LOCUS       sequence-340974-       12301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C-terminal flag-tagged CAD with deletion of amino acids 
            F1366 - P1376 in allosteric domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12301)
  AUTHORS   Shin J, Mir H, Khurram MA, Fujihara KM, Dynlacht BD, Cardozo TJ, 
            Possemato R
  TITLE     Allosteric regulation of CAD modulates de novo pyrimidine synthesis 
            during the cell cycle.
  JOURNAL   Nat Metab. 2023 Feb 6. doi: 10.1038/s42255-023-00735-9.
  PUBMED    36747088
REFERENCE   2  (bases 1 to 12301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Metab. 
            2023 Feb 6. doi: 10.1038/s42255-023-00735-9."
COMMENT     Sequence Label: pMXS-IRES-BLAST flag-CAD delta Loop1
FEATURES             Location/Qualifiers
     source          1..12301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     7..29
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     45..61
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    87..461
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     399..415
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      488..497
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7132..7155
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(7220..7794,7795..7797,7798..7806)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7387..7404)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7614..7633
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7807..8202
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             8373..8842
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8972..8989)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9143..9731)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9223..9242)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9895..10686,10687..10755))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10518..10537
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10756..10860)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             10890..11483
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    11546..11903
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             join(11962..12301,1..77)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
ORIGIN
        1 ctctccccct tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca
       61 ctccttctct aggcgccccc atatggccat atgagatctt atatggggca cccccgcccc
      121 ttgtaaactt ccctgaccct gacatgacaa gagttactaa cagcccctct ctccaagctc
      181 acttacaggc tctctactta gtccagcacg aagtctggag acctctggcg gcagcctacc
      241 aagaacaact ggaccgaccg gtggtacctc acccttaccg agtcggcgac acagtgtggg
      301 tccgccgaca ccagactaag aacctagaac ctcgctggaa aggaccttac acagtcctgc
      361 tgaccacccc caccgccctc aaagtagacg gcatcgcagc ttggatacac gccgcccacg
      421 tgaaggctgc cgaccccggg ggtggaccat cctctagact gccggatcta gctagttaat
      481 taaggatcca tggcggccct agtgttggag gacgggtcgg tcctgcgggg ccagcccttt
      541 ggggccgccg tgtcgactgc cggggaagtg gtgtttcaaa ccggcatggt cggctacccc
      601 gaggccctca ctgatccctc ctacaaggca cagatcttag tgctcaccta tcctctgatc
      661 ggcaactatg gcatcccccc agatgaaatg gatgagttcg gtctctgcaa gtggtttgaa
      721 tcctcgggca tccacgtagc agcactggta gtgggagagt gctgtcctac tcccagccac
      781 tggagtgcca cccgcaccct gcatgagtgg ctgcagcagc atggcatccc tggcttgcaa
      841 ggagtagaca ctcgggagct gaccaagaag ttgcgggaac aggggtctct gctggggaag
      901 ctggtccaga atggaacaga accttcaagt ctgccattct tggaccccaa tgcccgcccc
      961 ctggtaccag aggtctccat taagactcca cgggtattca atacaggggg tgcccctcgg
     1021 atccttgctt tggactgtgg cctcaagtat aatcagatcc gatgcctctg ccagcgtggg
     1081 gctgaggtca ctgtggtacc ctgggaccat gcactagaca gccaagagta tgagggtctc
     1141 ttcttaagta atgggcctgg tgaccctgcc tcctatccca gtgtcgtatc cacactgagc
     1201 cgtgttttat ctgagcctaa tccccgacct gtctttggga tctgcctggg acaccagcta
     1261 ttggccttag ccattggggc caagacttac aagatgagat atgggaaccg aggccataac
     1321 cagccctgct tgttggtggg ctctgggcgc tgctttctga catcccagaa ccatgggttt
     1381 gctgtggaga cagactcact gccagcagac tgggctcctc tcttcaccaa cgccaatgat
     1441 ggttccaatg aaggcattgt gcacaacagc ttgcctttct tcagtgtcca gtttcaccca
     1501 gagcaccaag ctggcccttc agatatggaa ctgcttttcg atatctttct ggaaactgtg
