LOCUS       sequence-340983-        2591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. 
            Participates in DNA replication; helps to specify the origin of 
            genomic replication..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2591)
  TITLE     Open Enzyme Collection
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pOpen-RNAse H
FEATURES             Location/Qualifiers
     source          1..2591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(614..632)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      628..675
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(680..696)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(680..696)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(763..1351)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(843..862)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1542..2333,2334..2402))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2165..2184
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2403..2507)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2523..2539
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      2540..2571
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 aaaggggccg tcaatatcag ggtctctaat gctgaaacag gtagaaatct tcactgacgg
       61 ttcctgcctg ggcaacccgg gtccgggcgg ttatggtgca attctgcgct accgtggacg
      121 cgaaaaaacc ttctctgccg gctacacccg taccaccaac aaccgcatgg aattgatggc
      181 agctatcgtg gcgctggaag ccctgaagga acactgtgaa gttatcctgt ccaccgatag
      241 ccagtacgta cgtcaaggta tcacccagtg gattcacaac tggaaaaaac gcggctggaa
      301 aacggctgac aaaaaaccgg tcaagaacgt agacctgtgg cagcgtctgg acgcggccct
      361 tgggcagcac cagatcaaat gggaatgggt taagggtcac gcaggccacc cagaaaacga
      421 acgttgtgat gaactggcac gtgccgcagc aatgaacccc actttggagg acaccggtta
      481 tcaggtggaa gtgtgaagag ctttgagacc aggacaccag accaatgaag gaaagcgggt
      541 caacgcgccg gtagggataa cagggtaatc tacacagaca tccagcaaaa cgctgaggtg
      601 tcaatcgtcg gagccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
      661 ttgaggggtt ttttgcatgg tcatagctgt ttcctgagag cttggcaggt gatgacacac
      721 attaacaaat ttcgtgagga gtctccagaa gaatgccatt aatttccata ggctccgccc
      781 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      841 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      901 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      961 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1021 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1081 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1141 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1201 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1261 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1321 gcagattaca cgcagaaaaa aaggatctca agaaggccta ctattagcaa caacgatcct
     1381 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1441 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     1501 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     1561 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1621 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1681 cgagaaccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1741 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1801 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1861 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1921 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1981 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     2041 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     2101 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     2161 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     2221 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     2281 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     2341 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     2401 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     2461 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac ctgcaccagt
     2521 cagtaaaacg acggccagta gtcaaaagcc tccgaccgga ggcttttgac ttggttcagg
     2581 tggagtggga g
//
