgggt gcgtctcaaa aatcagcttg atgccaatat
     2821 tcaacgtctt cattattcgc tggaaatcgc caacgccgcc ggtattaaga gaccggttta
     2881 cagcaatggt caggcggtaa aagatgatcc ggatttttct atttctctcg gcgcggatgg
     2941 tatttcccgc aaactggaaa ttgaaaaagg ggtaacggac gtggccgaga tcgacggtga
     3001 tttgcgtaac cgtcaatacc atgttgaaca actggcggca atgaatgtga gtgacgtgaa
     3061 gtttaccccg tttaaatatc aactgtcgcc gtctctgcca gtgaaaaaag atggcccggg
     3121 taaagccatc attattatcc tggcggcgtt gattggcggt atgatggcct gcggcggcgt
     3181 attactgcat cacgcgatgg tctcgcgtaa aatggaaaac gcgctggcga tagatgaacg
     3241 gttagtctga tttacccgaa aagccggata gcgatatccg gctttgtcat cagtgtgggc
     3301 gactatcgcc gccccagcgg taccaccagc ggcgtcccgg caaccggatc gtcgataatc
     3361 atacagcgca gcccatagat tttttcgata agctctgcgg taacaatctc tttcggcgcg
     3421 ccctgtgcga caatattacc ttcgcgatgc aatcaaggat ccactagttc tagagcggcc
     3481 gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     3541 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     3601 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     3661 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     3721 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     3781 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3841 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgaga
     3901 tctctacggg tcggatttga agtcgtcttg gtaggaggca gcctgaatgg cgaatgccga
     3961 tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg
     4021 tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacagggt gccggcagcg
     4081 ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg
     4141 cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc
     4201 aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac
     4261 gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct
     4321 tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac
     4381 catcagggac agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcattgga
     4441 ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg
     4501 attgtaggcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc
     4561 cgggccacct cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccgc
     4621 agacccgcca taaaacgccc tgagaagccc gtgacgggct tttcttgtat tatgggtagt
     4681 ttccttgcat gaatccataa aaggcgcctg tagtgccatt tacccccatt cactgccaga
     4741 gccgtgagcg cagcgaactg aatgtcacga aaaagacagc gactcaggtg cctgatggtc
     4801 ggagacaaaa ggaatattca gcgatttgcc cgagcttgcg agggtgctac ttaagccttt
     4861 agggttttaa ggtctgtttt gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata
     4921 ctgcggaact gactaaagta gtgagttata cacagggctg ggatctattc tttttatctt
     4981 tttttattct ttctttattc tataaattat aaccacttga atataaacaa aaaaaacaca
     5041 caaaggtcta gcggaattta cagagggtct agcagaattt acaagttttc cagcaaaggt
     5101 ctagcagaat ttacagatac ccacaactca aaggaaaagg actagtaatt atcattgact
     5161 agcccatctc aattggtata gtgattaaaa tcacctagac caattgagat gtatgtctga
     5221 attagttgtt ttcaaagcaa atgaactagc gattagtcgc tatgacttaa cggagcatga
     5281 aaccaagcta attttatgct gtgtggcact actcaacccc acgattgaaa accctacaag
     5341 gaaagaacgg acggtatcgt tcacttataa ccaatacgct cagatgatga acatcagtag
     5401 ggaaaatgct tatggtgtat tagctaaagc aaccagagag ctgatgacga gaactgtgga
     5461 aatcaggaat cctttggtta aaggctttga gattttccag tggacaaact atgccaagtt
     5521 ctcaagcgaa aaattagaat tagtttttag tgaagagata ttgccttatc ttttccagtt
     5581 aaaaaaattc ataaaatata atctggaaca tgttaagtct tttgaaaaca aatactctat
     5641 gaggatttat gagtggttat taaaagaact aacacaaaag aaaactcaca aggcaaatat
     5701 agagattagc cttgatgaat ttaagttcat gttaatgctt gaaaataact accatgagtt
     5761 taaaaggctt aaccaatggg ttttgaaacc aataagtaaa gatttaaaca cttacagcaa
     5821 tatgaaattg gtggttgata agcgaggccg cccgactgat acgttgattt tccaagttga
     5881 actagataga caaatggatc tcgtaaccga acttgagaac aaccagataa aaatgaatgg
     5941 tgacaaaata ccaacaacca ttacatcaga ttcctaccta cataacggac taagaaaaac
     6001 actacacgat gctttaactg caaaaattca gctcaccagt tttgaggcaa aatttttgag
     6061 tgacatgcaa agtaagtatg atctcaatgg ttcgttctca tggctcacgc aaaaacaacg
     6121 aaccacacta gagaacatac tggctaaata cggaaggatc tgaggttctt atggctcttg
     6181 tatctatcag tgaagcatca agactaacaa acaaaagtag aacaactgtt caccgttaca
     6241 tatcaaaggg aaaactgtcc atatgcacag atgaaaacgg tgtaaaaaag atagatacat
     6301 cagagctttt acgagttttt ggtgcattca aagctgttca ccatgaacag atcgacaatg
     6361 taacagatga acagcatgta acacctaata gaacaggtga aaccagtaaa acaaagcaac
     6421 tagaacatga aattgaacac ctgagacaac ttgttacagc tcaacagtca cacatagaca
     6481 gcctgaaaca ggcgatgctg cttatcgaat caaagctgcc gacaacacgg gagccagtga
     6541 cgcctcccgt ggggaaaaaa tcatggcaat tctggaagaa atagcgcttt cagccggcaa
     6601 accggctgaa gccggatctg cgattctgat aacaaactag caacaccaga acagcccgtt
     6661 tgcgggcagc aaaacccgta cttttggacg ttccggcggt tttttgtggc gagtggtgtt
     6721 cgggcggtgc gcgcaagatc cattatgtta aacgggcgag tttacatctc aaaaccgccc
     6781 gcttaacacc atcagaaatc ctcagcgcga ttttaagcac caaccccccc ccgtaacacc
     6841 caaatccata ctgaaagtgg ctttgttgaa taaatcagat ttcgggtaag tctcccccgt
     6901 agcgggttgt gttttcaggc aatacgcacg ctttcaggca tacctgcttt cgtcattttg
     6961 ttcagcgctc gtaccagggc catagcctcc gcaacctgac catcgtagtc
//