     1561 aaagaggcca cagctgggaa ccctgggggc cagacagtta gagagcggct gactgagcgc
     1621 ctctgtcccc ctgggattcc cactcccggc tctggacttc caccaccacg aaaggttctg
     1681 atcctgggct cagggggcct ctccattggc caagctggag aatttgacta ctcgggctct
     1741 caggcaatta aggccctgaa ggaggaaaac atccagacgt tgctgatcaa ccccaatatt
     1801 gccacagtgc agacctccca ggggctggcc gacaaggtct attttcttcc cataacacct
     1861 cattatgtaa cccaggtgat acgtaatgaa cgccccgatg gtgtgttact gacttttggg
     1921 ggccagactg ctctgaactg tggtgtggag ctgaccaagg ccggggtgct ggctcggtat
     1981 ggggtccggg tcctgggcac accagtggag accattgagc tgaccgagga tcgacgggcc
     2041 tttgctgcca gaatggcaga gatcggagag catgtggccc cgagcgaggc agcaaattct
     2101 cttgaacagg cccaggcagc cgctgaacgg ctggggtacc ctgtgctagt gcgtgcagcc
     2161 tttgccctgg gtggcctggg ctctggcttt gcctctaaca gggaggagct ctctgctctc
     2221 gtggccccag cttttgccca taccagccaa gtgctagtag acaagtctct gaagggatgg
     2281 aaggagattg agtacgaggt ggtgagagac gcctatggca actgtgtcac ggtgtgtaac
     2341 atggagaact tggacccact gggcatccac actggtgagt ccatagtggt ggcccctagc
     2401 cagacactga atgacaggga gtatcagctc ctgaggcaga cagctatcaa ggtgacccag
     2461 cacctgggaa ttgttgggga gtgcaatgtg cagtatgcct tgaaccctga gtctgagcag
     2521 tattacatca ttgaagtgaa tgccaggctc tctcgcagct ctgccctggc cagtaaggcc
     2581 acaggttatc cactggctta tgtggcagcc aagctagcat tgggcatccc tttgcctgag
     2641 ctcaggaact ctgtgacagg gggtacagca gcctttgaac ccagcgtgga ttattgtgtg
     2701 gtgaagattc ctcgatggga ccttagcaag ttcctgcgag tcagcacaaa gattgggagc
     2761 tgcatgaaga gcgttggtga agtcatgggc attgggcgtt catttgagga ggccttccag
     2821 aaggccctgc gcatggtgga tgagaactgt gtgggctttg atcacacagt gaaaccagtc
     2881 agcgatatgg agttggagac tccaacagat aagcggattt ttgtggtggc agctgctttg
     2941 tgggctggtt attcagtgga ccgcctgtat gagctcacac gcatcgaccg ctggttcctg
     3001 caccgaatga agcgtatcat cgcacatgcc cagctgctag aacaacaccg tggacagcct
     3061 ttgccgccag acctgctgca acaggccaag tgtcttggct tctcagacaa acagattgcc
     3121 cttgcagttc tgagcacaga gctggctgtt cgcaagctgc gtcaggaact ggggatctgt
     3181 ccagcagtga aacagattga cacagttgca gctgagtggc cagcccagac aaattaccta
     3241 tacctaacgt attggggcac cacccatgac ctcacctttc gaacacctca tgtcctagtc
     3301 cttggctctg gcgtctaccg tattggctct agcgttgaat ttgactggtg tgctgtaggc
     3361 tgcatccagc agctccgaaa gatgggatat aagaccatca tggtgaacta taacccagag
     3421 acagtcagca ccgactatga catgtgtgat cgactctact ttgatgagat ctcttttgag
     3481 gtggtgatgg acatctatga gctcgagaac cctgaaggtg tgatcctatc catgggtgga
     3541 cagctgccca acaacatggc catggcgttg catcggcagc agtgccgggt gctgggcacc
     3601 tcccctgaag ccattgactc ggctgagaac cgtttcaagt tttcccggct ccttgacacc
     3661 attggtatca gccagcctca gtggagggag ctcagtgacc tcgagtctgc tcgccaattc
     3721 tgccagaccg tggggtaccc ctgtgtggtg cgcccctcct atgtgctgag cggtgctgct
     3781 atgaatgtgg cctacacgga tggagacctg gagcgcttcc tgagcagcgc agcagccgtc
     3841 tccaaagagc atcccgtggt catctccaag ttcatccagg aggctaagga gattgacgtg
     3901 gatgccgtgg cctctgatgg tgtggtggca gccatcgcca tctctgagca tgtggagaat
     3961 gcaggtgtgc attcaggtga tgcgacgctg gtgacccccc cacaagatat cactgccaaa
     4021 accctggagc ggatcaaagc cattgtgcat gctgtgggcc aggagctaca ggtcacagga
     4081 cccttcaatc tgcagctcat tgccaaggat gaccagctga aagttattga atgcaacgta
     4141 cgtgtctctc gctccttccc cttcgtttcc aagacactgg gtgtggacct agtagccttg
     4201 gccacgcggg tcatcatggg ggaagaagtg gaacctgtgg ggctaatgac tggttctgga
     4261 gttgtgggag taaaggtgcc tcagttctcc ttctcccgct tggcgggtgc tgacgtggtg
     4321 ttgggtgtgg aaatgaccag tactggggag gtggccggct ttggggagag ccgctgtgag
     4381 gcatacctca aggccatgct aagcactggc tttaagatcc ccaagaagaa tatcctgctg
     4441 accattggca gctataagaa caaaagcgag ctgctcccaa ctgtgcggct actggagagc
     4501 ctgggctaca gcctctatgc cagtctcggc acagctgact tctacactga gcatggcgtc
     4561 aaggtaacag ctgtggactg gcaccagcgg agcatcctgg agcagctagc tgagaaaaac
     4621 tttgagctgg tgattaacct gtcaatgcgt ggagctgggg gccggcgtct ctcttccttt
     4681 gtcaccaagg gctaccgcac ccgacgcttg gccgctgact tctccgtgcc cctaatcatc
     4741 gatatcaagt gcaccaaact ctttgtggag gccctaggcc agatcgggcc agcccctcct
     4801 ttgaaggtgc atgttgactg tatgacctcc caaaagcttg tgcgactgcc gggattgatt
     4861 gatgtccatg tgcacctgcg ggaaccaggt gggacacata aggaggactt tgcttcaggc
