LOCUS       sequence-340993-       12557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CDKL5 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12557)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 12557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-CDKL5_IDG-K
FEATURES             Location/Qualifiers
     source          1..12557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     475..495
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             716..949
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1021..1142
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1058..1077)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1112..1131
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1182..1317
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1337..1356)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1338..1356)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1366..1383)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1366..1382)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1375..1397)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1524..1979
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1611..1630)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1821..1842
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2005..2109
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2110..2178,2179..2970)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3141..3729
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3630..3649
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3883..3900
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4017..4038
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4053..4058,4059..4076,4077..4083)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4091..4107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4096..4118
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4115..4131
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4115..4131
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4150..4170
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4152..4170
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4198..4424
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4425..4605
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4652..4777
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5270..5503
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5688..5732
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5999..6115
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6149..6169
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6169..7380
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7217..7236
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(7384..7404)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      7405..7414
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7417..8187
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             8194..8235
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    8236..8260
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(11360..11384)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    11414..12002
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11467..11487)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11885..11896)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        12016..12515
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(12051..12074)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     12448..12467
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(12536..12557,1..578)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(12536..12555)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
       61 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
      121 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
      181 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
      241 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
      301 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
      361 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
      421 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
      481 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
      541 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc
      601 gcccgaccga aaggagcgca cgaccccatg catctcgagg gcccggtacc tttaagacca
      661 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
      721 gggctagctc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
      781 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      841 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      901 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
      961 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     1021 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     1081 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     1141 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     1201 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     1261 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     1321 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     1381 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     1441 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     1501 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     1561 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     1621 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     1681 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     1741 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     1801 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     1861 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     1921 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     1981 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     2041 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     2101 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     2161 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     