LOCUS       sequence-341000-       14397 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses Rosa multiflora MYB108 in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14397)
  AUTHORS   Dong J, Cao L, Zhang X, Zhang W, Yang T, Zhang J, Che D
  TITLE     An R2R3-MYB Transcription Factor RmMYB108 Responds to Chilling 
            Stress of Rosa multiflora and Conferred Cold Tolerance of 
            Arabidopsis.
  JOURNAL   Front Plant Sci. 2021 Jul 27;12:696919. doi: 
            10.3389/fpls.2021.696919. eCollection 2021.
  PUBMED    34386027
REFERENCE   2  (bases 1 to 14397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2021-07-27"; volume: "12:696919. doi"; pages: " 
            10.3389/fpls.2021.696919. eCollection 2021"
COMMENT     Sequence Label: pCAMBIA1301-RmMYB108
FEATURES             Location/Qualifiers
     source          1..14397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             638..1663
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    1704..1878
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    1956..1980
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2405..3199
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3286..3874
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3775..3794
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4028..4045
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4060..4200
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4126..4145)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4286..4308
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4544..4738
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4804..5877)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6306..6935)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8236..8260
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(8282..8534)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8569..8586)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(8593..10410,10601..10615))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          10411..10600
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     promoter        complement(10632..10977)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     11339..11361
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11353..11370
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11354..11370
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11755..11801
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(13264..13283)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(13991..14007)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13991..14007)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14004..14026)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14015..14031
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14039..14045,14046..14063,14064..14069))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14084..14105
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14296..14397,1..571)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct ctgccgacag
       61 tggtcccaaa gatggacacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
      121 tcttcaaagc aagtggattg atgtgaacat ggtggagcac gacactctcg tctactccaa
      181 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
      241 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac
      301 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt
      361 tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
      421 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
      481 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
      541 aagttcattt catttggaga ggacacgctg aaatcaccag tctctctcta caaatctatc
      601 tctctcgagc tttcgcagat ccggggggca atgagatatg aaaaagcctg aactcaccgc
      661 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
      721 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
      781 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
      841 atcggccgcg ctcccgattc cggaagtgct tgacattggg gagtttagcg agagcctgac
      901 ctattgcatc tcccgccgtt cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
      961 gcccgctgtt ctacaaccgg tcgcggaggc tatggatgcg atcgctgcgg ccgatcttag
     1021 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     1081 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     1141 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     1201 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     1261 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     1321 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     1381 cttcgagcgg aggcatccgg agcttgcagg atcgccacga ctccgggcgt atatgctccg
     1441 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     1501 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     1561 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
     1621 tggaaaccga cgccccagca ctcgtccgag ggcaaagaaa tagagtagat gccgaccggg
     1681 atctgtcgat cgacaagctc gagtttctcc ataataatgt gtgagtagtt cccagataag
     1741 ggaattaggg ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat
     1801 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc
     1861 agtactaaaa tccagatccc ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc
     1921 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
     1981 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
     2041 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
     2101 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
     2161 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
     2221 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
     2281 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
     2341 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
     2401 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
     2461 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
     2521 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
     2581 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
     2641 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
     2701 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
     2761 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
     2821 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
     2881 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
     2941 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     3001 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     3061 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     3121 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     3181 actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc
