LOCUS       sequence-340823-       10607 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pQE30 encoding Wzz mutant  i255.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10607)
  AUTHORS   Papadopoulos M, Morona R
  TITLE     Mutagenesis and chemical cross-linking suggest that Wzz dimer 
            stability and oligomerization affect lipopolysaccharide O-antigen 
            modal chain length control.
  JOURNAL   J Bacteriol. 2010 Jul;192(13):3385-93. doi: 10.1128/JB.01134-09. 
            Epub 2010 May 7.
  PUBMED    20453100
REFERENCE   2  (bases 1 to 10607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JB.01134-09"; journalName: "J Bacteriol"; date: "2010-07"; 
            volume: "192"; issue: "13"; pages: "3385-93"
COMMENT     Sequence Label: pMPRMA47
FEATURES             Location/Qualifiers
     source          1..10607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      13..99
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(146..168)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    254..394
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     309..328
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(409..426)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(580..1168)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(660..679)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1339..2130,2131..2199))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1962..1981
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2200..2304)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2372..2390
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(2414..2428,2429..2434,2435..2451,2452..2458)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    2434..2450
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2466..2482
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2501..2512
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2531..2548
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      9758..9852
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             9896..10555
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(9962..9981)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agcttgaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
       61 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccctctag agctgcctcg
      121 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
      181 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
      241 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
      301 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
      361 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
      421 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      481 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      541 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
      601 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      661 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      721 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
      781 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
      841 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
      901 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