2221 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     2281 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     2341 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     2401 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     2461 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     2521 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     2581 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     2641 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     2701 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     2761 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     2821 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     2881 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     2941 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     3001 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     3061 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     3121 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3181 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3241 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     3301 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3361 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3421 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3481 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3541 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3601 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3661 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3721 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3781 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3841 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3901 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     3961 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     4021 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     4081 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     4141 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt
     4201 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
     4261 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
     4321 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
     4381 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
     4441 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     4501 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     4561 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     4621 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     4681 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     4741 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     4801 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     4861 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     4921 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     4981 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     5041 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     5101 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     5161 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     5221 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     5281 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     5341 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     5401 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     5461 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     5521 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     5581 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     5641 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     5701 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     5761 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     5821 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     5881 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     5941 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     6001 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     6061 caacagacat acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcgt
     6121 ccagtgtggt ggaattctgc agatatcaca actttgtata gaaaagttgg cctccgcgcc
     6181 gggttttggc gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg
     6241 aagggcgcag cgagcgtcct gatccttccg cccggacgct caggacagcg gcccgctgct
     6301 cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga cgggacttgg
     6361 gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa aagtagtccc
     6421 ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga ttatataagg
     6481 acgcgccggg tgtggcacag ctagttccgt cgcagccggg atttgggtcg cggttcttgt
     6541 ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct gctgggctgg ccggggcttt
     6601 cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga gagaccgcca agggctgtag
     6661 tctgggtccg cgagcaaggt tgccctgaac tgggggttgg ggggagcgca gcaaaatggc
     6721 ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac
     6781 aaggtggggg gcatggtggg cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg
     6841 aaagctctta ttcgggtgag atgggctggg gcaccatctg gggaccctga cgtgaagttt
     6901 gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc
     6961 gttgggcagt gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc
     7021 gttctgttgg cttataatgc agggtggggc cacctgccgg taggtgtgcg gtaggctttt
     7081 ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg acaggcgccg
     7141 gacctctggt gaggggaggg ataagtgagg cgtcagtttc tttggtcggt tttatgtacc
     7201 tatcttctta agtagctgaa gctccggttt tgaactatgc gctcggggtt ggcgagtgtg
     7261 ttttgtgaag ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt
     7321 cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac
     7381 ggccaacttt tgtatacaaa gtgggccacc atgggaatcc cgctgctgaa cgctaaacag
     7441 attctgggac agctggacgg cgggagcgtg gcagtcctgc ctgtggtcga ctccaccaat
     7501 cagtacctgc tggatcgaat cggcgagctg aagagtgggg atgcttgcat tgcagaatat
     7561 cagcaggcag ggagaggaag cagagggagg aaatggttct ctccttttgg agctaacctg