     3241 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3301 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3361 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3421 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3481 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3541 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3601 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3661 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3721 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3781 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3841 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3901 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     3961 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     4021 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     4081 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     4141 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     4201 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     4261 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     4321 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
     4381 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
     4441 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
     4501 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
     4561 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
     4621 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
     4681 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
     4741 aaagagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
     4801 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
     4861 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
     4921 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
     4981 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
     5041 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
     5101 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
     5161 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
     5221 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
     5281 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg
     5341 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
     5401 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
     5461 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
     5521 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
     5581 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa
     5641 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg
     5701 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg
     5761 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag
     5821 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgctcacgcc
     5881 gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcaggcaac ccagccgctt
     5941 acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt
     6001 tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca
     6061 tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt
     6121 gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt
     6181 gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg
     6241 tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca
     6301 ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg
     6361 tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc
     6421 acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca
     6481 acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc
     6541 cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat
     6601 atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac
     6661 acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg
     6721 gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct
     6781 tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca
     6841 tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac
     6901 ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat
     6961 ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac
     7021 atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg
     7081 ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg
     7141 cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga
     7201 aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt
     7261 ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg
     7321 cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg
     7381 atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc
     7441 gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg
     7501 ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc
     7561 ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca
     7621 cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag
     7681 taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg
     7741 gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt
     7801 ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc
     7861 ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat
     7921 ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac
     7981 aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga
     8041 catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg
     8101 gaggggttgg atcaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac
     8161 aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat tctaataaac
     8221 gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaattcc
     8281 cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt
     8341 ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc
     8401 ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat
     8461 tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa
     8521 atgtttgaac gatcggggaa attcgagctg gtcacctgta attcacacgt ggtggtggtg
     8581 gtggtggcta gcttgtttgc ctccctgctg cggtttttca ccgaagttca tgccagtcca
     8641 gcgtttttgc agcagaaaag ccgccgactt cggtttgcgg tcgcgagtga agatcccttt
     8701 cttgttaccg ccaacgcgca atatgccttg cgaggtcgca aaatcggcga aattccatac
     8761 ctgttcaccg acgacggcgc tgacgcgatc aaagacgcgg tgatacatat ccagccatgc
     8821 acactgatac tcttcactcc acatgtcggt gtacattgag tgcagcccgg ctaacgtatc
     8881 cacgccgtat tcggtgatga taatcggctg atgcagtttc tcctgccagg ccagaagttc
     8941 tttttccagt accttctctg ccgtttccaa atcgccgctt tggacatacc atccgtaata
     9001 acggttcagg cacagcacat caaagagatc gctgatggta tcggtgtgag cgtcgcagaa
