LOCUS       sequence-340822-       10607 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pQE30 encoding Wzz mutant  i32.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10607)
  AUTHORS   Papadopoulos M, Morona R
  TITLE     Mutagenesis and chemical cross-linking suggest that Wzz dimer 
            stability and oligomerization affect lipopolysaccharide O-antigen 
            modal chain length control.
  JOURNAL   J Bacteriol. 2010 Jul;192(13):3385-93. doi: 10.1128/JB.01134-09. 
            Epub 2010 May 7.
  PUBMED    20453100
REFERENCE   2  (bases 1 to 10607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JB.01134-09"; journalName: "J Bacteriol"; date: "2010-07"; 
            volume: "192"; issue: "13"; pages: "3385-93"
COMMENT     Sequence Label: pMPRMA45
FEATURES             Location/Qualifiers
     source          1..10607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(68..90)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    176..316
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     231..250
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(331..348)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(502..1090)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(582..601)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1261..2052,2053..2121))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1884..1903
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2122..2226)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2294..2312
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(2336..2350,2351..2356,2357..2373,2374..2380)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    2356..2372
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2388..2404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2423..2434
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2453..2470
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      9680..9774
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             9818..10477
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(9884..9903)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      join(10542..10607,1..21)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gctctcctga gtaggacaaa tccgccctct agagctgcct cgcgcgtttc ggtgatgacg
       61 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
      121 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
      181 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
      241 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
      301 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
      361 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
      421 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
      481 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
      541 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
      601 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
      661 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
      721 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
      781 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
      841 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
      901 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
