LOCUS       sequence-340818-        5988 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses BMP-2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5988)
  AUTHORS   Aihara S, Fujimoto S, Sakaguchi R, Imai T
  TITLE     BMPR-2 gates activity-dependent stabilization of primary dendrites 
            during mitral cell remodeling.
  JOURNAL   Cell Rep. 2021 Jun 22;35(12):109276. doi: 
            10.1016/j.celrep.2021.109276.
  PUBMED    34161760
REFERENCE   2  (bases 1 to 5988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-06-22"; volume: "35(12):109276. doi"; pages: " 
            10.1016/j.celrep.2021.109276"
COMMENT     Sequence Label: pCAG-Bmp2
FEATURES             Location/Qualifiers
     source          1..5988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          663..1679
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1603..1625
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1687..1706
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1734..1743
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1738..1747
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3010..3029)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3075..3130
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3129..3148)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3491..3507)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3491..3507)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3504..3526)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3515..3531
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3539..3545,3546..3563,3564..3569))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3584..3605
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3664..3859
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3710..3845
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3865..3999
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3902..3921)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3956..3975
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4067..4084)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4238..4826)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4318..4337)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4997..5788,5789..5857))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5620..5639
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5858..5962)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggnsgcg cgccrggsgg ggsggggsgg ggsgrggggs
      541 ggggsggggs gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcgccacca
     1741 tggtggccgg gacccgctgt cttctagtgt tgctgcttcc ccaggtcctc ctgggcggcg
     1801 cggccggcct cattccagag ctgggccgca agaagttcgc cgcggcatcc agccgaccct
     1861 tgtcccggcc ttcggaagac gtcctcagcg aatttgagtt gaggctgctc agcatgtttg
     1921 gcctgaagca gagacccacc cccagcaagg acgtcgtggt gcccccctat atgctagatc
     1981 tgtaccgcag gcactcaggc cagccaggag cgcccgcccc agaccaccgg ctggagaggg
     2041 cagccagccg cgccaacacc gtgcgcagct tccatcacga agaagccgtg gaggaacttc
     2101 cagagatgag tgggaaaacg gcccggcgct tcttcttcaa tttaagttct gtccccagtg
     2161 acgagtttct cacatctgca gaactccaga tcttccggga acagatacag gaagctttgg
     2221 gaaacagtag tttccagcac cgaattaata tttatgaaat tataaagcct gcagcagcca
     2281 acttgaaatt tcctgtgacc agactattgg acaccaggtt agtgaatcag aacacaagtc
     2341 agtgggagag cttcgacgtc accccagctg tgatgcggtg gaccacacag ggacacacca
     2401 accatgggtt tgtggtggaa gtggcccatt tagaggagaa cccaggtgtc tccaagagac
     2461 atgtgaggat tagcaggtct ttgcaccaag atgaacacag ctggtcacag ataaggccat
     2521 tgctagtgac ttttggacat gatggaaaag gacatccgct ccacaaacga gaaaagcgtc
     2581 aagccaaaca caaacagcgg aagcgcctca agtccagctg caagagacac cctttgtatg
     2641 tggacttcag tgatgtgggg tggaatgact ggatcgtggc acctccgggc tatcatgcct
     2701 tttactgcca tggggagtgt ccttttcccc ttgctgacca cctgaactcc actaaccatg
     2761 ccatagtgca gactctggtg aactctgtga attccaaaat ccctaaggca tgctgtgtcc
     2821 ccacagagct cagcgcaatc tccatgttgt acctagatga aaatgaaaag gttgtgctaa
     2881 aaaattatca ggacatggtt gtggagggct gcgggtgtcg ttaggcggcc gcactcctca
     2941 ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata
     3001 ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
     3061 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     3121 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
     3181 tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct
     3241 ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa
     3301 agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac
     3361 atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct cctgactact
     3421 cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttgg
     3481 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     3541 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     3601 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga
     3661 tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     3721 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     3781 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     3841 aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa
     3901 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3961 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc
     4021 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     4081 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     4141 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4201 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4261 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4321 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4381 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4441 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4501 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4561 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4621 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     4681 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4741 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4801 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     4861 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     4921 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     4981 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5041 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5101 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     5161 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     5221 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5281 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     5341 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5401 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5461 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5521 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5581 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5641 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5701 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5761 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     5821 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     5881 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     5941 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctg
//