     9061 cattacattg acgcaggtga tcggacgcgt cgggtcgagt ttacgcgttg cttccgccag
     9121 tggcgcgaaa tattcccgtg caccttgcgg acgggtatcc ggttcgttgg caatactcca
     9181 catcaccacg cttgggtggt ttttgtcacg cgctatcagc tctttaatcg cctgtaagtg
     9241 cgcttgctga gtttccccgt tgactgcctc ttcgctgtac agttctttcg gcttgttgcc
     9301 cgcttcgaaa ccaatgccta aagagaggtt aaagccgaca gcagcagttt catcaatcac
     9361 cacgatgcca tgttcatctg cccagtcgag catctcttca gcgtaagggt aatgcgaggt
     9421 acggtaggag ttggccccaa tccagtccat taatgcgtgg tcgtgcacca tcagcacgtt
     9481 atcgaatcct ttgccacgca agtccgcatc ttcatgacga ccaaagccag taaagtagaa
     9541 cggtttgtgg ttaatcagga actgttcgcc cttcactgcc actgaccgga tgccgacgcg
     9601 aagcgggtag atatcacact ctgtctggct tttggctgtg acgcacagtt catagagata
     9661 accttcaccc ggttgccaga ggtgcggatt caccacttgc aaagtcccgc tagtgccttg
     9721 tccagttgca accacctgtt gatccgcatc acgcagttca acgctgacat caccattggc
     9781 caccacctgc cagtcaacag acgcgtggtt acagtcttgc gcgacatgcg tcaccacggt
     9841 gatatcgtcc acccaggtgt tcggcgtggt gtagagcatt acgctgcgat ggattccggc
     9901 atagttaaag aaatcatgga agtaagactg ctttttcttg ccgttttcgt cggtaatcac
     9961 cattcccggc gggatagtct gccagttcag ttcgttgttc acacaaacgg tgatacgtac
    10021 acttttcccg gcaataacat acggcgtgac atcggcttca aatggcgtat agccgccctg
    10081 atgctccatc acttcctgat tattgaccca cactttgccg taatgagtga ccgcatcgaa
    10141 acgcagcacg atacgctggc ctgcccaacc tttcggtata aagacttcgc gctgatacca
    10201 gacgttgccc gcataattac gaatatctgc atcggcgaac tgatcgttaa aactgcctgg
    10261 cacagcaatt gcccggcttt cttgtaacgc gctttcccac caacgctgat caattccaca
    10321 gttttcgcga tccagactga atgcccacag gccgtcgagt tttttgattt cacgggttgg
    10381 ggtttctaca ggacggacga gtcgtcggtt ctgtaactat catcatcatc atagacacac
    10441 gaaataaagt aatcagatta tcagttaaag ctatgtaata tttacaccat aaccaatcaa
    10501 ttaaaaaata gatcagttta aagaaagatc aaagctcaaa aaaataaaaa gagaaaaggg
    10561 tcctaaccaa gaaaatgaag gagaaaaact agaaatttac cctcagatct accatggtca
    10621 agagtccccc gtgttctctc caaatgaaat gaacttcctt atatagagga agggtcttgc
    10681 gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact
    10741 tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt
    10801 ccatctttgg gaccactgtc ggcagaggca tcttcaacga tggcctttcc tttatcgcaa
    10861 tgatggcatt tgtaggagcc accttccttt tccactatct tcacaataaa gtgacagata
    10921 gctgggcaat ggaatccgag gaggtttccg gatattaccc tttgttgaaa agtctcaatt
    10981 gccctttggt cttctgagac tgtatctttg atatttttgg agtagacaag tgtgtcgtgc
    11041 tccaccatgt tgacgaagat tttcttcttg tcattgagtc gtaagagact ctgtatgaac
    11101 tgttcgccag tctttacggc gagttctgtt aggtcctcta tttgaatctt tgactccatg
    11161 aagctaaact gaaggcggga aacgacaatc tgatccaagc tcaagctgct ctagcattcg
    11221 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
    11281 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
    11341 cagtcacgac gttgtaaaac gacggccagt gccaagcttc gtcaacatgg tggagcacga
    11401 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
    11461 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
    11521 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
    11581 taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc
    11641 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
    11701 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
    11761 cccttcctct atataaggaa gttcatttca tttggagagg agctcccgcg ggtcgacggt
    11821 accatggatg tcaaaccagg aagggtttcg gacatcatta gaaacagttc tgaggaagac
    11881 gaagatgcta tgatgatgga cctgagaaga gggccatgga cggttgagga agacgttgtt
    11941 ctcatgaatt acattgctaa ccacggcgaa ggtcgctgga actcccttgc tcgctgtgca
    12001 ggtctgaaaa gaactggaaa gagctgcaga ttaaggtggc tcaactatct ccgacccgac
    12061 gttcgacgtg gcaacatcac tctcgaagaa cagcttctca ttcttgagct tcattctcgc
    12121 tggggcaacc gatggtccaa aattgcacag cacttgcccg gaaggactga caatgaaatc
    12181 aaaaactact ggaggactcg cgtgcaaaaa cacgcaaagc agctcaagtg tgacgtcaac
    12241 agcaagcagt tcaaggacac catgcgctac ctctggatgc caagattggt cgagagaatt
    12301 caagccgccg cgtccgccgc caccgccccc accggagccc cttcttctat cacaaccgcc
    12361 gcggccaaca cttatcattt caacaataac aataacagct tccaagcccc cgcgggagca
    12421 ggacacgtcg ctttacaaca gccaacgccc attggcaata acaatgactt ggccggaagt
    12481 tacaccactc cggaaaactc cagcactgcg gcgtcatcgg actcgtttgg gactcaggtc
    12541 tcgcctgtgt cagagctaac cgactattac agtaatatgt cggttaacaa taataaacct
    12601 agtagtcagg ctctggatta cttccaagcc accaatcctc atcaccaagt cggttatcat
    12661 gattccatga ctagtccatc tggttactca ttcaatcaag gaggactaaa cagccataac
    12721 tttcaagctg cttcggcgcc ggagcaaaac aacaacggcc agtggagcat ggacggtggg
    12781 gacttttcgg acaatctgtg gaacgttgag gaatccgaca tgtggttctt acagcagcaa
    12841 ctcggcaata ttggatcctg tacagcggcc gcttcaggga gtggttccgg aagcggctct
    12901 ggatcgggct cagggagtgg ttccggaagc ggctctggat cggctgccgc agcaatttgt
    12961 atcgtgaagg gcgaggaact cttcaccggc gtggtgccga tcctcgtgga gctcgacggc
    13021 gacgtgaacg gccacaagtt ctccgtgtcc ggcgagggcg agggcgacgc cacctacggc
    13081 aagctcaccc tcaagttcat ctgcaccacc ggcaagctcc cggtgccgtg gccgaccctc
    13141 gtgaccacct tctcctacgg cgtgcagtgc ttctcccgct acccggacca catgaagcgc
    13201 cacgacttct tcaagtccgc catgccggag ggctacgtgc aggagcgcac catcttcttc
    13261 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctcgtg
    13321 aaccgcatcg agctcaaggg catcgacttc aaggaggacg gcaacatcct cggccacaag
    13381 ctcgagtaca actacaactc ccacaacgtg tacatcatgg ccgacaagca gaagaacggc
    13441 atcaaggcca acttcaagac ccgccacaac atcgaggacg gcggcgtgca gctcgccgac
    13501 cactaccagc agaacacccc gatcggcgac ggcccggtgc tcctcccgga caaccactac
    13561 ctctccaccc agagcgccct ctccaaggac ccgaacgaga agcgcgacca catggtgctc
    13621 ctcgagttcg tgaccgccgc cggcatcacc cacggcatgg acgagctcta caactgatgc
    13681 aggtgagagc acctcacctg caaaactgca ggctagcagt actcgttcaa acatttggca
    13741 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
    13801 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
    13861 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
    13921 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcggggaat
    13981 tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
    14041 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
    14101 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
    14161 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattggctag agcagcttgc
    14221 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
    14281 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
    14341 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcac
//