      961 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1021 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1081 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1141 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1201 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1261 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1321 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     1381 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     1441 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     1501 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     1561 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     1621 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     1681 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     1741 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     1801 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     1861 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     1921 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     1981 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     2041 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     2101 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     2161 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     2221 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     2281 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     2341 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     2401 tcacctcgag aaatcataaa aaatttattt gctttgtgag cggataacaa ttataataga
     2461 ttcaattgtg agcggataac aatttcacac agaattcatt aaagaggaga aattaactat
     2521 gagaggatcg catcaccatc accatcacgg atccgcatgc gagctcagag tagaaaataa
     2581 taatgtttct gggcaaaacc atgacccgga acagattgat ttgattgatt tactagtgca
     2641 gttgtggcgt ggcaagatga caattatcat ttccgtcatt gtggctattg ccctggctat
     2701 tggttatttg gcagtagcga aggagaaatg gacgtcaaca gcaattatca ctcagcccga
     2761 cgtggggcaa attgctggct ataacaatgc catgaatgtt atctatggtc aggctgcacc
     2821 gaaagtatcg gatttgcagg agacgttaat tggtcgcttc agttctgcct tctctgcatt
     2881 agcagaaacg ctggataatc aggaagagcc agaaaaactt accatcgaac cttctgttaa
     2941 gaaccagcaa ttaccattga ctgtttctta tgttgggcaa actgcagagg gcgcacaaat
     3001 gaagttggcc caatacattc agcaagttga tgataaaggg ggtttgaggg ccaatggaac
     3061 gaaaacgtac gttaaggaga taattcgttg tttatattta aatttagagc tctcagttcc
     3121 ccttttaaaa tatcctctgg caacgtgaat gtataatggc ccaacatatt gatatgcccg
     3181 tgcatcaggg gagatagccg agcgatatct tcatctataa tttcttcgcc attacggcgc
     3241 atccagctca acgcttcctc catatagagc gtgttccaca gaaccactgc attagtaacc
     3301 aggcccagcg cccccagttg atcttcctgc ccttcacgat aacgctttct gatctctccg
     3361 cgttgtccgt aacaaatcgc acgagccaca gcgtggcggc cttctcctcg attaagctgc
     3421 gtcaggatcc gccgacgata atcttcatca tcaatataat tgaggagata tagcgttttg
     3481 tttacacgcc ctacttccat aattgcctgt gccagtcctg atgggcgcga gcttttcagt
     3541 aaagagcgaa tgagttctga cgcatgaatt gtacccaact tcagcgaacc agcggttcgc
     3601 atcatctcat cccactgact ctccgctttt gacagatctg catatcctcg ggccaactta
     3661 tccagtactc cgtagtttgc cgatttattc acccgccaga acaccgcctc acctgcatcg
     3721 gcaagccggg gggaaaactg ataccccagt agccagaaca gaccgaaaat aatatcgctg
     3781 ctacccgcag tgtctgtcat gatttcaact ggattcagcc ctgtctgctg ctcaagaagt
     3841 ccttccagta caaaaatcga atcccgtaat gtaccgggta ccacaatgcc atggaaccca
     3901 gagtactgat cagatacgaa gttataccag gtgatgcctc gtccagaacc aaaatatttt
     3961 ctgttagatc ctgagttgat ggtcttcact ggtgtgacaa agcgcatgcc gtcagctgat
     4021 gccacttctc cacctcccca acgaccagca agctccagtg tggactgaaa atcaaccagg
     4081 cgggcattgg cgctgaccag cgtttctgca cgaaggtaat tctgtttcac ccaactgagc
     4141 cgatggcggg tcagtgctgg tatattgtgc tttatcagcg gttccagtcc gatattacag
     4201 gcttcagcca tcaataccgc acataaactg atgtgcaaat cttgcgctcg agcaccagat
     4261 tcactgacat gcgcaaactc atgtgtaaat cctgtctggg catctatctc aagtaacagt
     4321 tccgttaaat ctaccggtgg gagtagctgt ttgatccgat tatttagacg aagcaatgat
     4381 ggtggctctt cctgtttctc cagacaactg atagtcaggg atggatattt accttcatta
     4441 cagatatgaa cttccgcatt cttttcaaat cgtgatgcca cggctttcca ggtctcatcc
     4501 agctgaatag ccagttgttg cacaccttta cgtccatcga caggatgtcc cagtgcccga
     4561 cagacaggaa tacgctgagt ctgccactct tcaccttgca acaacttctc gcgaggatct
