LOCUS       sequence-340925-        6095 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses BMP-7 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Aihara S, Fujimoto S, Sakaguchi R, Imai T
  TITLE     BMPR-2 gates activity-dependent stabilization of primary dendrites 
            during mitral cell remodeling.
  JOURNAL   Cell Rep. 2021 Jun 22;35(12):109276. doi: 
            10.1016/j.celrep.2021.109276.
  PUBMED    34161760
REFERENCE   2  (bases 1 to 6095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-06-22"; volume: "35(12):109276. doi"; pages: " 
            10.1016/j.celrep.2021.109276"
COMMENT     Sequence Label: pCAG-Bmp7
FEATURES             Location/Qualifiers
     source          1..6095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(3117..3136)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3182..3237
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3236..3255)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3598..3614)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3598..3614)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3611..3633)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3622..3638
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3646..3652,3653..3670,3671..3676))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3691..3712
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3771..3966
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3817..3952
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3972..4106
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4009..4028)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4063..4082
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4174..4191)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4345..4933)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4425..4444)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5104..5895,5896..5964))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5965..6069)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggsgcgc gccrggsggg gsggggsggg gsgrggggsg
      541 gggsggggsg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgccaccat
     1741 gcacgtgcgc tcgctgcgcg ctgcggcgcc acacagcttc gtggcgctct gggcgcctct
     1801 gttcttgctg cgctccgccc tggccgattt cagcctggac aacgaggtgc actccagctt
     1861 catccaccgg cgcctccgca gccaggagcg gcgggagatg cagcgggaga tcctgtccat
     1921 cttagggttg ccccatcgcc cgcgcccgca cctccaggga aagcataatt cggcgcccat
     1981 gttcatgttg gacctgtaca acgccatggc ggtggaggag agcgggccgg acggacaggg
     2041 cttctcctac ccctacaagg ccgtcttcag tacccagggc ccccctttag ccagcctgca
     2101 ggacagccac ttcctcactg acgccgacat ggtcatgagc ttcgtcaacc tagtggaaca
     2161 tgacaaagaa ttcttccacc ctcgatacca ccatcgggag ttccggtttg atctttccaa
     2221 gatccccgag ggcgaagcgg tgaccgcagc cgaattcagg atctataagg actacatccg
     2281 ggagcgattt gacaacgaga ccttccagat cacagtctat caggtgctcc aggagcactc
     2341 aggcagggag tcggacctct tcttgctgga cagccgcacc atctgggctt ctgaggaggg
     2401 ctggttggtg tttgatatca cagccaccag caaccactgg gtggtcaacc ctcggcacaa
     2461 cctgggctta cagctctctg tggagaccct ggatgggcag agcatcaacc ccaagttggc
     2521 aggcctgatt ggacggcatg gaccccagaa caagcaaccc ttcatggtgg ccttcttcaa
     2581 ggccacggaa gtccatctcc gtagtatccg gtccacgggg ggcaagcagc gcagccagaa
     2641 tcgctccaag acgccaaaga accaagaggc cctgaggatg gccagtgtgg cagaaaacag
     2701 cagcagtgac cagaggcagg cctgcaagaa acatgagctg tacgtcagct tccgagacct
     2761 tggctggcag gactggatca ttgcacctga aggctatgct gcctactact gtgagggaga
     2821 gtgcgccttc cctctgaact cctacatgaa cgccaccaac cacgccatcg tccagacact
     2881 ggttcacttc atcaacccag acacagtacc caagccctgc tgtgcgccca cccagctcaa
     2941 cgccatctct gtcctctact tcgacgacag ctctaatgtc atcctgaaga agtacagaaa
     3001 catggtggtc cgggcctgtg gctgccacta ggcggccgca ctcctcaggt gcaggctgcc
     3061 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt
     3121 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
     3181 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
     3241 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
     3301 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
     3361 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     3421 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     3481 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
     3541 gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc
     3601 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     3661 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     3721 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat
     3781 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     3841 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     3901 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     3961 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4021 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4081 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag
     4141 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     4201 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     4261 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     4321 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     4381 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     4441 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4501 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4561 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4621 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4681 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4741 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     4801 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4861 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4921 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     4981 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5041 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     5101 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     5161 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     5221 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     5281 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     5341 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     5401 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     5461 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     5521 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     5581 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     5641 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     5701 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     5761 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     5821 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     5881 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     5941 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     6001 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     6061 ggttccgcgc acatttcccc gaaaagtgcc acctg
//