      961 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     1021 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     1081 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     1141 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     1201 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     1261 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     1321 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     1381 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     1441 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     1501 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     1561 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     1621 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     1681 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     1741 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     1801 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     1861 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     1921 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     1981 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     2041 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     2101 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     2161 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     2221 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     2281 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcacctcg agaaatcata
     2341 aaaaatttat ttgctttgtg agcggataac aattataata gattcaattg tgagcggata
     2401 acaatttcac acagaattca ttaaagagga gaaattaact atgagaggat cgcatcacca
     2461 tcaccatcac ggatccgcat gcgagctcag agtagaaaat aataatgttt ctgggcaaaa
     2521 ccatgacccg gaacagattg atttgattga tttactagtg cagttgtggc gtggcaagat
     2581 gactgcggcc gcaatgacaa ttatcatttc cgtcattgtg gctattgccc tggctattgg
     2641 ttatttggca gtagcgaagg agaaatggac gtcaacagca attatcactc agcccgacgt
     2701 ggggcaaatt gctggctata acaatgccat gaatgttatc tatggtcagg ctgcaccgaa
     2761 agtatcggat ttgcaggaga cgttaattgg tcgcttcagt tctgccttct ctgcattagc
     2821 agaaacgctg gataatcagg aagagccaga aaaacttacc atcgaacctt ctgttaagaa
     2881 ccagcaatta ccattgactg tttcttatgt tgggcaaact gcagagggcg cacaaatgaa
     2941 gttggcccaa tacattcagc aagttgatga tggggtttga gggccaatgg aacgaaaacg
     3001 tacgttaagg agataattcg ttgtttatat ttaaatttag agctctcagt tcccctttta
     3061 aaatatcctc tggcaacgtg aatgtataat ggcccaacat attgatatgc ccgtgcatca
     3121 ggggagatag ccgagcgata tcttcatcta taatttcttc gccattacgg cgcatccagc
     3181 tcaacgcttc ctccatatag agcgtgttcc acagaaccac tgcattagta accaggccca
     3241 gcgcccccag ttgatcttcc tgcccttcac gataacgctt tctgatctct ccgcgttgtc
     3301 cgtaacaaat cgcacgagcc acagcgtggc ggccttctcc tcgattaagc tgcgtcagga
     3361 tccgccgacg ataatcttca tcatcaatat aattgaggag atatagcgtt ttgtttacac
     3421 gccctacttc cataattgcc tgtgccagtc ctgatgggcg cgagcttttc agtaaagagc
     3481 gaatgagttc tgacgcatga attgtaccca acttcagcga accagcggtt cgcatcatct
     3541 catcccactg actctccgct tttgacagat ctgcatatcc tcgggccaac ttatccagta
     3601 ctccgtagtt tgccgattta ttcacccgcc agaacaccgc ctcacctgca tcggcaagcc
     3661 ggggggaaaa ctgatacccc agtagccaga acagaccgaa aataatatcg ctgctacccg
     3721 cagtgtctgt catgatttca actggattca gccctgtctg ctgctcaaga agtccttcca
     3781 gtacaaaaat cgaatcccgt aatgtaccgg gtaccacaat gccatggaac ccagagtact
     3841 gatcagatac gaagttatac caggtgatgc ctcgtccaga accaaaatat tttctgttag
     3901 atcctgagtt gatggtcttc actggtgtga caaagcgcat gccgtcagct gatgccactt
     3961 ctccacctcc ccaacgacca gcaagctcca gtgtggactg aaaatcaacc aggcgggcat
     4021 tggcgctgac cagcgtttct gcacgaaggt aattctgttt cacccaactg agccgatggc
     4081 gggtcagtgc tggtatattg tgctttatca gcggttccag tccgatatta caggcttcag
     4141 ccatcaatac cgcacataaa ctgatgtgca aatcttgcgc tcgagcacca gattcactga
     4201 catgcgcaaa ctcatgtgta aatcctgtct gggcatctat ctcaagtaac agttccgtta
     4261 aatctaccgg tgggagtagc tgtttgatcc gattatttag acgaagcaat gatggtggct
     4321 cttcctgttt ctccagacaa ctgatagtca gggatggata tttaccttca ttacagatat
     4381 gaacttccgc attcttttca aatcgtgatg ccacggcttt ccaggtctca tccagctgaa
     4441 tagccagttg ttgcacacct ttacgtccat cgacaggatg tcccagtgcc cgacagacag
     4501 gaatacgctg agtctgccac tcttcacctt gcaacaactt ctcgcgagga tctccccagc