     4621 ccccagcgat cactgttttc aagccagatg tcccggcggc gcagtgcatc ctgaaggcgt
     4681 tccagcaaac atagtgaata acctgcacgc tgtatccgtc cctccgcatc gtatacgagg
     4741 cgtttccagg gaccggtgat aatatgttca ggcgcatcat caaggatgcg ctttttcgaa
     4801 ccattcagtt ctgccagata atgaatcgca gccagtacat gttcacctgc cggtgccgca
     4861 cggaaatgca ggtcccgcaa caccgccgga agaaaacgtt taacccgacc gtactgctca
     4921 accatttcgt catggaaatt attgttctgt ggacgagcaa gttcattaac cttgcttaca
     4981 gattctgcca gtctgttttt gggtacgcac ttgaagataa cctgcctgag atctgggaca
     5041 tctgtattat catccagcaa caatgcacat gcccgcgcca gtaacaatgc ggcctgatca
     5101 agatctttca gtgtcctgag tctttttttt tgcccggttt tctttgcttc gcggataatg
     5161 tccagaatta gcatatcaag cacatcaacg gcatcgtcta atgccgttat ttcctgtgct
     5221 ttaacgaatg cagtaagtac agcaagcttt ctctgctgtg gcattcgagc gatatatttt
     5281 accgacgcca tgccagcata acgagccaga ttacgcagtt gaatggcagg cagaccggaa
     5341 aagttcagtc gggaaaactc aagacttcgt aatcggatat atcgttccag cgcttcatta
     5401 aacgcaggtc cactgactgt aacagggcct tttttcaact gttccagtgc tgatacacgc
     5461 tgaccttccg gaatgaccag aagctccatc acttgagctg cctgccattt gttcggcagt
     5521 gcggccagct ttttccacag ccgctgattt gccctttcac gaatttcact gatgagacgt
     5581 actagtgtgg ttgctccggg cagtaatacc ttattttgaa gcaaccatgc agtggcaaaa
     5641 tcaaacatca gacccggtcg ctcattactg agccacgccc gggtatatag cagacgcttc
     5701 aggcggaaag accatggaaa atcaccaaat tcatgatagc cgtaatattc cttaattaat
     5761 gcagtatgtt ctctaagggt agtgtcccgt tcagcatagc gggagagaac ttctggacgg
     5821 tggatattaa gctgtaccgc gacaaaatgt tgaacaccag gcagaacctg agttaaatcc
     5881 gtcagaaatg ttcccagaaa acgggctgtg gtgagctgaa gcgcaatgcc cagcctatta
     5941 tgtttgcccc gtcgttggtt aatgaaggcc agatcccgtt catcaagatg aaaatagcgc
     6001 gccagttgca cgtcattagg ttctgcagca taacaaccgt aattctgttt ttggtcagag
     6061 gttagaaagt cgacaggcat atcaggctcc ctacctgaca gttattcatt aacaattttg
     6121 caaccgtccg aaatattata aattatcgca cacataaaaa cagtgctgtt aatgtgtcta
     6181 ttaaatcgat tttttgttat aacagacact gcttgtccga tatttgattt aggatacatt
     6241 tttatgcgac tttttggtta cgcacgggta tcaaccagcc agcaatctct cgatattcag
     6301 gttcgggcac tcaaagacgc aggcgtgaaa gcaaatcgca tctttactga caaggcatcg
     6361 ggcagttcaa gcgatcggaa agggctggac ttgctgagga tgaaggtgga ggaaggtgac
     6421 gtcatcttgg tgaagaaact tgaccgcctt gggcgcgata ctgctgacat gatccagtta
     6481 ataaaagagt ttgacgccca aggtgtatcc attcggttta ttgatgacgg aatcagtacc
     6541 gatggggaga tgggtaaaat ggttgtcact attctatctg cagtggccca ggcagaacga
     6601 cagagaatac tagagcgtac caatgaaggt cgccaagagg caatggcaaa aggagttgtt
     6661 tttggtagaa aaagaaaaat agatagagat gcagtattaa atatgtggca acaggggtta
     6721 ggtgcctcac atatatcaaa aacaatgaat attgctcgtt caacagtata taaagtaata
     6781 aatgaaagca actaacataa aaggttgaat atggaattga atattacttc aaagtctaat
     6841 ccgtttggtg atacgacagc agaaaatgat aaaaagatgt tgagtaatgc tttcattgaa
     6901 actgctgact ttaggactct aattgaaact gatgacagga ccattgttgt tggtagacga
     6961 gggacaggta aaagtgcttt atttattcag ctaaatgagc attggaagaa agacaaaaaa
     7021 atattaatcc tgagtttctc accagacgat tctcaaatta ttggtttcag atcaatgttg
     7081 aaaccattta caggatcatt taatttagca agggcagcaa caagactatt atggcgatac
     7141 gctatgctaa tggagatagc atcatatata tcatctcatt acaaattgtc atctcaaata
     7201 tcatcagaaa cattgttgaa tgaacatttg aaaaaatgga attctgcaca aggtgacatt
     7261 ttaagaaagt gtaggcttgt cgcaaaggag tatttagatg aaaataaccc agaagaatca
     7321 attggtgatc ttcaattcaa tttgaatatc tcagaaatag aaaataatat agtatcactt
     7381 cttgaacgct cagacaggaa agttgtcata ttaatggata agctagatga ggcgtatgaa
     7441 ccggataata taggaattgg aatcattgca ggtctagcat atgcatctat tgaattaaat
     7501 caaaaagcaa aatgcattcg tccaataatt tttttaaggg ataatatatt taggtcgcta