     4921 acagccgctg ccctggctgg gggtatcacc atggtgtgtg ccatgcctaa tacccggccc
     4981 cccatcattg acgcccctgc tctggccctg gcccagaagc tggcagaggc tggcgcccgg
     5041 tgcgactttg cgctattcct tggggcctcg tctgaaaatg caggaacctt gggcaccgtg
     5101 gccgggtctg cagccgggct gaagctttac ctcaatgaga ccttctctga gctgcggctg
     5161 gacagcgtgg tccagtggat ggagcatttc gagacatggc cctcccacct ccccattgtg
     5221 gctcacgcgg agcagcaaac cgtggctgct gtcctcatgg tggctcagct cactcagcgc
     5281 tcagtgcaca tatgtcacgt ggcacggaag gaggagatcc tgctaattaa agctgcaaag
     5341 gcacggggct tgccagtgac ctgcgaggtg gctccccacc acctgttcct aagccatgat
     5401 gacctggagc gcctggggcc tgggaagggg gaggtccggc ctgagcttgg ctcccgccag
     5461 gatgtggaag ccctgtggga gaacatggct gtcatcgact gctttgcctc agaccatgct
     5521 ccccatacct tggaggagaa gtgtgggtcc aggcccccac ctgggttccc agggttagag
     5581 accatgctgc cactactcct gacggctgta agcgagggcc ggctcagcct ggacgacctg
     5641 ctgcagcgat tgcaccacaa tcctcggcgc atctttcacc tgcctccgca ggaggacacc
     5701 tatgtggagg tggatctgga gcatgagtgg acaattccca gccacatgcc cttctccaag
     5761 gcccactgga caccttttga agggcagaaa gtgaagggca ccgtccgccg tgtggtcctg
     5821 cgaggggagg ttgcctatat cgatgggcag gttctggtac ccccgggcta tggacaggat
     5881 gtacggaagt ggccacaggg ggctgttcct cagctcccac cctcagcccc tgccactagt
     5941 gagatgacca cgacacctga aagaccccgc cgtggcatcc cagggcttcc tgatggccgc
     6001 ttccatctgc cgccccgaat ccatcgagcc tccgacccag gtttgccagc tgaggagcca
     6061 aaggagaagt cctctcggaa ggtagccgag ccagagctga tgggaacccc tgatggcacc
     6121 tgctaccctc caccaccagt accgagacag gcatctcccc agaacctggg gacccctggc
     6181 ttgctgcacc cccagacctc acccctgctg cactcattag tgggccaaca tatcctgtcc
     6241 gtccagcagt tcaccaagga tcagatgtct cacctgttca atgtggcaca cacactgcgt
     6301 atgatggtgc agaaggagcg gagcctcgac atcctgaagg ggaaggtcat ggcctccatg
     6361 ttctatgaag tgagcacacg gaccagcagc tcctttgcag cagccatggc ccggctggga
     6421 ggtgctgtgc tcagcttctc ggaagccaca tcgtccgtcc agaagggcga atccctggct
     6481 gactccgtgc agaccatgag ctgctatgcc gacgtcgtcg tgctccggca cccccagcct
     6541 ggagcagtgg agctggccgc caagcactgc cggaggccag tgatcaatgc tggggatggg
     6601 gtcggagagc accccaccca ggccctgctg gacatcttca ccatccgtga ggagctggga
     6661 actgtcaatg gcatgacgat cacgatggtg ggtgacctga agcacggacg cacagtacat
     6721 tccctggcct gcctgctcac ccagtatcgt gtcagcctgc gctacgtggc acctcccagc
     6781 ctgcgcatgc cacccactgt gcgggccttc gtggcctccc gcggcaccaa gcaggaggaa
     6841 ttcgagagca ttgaggaggc gctgcctgac actgatgtgc tctacatgac tcgaatccag
     6901 aaggaacgat ttggctctac ccaggagtac gaagcttgct ttggtcagtt catcctcact
     6961 ccccacatca tgacccgggc caagaagaag atggtggtga tgcacccgat gccccgtgtc
     7021 aacgagataa gcgtggaagt ggactcggat ccccgcgcag cctacttccg ccaggctgag
     7081 aacggcatgt acatccgcat ggctctgtta gccaccgtgc tgggccgttt cgactacaaa
     7141 gacgatgacg acaagtagga attcctgcag gcctcgaggg ccggcgcgcc gcggccgcta
     7201 cgtaaattcc gccccccccc ccctctccct cccccccccc taacgttact ggccgaagcc
     7261 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     7321 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     7381 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     7441 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     7501 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     7561 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     7621 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     7681 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     7741 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc
     7801 acaaccatgg ttcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca
     7861 atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac
     7921 ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc
     7981 gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc
     8041 ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat
     8101 ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg
     8161 attcgtgaat tgctgccctc tggttatgtg tgggagggct aagtcgacga taaaataaaa
     8221 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     8281 gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa
     8341 gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt
     8401 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc
     8461 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc
     8521 ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc
     8581 caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt
     8641 ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg
     8701 cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc
     8761 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact
     8821 acccgtcagc gggggtcttt cacatgcagc atgtatcaaa attaatttgg ttttttttct
     8881 taagtattta cattaaatgg ccatagttgc attaatgaat cggccaacgc gcggggagag
     8941 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     9001 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     9061 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     9121 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     9181 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     9241 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     9301 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     9361 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     9421 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     9481 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     9541 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     9601 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9661 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9721 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9781 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9841 tgcggccggc cgcaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9901 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9961 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    10021 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    10081 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    10141 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    10201 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    10261 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    10321 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    10381 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    10441 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10501 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10561 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10621 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10681 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10741 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10801 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10861 cacatttccc cgaaaagtgc cacctgcata atgaaagacc ccacctgtag gtttggcaag
    10921 ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag aatagaaaag
    10981 ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
    11041 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca
    11101 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc
    11161 cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc
    11221 aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc
    11281 tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggcgc
    11341 gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca
    11401 gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta
    11461 cccgtcagcg ggggtctttc atttgggggc tcgtccggga tcgggagacc cctgcccagg
    11521 gaccaccgac ccaccaccgg gaggtaagct ggccagcaac ttatctgtgt ctgtccgatt
    11581 gtctagtgtc tatgactgat tttatgcgcc tgcgtcggta ctagttagct aactagctct
    11641 gtatctggcg gacccgtggt ggaactgacg agttcggaac acccggccgc aaccctggga
    11701 gacgtcccag ggacttcggg ggccgttttt gtggcccgac ctgagtccaa aaatcccgat
    11761 cgttttggac tctttggtgc acccccctaa taggagggat atgtggttct ggtaggagac
    11821 gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg ggaccgaagc
    11881 cgcgccgcgc gtcttgtctg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt
    11941 ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt
    12001 cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt
    12061 tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc
    12121 acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat
    12181 ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct
    12241 ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg
    12301 t
//