     4561 gatcactgtt ttcaagccag atgtcccggc ggcgcagtgc atcctgaagg cgttccagca
     4621 aacatagtga ataacctgca cgctgtatcc gtccctccgc atcgtatacg aggcgtttcc
     4681 agggaccggt gataatatgt tcaggcgcat catcaaggat gcgctttttc gaaccattca
     4741 gttctgccag ataatgaatc gcagccagta catgttcacc tgccggtgcc gcacggaaat
     4801 gcaggtcccg caacaccgcc ggaagaaaac gtttaacccg accgtactgc tcaaccattt
     4861 cgtcatggaa attattgttc tgtggacgag caagttcatt aaccttgctt acagattctg
     4921 ccagtctgtt tttgggtacg cacttgaaga taacctgcct gagatctggg acatctgtat
     4981 tatcatccag caacaatgca catgcccgcg ccagtaacaa tgcggcctga tcaagatctt
     5041 tcagtgtcct gagtcttttt ttttgcccgg ttttctttgc ttcgcggata atgtccagaa
     5101 ttagcatatc aagcacatca acggcatcgt ctaatgccgt tatttcctgt gctttaacga
     5161 atgcagtaag tacagcaagc tttctctgct gtggcattcg agcgatatat tttaccgacg
     5221 ccatgccagc ataacgagcc agattacgca gttgaatggc aggcagaccg gaaaagttca
     5281 gtcgggaaaa ctcaagactt cgtaatcgga tatatcgttc cagcgcttca ttaaacgcag
     5341 gtccactgac tgtaacaggg ccttttttca actgttccag tgctgataca cgctgacctt
     5401 ccggaatgac cagaagctcc atcacttgag ctgcctgcca tttgttcggc agtgcggcca
     5461 gctttttcca cagccgctga tttgcccttt cacgaatttc actgatgaga cgtactagtg
     5521 tggttgctcc gggcagtaat accttatttt gaagcaacca tgcagtggca aaatcaaaca
     5581 tcagacccgg tcgctcatta ctgagccacg cccgggtata tagcagacgc ttcaggcgga
     5641 aagaccatgg aaaatcacca aattcatgat agccgtaata ttccttaatt aatgcagtat
     5701 gttctctaag ggtagtgtcc cgttcagcat agcgggagag aacttctgga cggtggatat
     5761 taagctgtac cgcgacaaaa tgttgaacac caggcagaac ctgagttaaa tccgtcagaa
     5821 atgttcccag aaaacgggct gtggtgagct gaagcgcaat gcccagccta ttatgtttgc
     5881 cccgtcgttg gttaatgaag gccagatccc gttcatcaag atgaaaatag cgcgccagtt
     5941 gcacgtcatt aggttctgca gcataacaac cgtaattctg tttttggtca gaggttagaa
     6001 agtcgacagg catatcaggc tccctacctg acagttattc attaacaatt ttgcaaccgt
     6061 ccgaaatatt ataaattatc gcacacataa aaacagtgct gttaatgtgt ctattaaatc
     6121 gattttttgt tataacagac actgcttgtc cgatatttga tttaggatac atttttatgc
     6181 gactttttgg ttacgcacgg gtatcaacca gccagcaatc tctcgatatt caggttcggg
     6241 cactcaaaga cgcaggcgtg aaagcaaatc gcatctttac tgacaaggca tcgggcagtt
     6301 caagcgatcg gaaagggctg gacttgctga ggatgaaggt ggaggaaggt gacgtcatct
     6361 tggtgaagaa acttgaccgc cttgggcgcg atactgctga catgatccag ttaataaaag
     6421 agtttgacgc ccaaggtgta tccattcggt ttattgatga cggaatcagt accgatgggg
     6481 agatgggtaa aatggttgtc actattctat ctgcagtggc ccaggcagaa cgacagagaa
     6541 tactagagcg taccaatgaa ggtcgccaag aggcaatggc aaaaggagtt gtttttggta
     6601 gaaaaagaaa aatagataga gatgcagtat taaatatgtg gcaacagggg ttaggtgcct
     6661 cacatatatc aaaaacaatg aatattgctc gttcaacagt atataaagta ataaatgaaa
     6721 gcaactaaca taaaaggttg aatatggaat tgaatattac ttcaaagtct aatccgtttg
     6781 gtgatacgac agcagaaaat gataaaaaga tgttgagtaa tgctttcatt gaaactgctg
     6841 actttaggac tctaattgaa actgatgaca ggaccattgt tgttggtaga cgagggacag
     6901 gtaaaagtgc tttatttatt cagctaaatg agcattggaa gaaagacaaa aaaatattaa
     6961 tcctgagttt ctcaccagac gattctcaaa ttattggttt cagatcaatg ttgaaaccat
     7021 ttacaggatc atttaattta gcaagggcag caacaagact attatggcga tacgctatgc
     7081 taatggagat agcatcatat atatcatctc attacaaatt gtcatctcaa atatcatcag
     7141 aaacattgtt gaatgaacat ttgaaaaaat ggaattctgc acaaggtgac attttaagaa
     7201 agtgtaggct tgtcgcaaag gagtatttag atgaaaataa cccagaagaa tcaattggtg
     7261 atcttcaatt caatttgaat atctcagaaa tagaaaataa tatagtatca cttcttgaac
     7321 gctcagacag gaaagttgtc atattaatgg ataagctaga tgaggcgtat gaaccggata
     7381 atataggaat tggaatcatt gcaggtctag catatgcatc tattgaatta aatcaaaaag
     7441 caaaatgcat tcgtccaata atttttttaa gggataatat atttaggtcg ctatcaaagg
     7501 aagatcctga ttactcgaga aatatagagg gtcaagtcat aaggttgcat tgggactggg