     7621 tacctgagta tgttttggcg cctgaagcgg ggaccagcag caatcggcct gggcccggtc
     7681 atcggaattg tcatggcaga agcgctgcga aagctgggag cagacaaggt gcgagtcaaa
     7741 tggcccaatg acctgtatct gcaggataga aagctggcag gcatcctggt ggagctggcc
     7801 ggaataacag gcgatgctgc acagatcgtc attggcgccg ggattaacgt ggctatgagg
     7861 cgcgtggagg aaagcgtggt caatcagggc tggatcacac tgcaggaagc agggattaac
     7921 ctggacagga atactctggc cgctatgctg atccgagagc tgcgggcagc cctggaactg
     7981 ttcgagcagg aaggcctggc tccatatctg tcacggtggg agaagctgga taacttcatc
     8041 aatagacccg tgaagctgat cattggggac aaagagattt tcgggattag ccgggggatt
     8101 gataaacagg gagccctgct gctggaacag gacggagtta tcaaaccctg gatgggcgga
     8161 gaaatcagtc tgcggtctgc cgaaaaggct agcggcaagc ccatccccaa ccccctgctg
     8221 ggcctggaca gcaccacaag tttgtacaaa aaagcaggct ccaccatgaa gattcctaac
     8281 attggtgatg tgatgaataa atttgagatc cttggggttg taggtgaagg agcctatgga
     8341 gttgtactta aatgcagaca caaggaaaca catgaaattg tggcgatcaa gaaattcaag
     8401 gacagtgaag aaaatgaaga agtcaaagaa acgactttac gagagcttaa aatgcttcgg
     8461 actctcaagc aggaaaacat tgtggagttg aaggaagcat ttcgtcggag gggaaagttg
     8521 tacttggtgt ttgagtatgt tgaaaaaaat atgctcgaat tgctggaaga aatgccaaat
     8581 ggagttccac ctgagaaagt aaaaagctac atctatcagc taatcaaggc tattcactgg
     8641 tgccataaga atgatattgt ccatcgagat ataaaaccag aaaatctctt aatcagccac
     8701 aatgatgtcc taaaactgtg tgactttggt tttgctcgta atctgtcaga aggcaataat
     8761 gctaattaca cagagtacgt tgccaccaga tggtatcggt ccccagaact cttacttggc
     8821 gctccctatg gaaagtccgt ggacatgtgg tcggtgggct gtattcttgg ggagcttagc
     8881 gatggacagc ctttatttcc tggagaaagt gaaattgacc aactttttac tattcagaag
     8941 gtgctaggac cacttccatc tgagcagatg aagcttttct acagtaatcc tcgcttccat
     9001 gggctccggt ttccagctgt taaccatcct cagtccttgg aaagaagata ccttggaatt
     9061 ttgaatagtg ttctacttga cctaatgaag aatttactga agttggaccc agctgacaga
     9121 tacttgacag aacagtgttt gaatcaccct acatttcaaa cccagagact tctggatcgt
     9181 tctccttcaa ggtcagcaaa aagaaaacct taccatgtgg aaagcagcac attgtctaat
     9241 agaaaccaag ccggcaaaag tactgctttg cagtctcacc acagatctaa cagcaaggac
     9301 atccagaacc tgagtgtagg cctgccccgg gctgacgaag gtctccctgc caatgaaagc
     9361 ttcctaaatg gaaaccttgc tggagctagt cttagtccac tgcacaccaa aacctaccaa
     9421 gcaagcagcc agcctgggtc taccagcaaa gatctcacca acaacaacat accacacctt
     9481 cttagcccaa aagaagccaa gtcaaaaaca gagtttgatt ttaatattga cccaaagcct
     9541 tcagaaggcc cagggacaaa gtacctcaag tcaaacagca gatctcagca gaaccgccac
     9601 tcattcatgg aaagctctca aagcaaagct gggacactgc agcccaatga aaagcagagt
     9661 cggcatagct atattgacac aattccccag tcctctagga gtccctccta caggaccaag
     9721 gccaaaagcc atggggcact gagtgactcc aagtctgtga gcaacctttc tgaagccagg
     9781 gcccaaattg cggagcccag taccagtagg tacttcccat ctagctgctt agacttgaat
     9841 tctcccacca gcccaacccc caccagacac agtgacacga gaactttgct cagcccttct
     9901 ggaagaaata accgaaatga gggaacgctg gactcacgtc gaaccacaac cagacattct
     9961 aagacgatgg gggaattgaa gctgccggag cacatggaca gtagccattc ccattcactg
    10021 tctgcacctc acgaatcttt ttcttatgga ctgggctaca ccagcccctt ttcttcccag
    10081 caacgtcctc ataggcattc tatgtatgtg acccgtgaca aagtgagagc caagggcttg
    10141 gatggaagct tgagcatagg gcaagggatg gcagctagag ccaacagcct gcaactcttg
    10201 tcaccccagc ctggagaaca gctccctcca gagatgactg tggcaagatc ttcggtcaaa
    10261 gagacctcca gagaaggcac ctcttccttc catacacgcc agaagtctga gggtggagtg
    10321 tatcatgacc cacactctga tgatggcaca gcccccaaag aaaatagaca cctatacaat
    10381 gatcctgtgc caaggagagt tggtagcttt tacagagtgc catctccacg tccagacaat
    10441 tctttccatg aaaataatgt gtcaactaga gtttcttctc taccatcaga gagcagttct
    10501 ggaaccaacc actcaaaaag acaaccagca ttcgatccat ggaaaagtcc tgaaaatatt
    10561 agtcattcag agcaactcaa ggaaaaagag aagcaaggat ttttcaggtc aatgaaaaag
    10621 aaaaagaaga aatctcaaac agtacccaat tccgacagcc ctgatcttct gacgttgcag
    10681 aaatccattc attctgctag cactccaagc agcagaccaa aggagtggcg ccccgagaag
    10741 atctcagatc tgcagaccca aagccagcca ttaaaatcac tgcgcaagtt gttacatctc
    10801 tcttcggcct caaatcaccc ggcttcctca gatccccgct tccagccctt aacagctcaa
    10861 caaaccaaaa attccttctc agaaattcgg attcaccccc tgagccaggc ctctggcggg
    10921 agcagcaaca tccggcagga acccgcaccg aagggcaggc cagccctcca gctgccagac
    10981 ggtggatgtg atggcagaag acagagacac cattctggac cccaagatag acgcttcatg
    11041 ttaaggacga cagaacaaca aggagaatac ttctgctgtg gtgacccaaa gaagcctcac
    11101 actccgtgcg tcccaaaccg agcccttcat cgtccaatct ccagtcctgc tccctatcca
    11161 gtactccagg tccgaggcac ttccatgtgc ccgacactcc aggtccgagg cactgatgct
    11221 ttcagctgcc caacccagca atccgggttc tctttcttcg tgagacacgt tatgagggaa
    11281 gccctgattc acagggccca ggtaaaccaa gctgcgctcc tgacatacca tgagaatgcg
    11341 gcactgacgg gcaagtagga cccagctttc ttgtacaaag tggtgatatc cagcacagtg
    11401 gcggccgctc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
    11461 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
    11521 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
    11581 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
    11641 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
    11701 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
    11761 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
    11821 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
    11881 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
    11941 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
    12001 gcctggaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
    12061 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
    12121 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
    12181 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
    12241 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
    12301 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
    12361 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
    12421 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
    12481 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgac
    12541 cgagtacaag cccacgg
//
