LOCUS       sequence-340918-        7731 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7731)
  AUTHORS   Aihara S, Fujimoto S, Sakaguchi R, Imai T
  TITLE     BMPR-2 gates activity-dependent stabilization of primary dendrites 
            during mitral cell remodeling.
  JOURNAL   Cell Rep. 2021 Jun 22;35(12):109276. doi: 
            10.1016/j.celrep.2021.109276.
  PUBMED    34161760
REFERENCE   2  (bases 1 to 7731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-06-22"; volume: "35(12):109276. doi"; pages: " 
            10.1016/j.celrep.2021.109276"
COMMENT     Sequence Label: pCAG-Bmpr2-Delta-751-813aa
FEATURES             Location/Qualifiers
     source          1..7731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..662
                     /label=chicken beta-actin promoter
     intron          663..1679
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1603..1625
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1687..1706
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(4753..4772)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4818..4873
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4872..4891)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5234..5250)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5234..5250)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5247..5269)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5258..5274
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5282..5288,5289..5306,5307..5312))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5327..5348
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5407..5602
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5453..5588
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5608..5742
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5645..5664)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5699..5718
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5810..5827)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5981..6569)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6061..6080)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6740..7531,7532..7600))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7363..7382
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7601..7705)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggnsgcg cgccrggsgg ggsggggsgg ggsgrggggs
      541 ggggsggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcgccacca
     1741 tgacttcctc gctgcatcgg ccctttcggg tgccctggct gctatgggcc gtcctgctgg
     1801 tcagcactac ggctgcttct cagaatcaag aacggctgtg tgcatttaaa gatccatatc
     1861 aacaagatct tgggataggt gagagtcgaa tctctcatga aaatgggaca atattatgtt
     1921 ccaaagggag cacgtgttat ggtctgtggg agaaatcaaa aggggacatc aatcttgtga
     1981 aacaaggatg ttggtctcac atcggtgatc cccaagagtg ccactatgaa gagtgtgtag
     2041 taactaccac cccaccctca attcagaatg gaacgtaccg cttttgctgc tgtagtacag
     2101 atttatgtaa tgtcaacttt actgagaact ttccaccccc tgacacaaca ccactcagtc
     2161 cacctcattc atttaatcga gatgaaacga taatcattgc tttggcatca gtttctgtgt
     2221 tagctgtttt gatagtcgcc ttatgttttg gatacagaat gttgacagga gaccggaaac
     2281 agggtcttca cagcatgaac atgatggagg cggcagcagc agagccctcc cttgacctgg
     2341 ataacctgaa gctgctggag ctgattggac ggggtcgata cggagcagta tataaaggtt
     2401 ccttggatga gcgtccagtt gctgtaaaag tattttcttt tgcaaaccgt cagaatttta
     2461 taaatgaaaa aaacatttac agagtgcctt tgatggaaca tgacaacatt gctcgcttca
     2521 tagttggaga cgagaggctc actgcagacg gccgcatgga gtatttgctt gtgatggagt
     2581 attatcccaa tggatctctg tgcaaatatc tgagtctcca cacaagtgat tgggtaagct
     2641 cttgccgtct ggctcattct gtgactagag gactggctta tcttcacaca gaattaccac
     2701 gaggagatca ttataaaccc gcaatctccc accgagattt aaacagcagg aatgtcctgg
     2761 taaagaatga cggcgcgtgt gttatcagtg actttggttt atccatgagg ctaactggaa
     2821 atcggctggt gcgcccaggg gaagaagata atgcggctat aagtgaggtt ggcacaattc
     2881 gctatatggc accagaagtg ctagaaggag ctgtgaacct gagggactgt gagtcagctc
     2941 tgaagcaagt ggacatgtat gcgcttggac tcatctactg ggaggtgttt atgaggtgta
     3001 cagacctctt cccaggtgaa tctgtaccag attaccagat ggcttttcag acagaagttg
     3061 gaaaccatcc cacatttgag gatatgcagg ttcttgtgtc cagagagaag cagagaccca
     3121 agttcccaga agcctggaaa gaaaatagcc tggcagtgag gtcactcaag gaaacaattg
     3181 aagactgctg ggaccaggat gcagaggctc ggctcactgc acagtgtgct gaggagagga
     3241 tggctgaact catgatgata tgggagagaa acaagtctgt gagcccaacg gtcaacccaa
     3301 tgtcaactgc tatgcagaat gaacgcaacc tgtcacataa taggcgtgtg ccaaaaatcg
     3361 ggccttaccc agattattcc tcttcctcat atattgaaga ctctatccat catactgaca
     3421 gcattgtgaa gaatatttcc tctgagcatt cgatgtccag cacaccattg acaataggag
     3481 aaaagaatcg aaattcaatt aattatgaac gacagcaagc acaagctcga atccctagcc
     3541 cagaaacaag cgtcacaagc ctgtccacaa acacaaccac cacaaacacc accggcctca
     3601 ctccaagtac tggcatgacc actatatctg agatgccata cccagatgag acacatttgc
     3661 acgccacaaa tgttgcacag tcaatcgggc caacccctgt ctgcttacag ctgacagaag
     3721 aagacttgga gactaataag ctagatccaa aagaagttga taagaacctc aaggaaagct
     3781 ctgatgagaa tctcatggag cattctctga agcagttcag tgggccagac ccattgagca
     3841 gtaccagttc tagcttgctt tatccactca taaagctcgc agtggaagtg actggacaac
     3901 aggacttcac acaggctgca aatgggcaag catgtttaat tcctgatgtt ccacctgctc
     3961 agatctatcc tctccctaag caacagaacc acaatgttaa ttcccatgct gccacaaccc
     4021 agtatgccaa tggcgcagtg ccagctggcc aggcagccaa catagtggca cataggtccc
     4081 aagaaatgct gcagaatcaa tttattggtg aggataccag gctgaatatc aattccagtc
     4141 ctgatgagca tgaaccttta ctgagacgag agcaacaggc tggccatgat gaaggggttc
     4201 tggatcgttt ggtagatagg agggaacggc cattagaagg tggccgaaca aattccaata
     4261 acaacaacag caatccatgt tcagaacaag atatccttac acaaggtgtt acaagcacag
     4321 ctgcagatcc tgggccatca aagcccagaa gagcacagag gcccaattct ctggatcttt
     4381 cagccacaaa tatcctggat ggcagcagta tacagatagg tgagtcaaca caagatggca
     4441 aatcaggatc aggtgaaaag atcaagagac gtgtgaaaac tccatactct cttaagcggt
     4501 ggcgcccgtc cacctgggtc atctccaccg agccgctgga ctgtgaggtc aacaacaatg
     4561 gcagtgacag ggcagtccat tctaaatcta gcactgctgt gtaccttgca gagggaggca
     4621 ctgccacgac cacagtgtct aaagatatag gaatgaattg tctgtgagcg gccgcactcc
     4681 tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa
     4741 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
     4801 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     4861 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
     4921 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg
     4981 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag
     5041 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt
     5101 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact
     5161 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct
     5221 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     5281 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     5341 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     5401 ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     5461 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     5521 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     5581 tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa
     5641 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5701 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg
     5761 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     5821 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     5881 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     5941 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6001 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6061 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6121 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6181 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6241 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6301 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6361 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6421 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6481 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6541 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6601 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6661 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6721 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6781 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6841 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6901 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6961 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7021 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7081 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7141 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7201 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7261 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7321 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7381 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7441 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7501 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7561 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7621 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7681 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct g
//