     7561 tcaaaggaag atcctgatta ctcgagaaat atagagggtc aagtcataag gttgcattgg
     7621 gactgggcac aactcctaat gctgtcagct aaaagaatga aagtagcatt taagctagat
     7681 attgagaaag atcaacgagt ttgggataga tgcacagcgg atgatcttaa agggaggaat
     7741 ggttttaagc gatgtttgca atttaccctt taccggccca gggatttact atcattgttg
     7801 aatgaagctt ttttttccgc attcagagag aatagagaaa ctatcataaa cactgaccta
     7861 gaatatgcag ccaagtcaat ttccatggcc agacttgaag atctctggaa agagtatcag
     7921 aagatctttc cttcaataca ggttataact agtgcatttc gtagcattga acctgaatta
     7981 acagtttata cgtgcttaaa aaaaatagaa gcatctttcg aattaatcga agaaaatgga
     8041 gatcctaaaa taacgtctga aatacagttg ttaaaggcaa gtggaattct ccaaagtttg
     8101 tatagtgtag gatttgttgg tattcgtgat aaaaacacct catcctattc attttgccat
     8161 gacgggcgaa ctccagataa aggttttgaa agtaatgaga aattattaat tcatccatgt
     8221 tactggcttg gtttgaatct aaatcgtaat gcacttgctc cagaggaagc agaggagata
     8281 aatgacgaat atgatattaa tattatttca gataattcag ccattagaaa taaaacaata
     8341 ggtcaaataa ctactcatct agatcagata ccgataggaa atgaaggtgc cactgaattt
     8401 gaacaatggt gtttagacgc actaagaata gtatttgcat cccacctaac agacatcaag
     8461 tcccatccaa atggtaacgc agttcagaga cgagatatta taggcaccaa tggtggcaaa
     8521 tctgattttt ggaaacgagt attggaggac tataaaacaa gacaagttgt ttttgatgct
     8581 aaaaattttg aagaattagg tccatctgag tatagacaat tacaatcata tttaacaggt
     8641 ccgtatggaa agctcggttt tataattaat agagatgaga gtgaggtatt gaaatcagga
     8701 aaagatttag attggacaaa ggaaatgtat caatcccata attcattaat aataaagtta
     8761 cctgcgaaat atatatctaa actgctgcag aaattaagaa atcctgaaaa acatgacgct
     8821 atagatagac agatggggaa acttttaact ttatatgaaa catcatatat ggctattaaa
     8881 agcacccaaa agaaaagaag aaaataaaat cagctacaac atacgaaagg gtccctgaaa
     8941 agggaccttt gtatactgta ttataatcaa taagttatac cataaacgta cgttttcgtt
     9001 ccattggccc tcaaacccct aaagtgaatc aagagctaga aaaggatctc aaggacaaca
     9061 ttgctctggg acggaaaaac ttgcaggact ctttaagaac ccaggaagtg gtcgcgcagg
     9121 agcagaaaga tctgcgtatc cgtcagattc aggaagcgtt gcagtatgcg aatcaggcgc
     9181 aggtgacaaa gccacaggtt cagcagactg aagatgtgac gcaagatacg ttgttccttc
     9241 tagggagcga agcgctggag tcgatgatta agcatgaagc gactcgtccg ttggtgttct
     9301 caccaaacta ctattgcggc cgcaactatc agacacgtca aaacctgttg gatattgaaa
     9361 aattaaagtt tgatgatctt gatattcatg cttaccgcta tgtgatgaaa ccgacgttac
     9421 ctattcgtcg cgatagtccg aaaaaggcaa tcaccttgat tctggcagtg cttctgggcg
     9481 gcatggttgg cgcggggatt gtgttggggc gtaacgctct gcgtaattac aacgcgaagt
     9541 aatattattg tgcatttaag agaaacgggc agggtggtga caccatgccc gttttttttg
     9601 ccggatgcga tgctggcgca tcttatccgg cctacgtgtg ttgagataat gtgtaggcac
     9661 gataagtttg cgcatcgggc aatggctccg ggtgtgacaa caacatcaca cctgctcccc
     9721 gggtcgacct gcagccaagc ttaattagct gagcttggac tcctgttgat agatccagta
     9781 atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt
     9841 attggtgaga atccaagcta gcttggcgag attttcagga gctaaggaag ctaaaatgga
     9901 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
     9961 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
    10021 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
    10081 tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct
    10141 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
    10201 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
    10261 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
    10321 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
    10381 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa
    10441 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg
    10501 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt
    10561 ttaaggcagt tattggtgcc cttaaacgcc tggggtaatg actctct
//
