LOCUS       sequence-340988-        4778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNA-dependent RNA polymerase derived from T3 bacteriophage that 
            catalyzes the transcription of DNA into RNA using the four 
            ribonucleoside triphosphates as substrates..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4778)
  TITLE     Open Enzyme Collection
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pOpen-T3gene1
FEATURES             Location/Qualifiers
     source          1..4778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2801..2819)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2815..2862
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(2867..2883)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2867..2883)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(2950..3538)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3030..3049)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3729..4520,4521..4589))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4352..4371
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4590..4694)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4710..4726
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      4727..4758
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 aaaggggccg tcaatatcag ggtctctaat gaacatcatt gagaatattg aaaaaaatga
       61 tttctcggaa atcgaactgg ctgctattcc gtttaacacc ctggcagatc actacggctc
      121 ggcgctggct aaagaacagc tggccctgga acacgaaagc tacgaactgg gtgaacgtcg
      181 tttcctgaaa atgcttgaac gtcaggcgaa agctggcgaa attgcggaca acgcggctgc
      241 caaaccgctg ctggctaccc tgctgcctaa gctgacaacc cgtattgtag aatggttaga
      301 agaatatgct tctaaaaaag gtcgcaaacc gtctgcgtac gcgccactgc aactgctgaa
      361 accggaggca agcgctttca tcaccctgaa agttatcctg gcgagcttga ccagcactaa
      421 catgactacc atccaggcgg cggcgggtat gctgggcaaa gccatcgaag atgaagcgcg
      481 tttcggccgc atccgcgacc tggaagcaaa acatttcaag aaacacgttg aagaacaact
      541 gaacaaacgc cacggtcagg tgtacaaaaa agccttcatg caggtcgttg aggcggacat
      601 gatcggtcgt ggtctgctgg gtggtgaagc atggagctct tgggataaag agactactat
      661 gcacgtcggc attcgcctaa tcgaaatgct gatcgaaagc actggtctgg ttgaactgca
      721 acgtcacaac gccggcaacg caggcagcga ccacgaagct ctccaactgg cccaggaata
      781 cgttgatgtt ttggcaaaac gcgcaggtgc tcttgctggc atctccccga tgtttcagcc
      841 gtgcgttgtc ccgccgaaac cgtgggttgc cattaccggc ggcggttatt gggctaacgg
      901 tcgtcgcccg ctggcgttag ttcgtactca ctctaagaaa ggcctgatgc gttacgaaga
      961 tgtgtacatg ccggaggttt ataaggcagt gaacctggcg cagaacaccg catggaaaat
     1021 caacaaaaaa gtactggccg ttgtcaatga aatcgtgaac tggaaaaact gcccggtagc
     1081 cgacatccca tccctggaac gtcaagaact gccgcctaaa ccggacgaca tcgacactaa
     1141 cgaagcggcg ctgaaagaat ggaaaaaagc ggcggcgggt atctatcgcc tggacaaggc
     1201 gcgtgtaagc cgtcgtatca gcctggagtt catgctggag caggctaaca aatttgcgag
     1261 caagaaagca atctggttcc cgtataacat ggattggcgc gggcgtgtgt acgctgtacc
     1321 aatgtttaac ccgcagggca acgatatgac taaaggtctg ctgaccttag caaaaggtaa
     1381 gccgattggc gaagaaggct tctactggct gaagatccac ggtgcaaatt gtgcgggtgt
     1441 tgacaaagtt ccgtttccgg aacgtatcgc attcatcgaa aaacatgtag acgacatcct
     1501 ggcgtgtgca aaagacccaa ttaacaacac ctggtgggcg gaacaggact ctccgttctg
     1561 ctttctggct ttctgtttcg aatacgcggg cgtaacccac catggtctgt cttacaactg
     1621 ttctctgccg ctggcttttg acggtagctg ctctggtatt cagcacttca gcgctatgct
     1681 gcgcgacgaa gtaggcggcc gtgctgttaa cctgcttccg tctgaaaccg ttcaagatat
     1741 ttacggtatt gtggcgcaaa aagtgaacga aatccttaaa caagacgcga tcaatgggac
     1801 accgaacgag atgatcactg ttaccgataa agacaccggc gaaattagcg aaaaactgaa
     1861 actgggtacc tcaactctgg cacagcagtg gctggcatac ggcgtgactc gctctgtgac
     1921 aaaacgctcc gttatgaccc tcgcatacgg ctctaaagag ttcggctttc gtcagcaagt
     1981 tctggacgac accatccagc cggcaatcga ttccggtaaa ggcctgatgt ttacccagcc
     2041 gaaccaggcc gctggctata tggcgaaact gatatgggat gcagtttctg tgaccgtagt
     2101 agcggctgta gaggcgatga attggctgaa atctgcggcc aaactgctgg cagctgaggt
     2161 gaaagacaaa aaaaccaaag aaatcttgcg ccatcgctgc gcagtgcact ggactacccc
     2221 ggacggcttc ccagtgtggc aggaataccg taaaccgctc cagaaacgtc tggacatgat
     2281 cttcctcggt cagttccgtt tgcagccgac cattaacacc ctgaaagatt ctggcatcga
     2341 cgcgcataaa caggaatcag gtatcgcccc gaacttcgtg cattctcagg atggtagcca
     2401 cttgcgcatg accgtagttt acgcacacga aaaatacggc atcgaaagct ttgcgctgat
     2461 ccacgactct ttcgggacca tcccggcgga cgctggcaaa ctcttcaaag cagttcgtga
     2521 aaccatggtt atcacctacg aaaacaacga cgttctggca gacttctact ctcagttcgc
     2581 tgatcaactg cacgaaaccc agctggacaa aatgccgccg ctgccgaaaa aaggtaacct
     2641 gaacctgcaa gatatcctga aaagcgactt cgcgttcgcc tgaagagctt tgagaccagt
     2701 cccaagttca gacgtacccc accaatagac aacgcggcta gggataacag ggtaatcgct
     2761 cctttagatc caggcaccgc tgaggtgtca atcgtcggag ccgctgagca ataactagca
     2821 taaccccttg gggcctctaa acgggtcttg aggggttttt tgcatggtca tagctgtttc
     2881 ctgagagctt ggcaggtgat gacacacatt aacaaatttc gtgaggagtc tccagaagaa
     2941 tgccattaat ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     3001 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     3061 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     3121 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     3181 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     3241 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     3301 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3361 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     3421 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3481 tagcggtggt ttttttgttt gcaagcagca gattacacgc agaaaaaaag gatctcaaga
     3541 aggcctacta ttagcaacaa cgatcctttg atcttttcta cggggtctga cgctcagtgg
     3601 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     3661 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     3721 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     3781 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     3841 tctggcccca gtgctgcaat gataccgcga gaaccacgct caccggctcc agatttatca
     3901 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3961 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     4021 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     4081 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     4141 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     4201 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     4261 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     4321 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     4381 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     4441 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     4501 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     4561 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     4621 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     4681 ataggggttc cgcgcacctg caccagtcag taaaacgacg gccagtagtc aaaagcctcc
     4741 gaccggaggc ttttgacttg gttcaggtgg agtgggag
//