     7561 cacaactcct aatgctgtca gctaaaagaa tgaaagtagc atttaagcta gatattgaga
     7621 aagatcaacg agtttgggat agatgcacag cggatgatct taaagggagg aatggtttta
     7681 agcgatgttt gcaatttacc ctttaccggc ccagggattt actatcattg ttgaatgaag
     7741 cttttttttc cgcattcaga gagaatagag aaactatcat aaacactgac ctagaatatg
     7801 cagccaagtc aatttccatg gccagacttg aagatctctg gaaagagtat cagaagatct
     7861 ttccttcaat acaggttata actagtgcat ttcgtagcat tgaacctgaa ttaacagttt
     7921 atacgtgctt aaaaaaaata gaagcatctt tcgaattaat cgaagaaaat ggagatccta
     7981 aaataacgtc tgaaatacag ttgttaaagg caagtggaat tctccaaagt ttgtatagtg
     8041 taggatttgt tggtattcgt gataaaaaca cctcatccta ttcattttgc catgacgggc
     8101 gaactccaga taaaggtttt gaaagtaatg agaaattatt aattcatcca tgttactggc
     8161 ttggtttgaa tctaaatcgt aatgcacttg ctccagagga agcagaggag ataaatgacg
     8221 aatatgatat taatattatt tcagataatt cagccattag aaataaaaca ataggtcaaa
     8281 taactactca tctagatcag ataccgatag gaaatgaagg tgccactgaa tttgaacaat
     8341 ggtgtttaga cgcactaaga atagtatttg catcccacct aacagacatc aagtcccatc
     8401 caaatggtaa cgcagttcag agacgagata ttataggcac caatggtggc aaatctgatt
     8461 tttggaaacg agtattggag gactataaaa caagacaagt tgtttttgat gctaaaaatt
     8521 ttgaagaatt aggtccatct gagtatagac aattacaatc atatttaaca ggtccgtatg
     8581 gaaagctcgg ttttataatt aatagagatg agagtgaggt attgaaatca ggaaaagatt
     8641 tagattggac aaaggaaatg tatcaatccc ataattcatt aataataaag ttacctgcga
     8701 aatatatatc taaactgctg cagaaattaa gaaatcctga aaaacatgac gctatagata
     8761 gacagatggg gaaactttta actttatatg aaacatcata tatggctatt aaaagcaccc
     8821 aaaagaaaag aagaaaataa aatcagctac aacatacgaa agggtccctg aaaagggacc
     8881 tttgtatact gtattataat caataagtta taccataaac gtacgttttc gttccattgg
     8941 ccctcaaacc ccatgataaa gtgaatcaag agctagaaaa ggatctcaag gacaacattg
     9001 ctctgggacg gaaaaacttg caggactctt taagaaccca ggaagtggtc gcgcaggagc
     9061 agaaagatct gcgtatccgt cagattcagg aagcgttgca gtatgcgaat caggcgcagg
     9121 tgacaaagcc acaggttcag cagactgaag atgtgacgca agatacgttg ttccttctag
     9181 ggagcgaagc gctggagtcg atgattaagc atgaagcgac tcgtccgttg gtgttctcac
     9241 caaactacta tcagacacgt caaaacctgt tggatattga aaaattaaag tttgatgatc
     9301 ttgatattca tgcttaccgc tatgtgatga aaccgacgtt acctattcgt cgcgatagtc
     9361 cgaaaaaggc aatcaccttg attctggcag tgcttctggg cggcatggtt ggcgcgggga
     9421 ttgtgttggg gcgtaacgct ctgcgtaatt acaacgcgaa gtaatattat tgtgcattta
     9481 agagaaacgg gcagggtggt gacaccatgc ccgttttttt tgccggatgc gatgctggcg
     9541 catcttatcc ggcctacgtg tgttgagata atgtgtaggc acgataagtt tgcgcatcgg
     9601 gcaatggctc cgggtgtgac aacaacatca cacctgctcc ccgggtcgac ctgcagccaa
     9661 gcttaattag ctgagcttgg actcctgttg atagatccag taatgacctc agaactccat
     9721 ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaagc
     9781 tagcttggcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
     9841 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     9901 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
     9961 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
    10021 tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
    10081 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
    10141 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
    10201 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
    10261 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
    10321 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
    10381 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
    10441 acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca gttattggtg
    10501 cccttaaacg cctggggtaa tgactctcta gcttgaggca tcaaataaaa cgaaaggctc
    10561 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaac
//
